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All Journal Kultivasi Life Science and Biotechnology
Ziyaroh, Putry Lazimatu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Propagation of Dendrobium crumenatum Sw. through In Vitro Seed Culture Using Fertilizer-Enriched and Organic Complex Media Su'udi, Mukhamad; Ziyaroh, Putry Lazimatu
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53562

Abstract

Dendrobium crumenatum Sw. is one of the epiphytic orchids with high ecological and economic value. This study aims to examine the effect of media containing organic compounds enriched with fertilizers for in vitro propagation of D. crumenatum seeds. This culture medium contains organic materials such as potatoes, bananas, coconut water, charcoal, sucrose, and foliar fertilizer. This medium was designed to provide macro, micro, and growth hormone nutrients that support explant growth. The research procedure included sterilization of orchid seeds, explant sowing, and growth observation for about two months. The results showed that orchid seeds experienced optimal growth in media with the addition of coconut water and other organic compounds. The formulation of organic-based media with the addition of coconut water and other supporting compounds is effective in supporting the in vitro growth of orchids, providing opportunities for efficient propagation of orchid species. Explant growth reached 1-2 cm at the 68th day after sowing, with a fresh green colour as an indicator of optimal physiological conditions. The even distribution of shoots indicates that the media provides sufficient space and nutrients without competition between explants. In sum, simple tissue culture media produced from a mixture of organic materials proved reliable function and suited for growing D. crumenatum orchid seeds to produce plantlets, and into further extent it would support conservation efforts. Key words: Conservation, organic materials, pigeon orchid, simple media, tissue culture
Morphology and bioactive compounds of gurmar (Gymnema sylvestre) with potential as antidiabetics Yuliany, Neny Novita; Farhan, Abdillah Maulana; Ziyaroh, Putry Lazimatu; Dwinianti, Edia Fitri; Nihayah, Husnatun; Su'udi, Mukhamad
Kultivasi Vol 25, No 1 (2026)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v25i1.64921

Abstract

The medicinal plant Gymnema sylvestre is widely known for its ability to lower blood sugar levels. This study reviewed the physical characteristics and bioactive compounds of G. sylvestre sourced from different regions, emphasizing its potential as an antidiabetic agent. Morphological analysis includes leaf structure, trichome presence, and other anatomical features, which can influence the plant's chemical profile. Phytochemical analysis, performed using techniques like GC-MS, identifies key bioactive compounds such as saponins, flavonoids, and gymnemic acids, which contribute to its hypoglycemic effect. The findings indicate that regional variations can affect the concentration and presence of these compounds, suggesting that environmental factors and genetic diversity play significant roles. This comprehensive characterization supports the use of G. sylvestre as a natural antidiabetic treatment, which supports its potential as a source of antidiabetic-related bioactive compounds. Further research is recommended to explore the pharmacological mechanisms and optimize the extraction methods for maximum efficacy.
Co-Authors Dwinianti, Edia Fitri Farhan, Abdillah Maulana Nihayah, Husnatun Su'udi, Mukhamad Yuliany, Neny Novita