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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOEDUKASI Jurnal Biologi Tropis Jurnal Sains dan Edukasi Sains
Dwinianti, Edia Fitri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Physics Other

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Genetic Variation Analysis of EMS-Induced Chili Pepper (Capsicum frutescens L.) Mutants Using SSR Markers Dwinianti, Edia Fitri; Mastuti, Retno; Arumingtyas, Estri Laras
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.472 KB)

Abstract

Mutation induction with chemical mutagen ethyl methane sulfonate (EMS) capable of producing genetic variation in plants. The purpose of this research was to evaluate genetic variation of EMS-induced chili pepper mutants using SSR molecular marker. Various concentration of EMS (0.01%; 0.02%; 0.04%) were applied to chili pepper seeds of Genotypes 2, 7, and 11. The genomic DNA was isolated from mutants and control plants then used for PCR analysis using three SSR primers namely CA26, CA52 and CA96. The three SSR primers were able to detect genetic variation in the three genotypes of EMS-induced chili pepper mutants and control plants. A total of 18 alleles have been identified in all three SSR loci with PIC values ranging from 0.792 to 0.893. The three genotypes of chili pepper plants had different sensitivity to EMS mutation induction. Genotype 11 plants showed higher sensitivity to EMS treatment than genotypes 2 and 7 based on the amount of genomic changes. Dendrogram constructed based on genetic similarity values distributed the mutants and control plants into three main groups with similarity coefficients ranging from 0.34 to 0.90. Similar genotype either control plant or mutants, especially Genotypes 2 and 7 were group into similar group. However, for the Genotype 11 plants, large genome changes cause mutant plants G11K1, G11K2, G11K3 had a low genetic similarity to their control plants, so the mutants were separated in a different group from the control plant. Those indicate that EMS mutation induction capable of increasing genetic variation in chili pepper plants based on SSR molecular marker.
Molecular identification of Cyclosorus parasiticus (L.) Farw. From Gumitir, Jember Indonesia Rizqoni, Moh Ikmal Alfi; Setyati, Dwi; Su'udi, Mukhamad; Ulum, Fuad Bahrul; Dwinianti, Edia Fitri
BIOEDUKASI Vol 22 No. 2 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i2.45484

Abstract

Cyclosorus parasiticus (L.) Farw. from Mount Gumitir dominates the understory ferns vegetation along the roadside of a pine-mixed coffee plantation. Previous studies reported the metabolite profile with the flavonoid and alkaloid content. Current observations based on morphological structure of the specimens collection indicated a high variations, therefor molecular identification was required to support the phylogenetic data of the species. The aim of the study was to identify the Cyclosorus parasiticus sample originate from Gumitir using DNA barcode. We used three primers, i.e. ribulose 1,5-biphosphate carboxylase (rbcL), maturase K (matK), and Internal transcribed spacer 2 (ITS2) for molecular identification. The result showed that Cyclosorus parasiticus from Gumitir has a highest similarity with Christella latipinna (NC070300) from China with a value of Percent Identities value of 99.11% based on the ITS2 marker. The rbcL data in this study might contribute to supporting the molecular genus concept from recent Thelypteridaceae phylogeny.
Studi Etnofarmakologi Potensi Terminalia arjuna (Roxb. Ex DC) Wight & Arn dan Perkembangannya dalam Bidang Kesehatan Su'udi, Mukhamad; Khoirunnisa, Gita Ayu; Ramadany, Zakiyah; Ardiyanti, Kurnia Dwi; Dwinianti, Edia Fitri
Jurnal Sains dan Edukasi Sains Vol. 8 No. 1 (2025): Jurnal Sains dan Edukasi Sains
Publisher : Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/juses.v8i1p79-87

Abstract

Tanaman herbal telah dimanfaatkan manusia dalam bidang pengobatan untuk menyembuhkan berbagai macam penyakit dan ilmu pengobatan tradisional telah berlangsung sejak zaman kuno hingga saat ini. Terminalia arjuna merupakan salah satu tanaman yang banyak digunakan dalam obat-obatan herbal tradisional Ayurveda dari India. Pengolahan T. arjuna dalam pengobatan Ayurveda yaitu dikonsumsi dengan cara diseduh atau dikonsumsi bersama mentega (ghee) dan susu (ksheerapaka). Kulit batang T. Arjuna adalah bagian yang paling banyak dimanfaatkan oleh masyarakat India sebagai obat. Berbagai senyawa metabolit yang telah diisolasi dari akar, batang, daun, buah, dan biji dan telah diuji dalam skala laboratorium, praklinis dengan hewan uji maupun uji klinis pada manusia untuk mengetahui potensi terapeutiknya. Senyawa metabolit yang ditemukan meliputi flavonoid, terpenoid, polifenol, glikosida dan steroid beserta derivatnya. Kandungan senyawa metabolit dan potensi aktivitas terapeutik melalui berbagai penelitian dikumpulkan menggunakan ulasan ilmiah menggunakan kajian dari berbagai sumber literasi. Hasil berbagai penelitian menunjukkan T. arjuna berpotensi sebagai agen terapeutik yang berperan penting dengan kemampuan menghambat maupun menyembuhkan berbagai gangguan kesehatan. Aktivitas farmakologis T. Arjuna menunjukkan potensi terapeutik dalam aktivitas antikanker, antimikrob, antioksidan, serta memberikan efek kardioprotektif, hepatoprotektif dan gastroprotektif. Studi etnofarmakologi potensi terapeutik T. arjuna diharapkan mampu memberikan dasar pengetahuan pemanfaatan T. arjuna sebagai bahan dasar obat herbal di masa yang akan datang.
DNA Barcode Analysis of Dendrobium sp. Based On rbcL Sequence Dwinianti, Edia Fitri; Durotun Ainiyah; Yusfi Afidah; Mukhamad Su'udi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10177

Abstract

Morphological characterization of Dendrobium orchids needs to be supported by the utilization of DNA barcoding as a faster and more accurate approach. This study aims to identify the rbcL marker as a potential barcode for Dendrobium sp. collected from Jenggawah, Jember. The isolation of Dendrobium sp. genomic DNA was performed using the CTAB method, and the isolated DNA was amplified using PCR. The PCR products were used for sequencing analysis, and the sequence data were analyzed using several bioinformatics programs namely BLAST, ClustalX 2.1, and MEGA11. The results showed that the Dendrobium sp. sequence had a high level of homology with Calanthe alpina (MN192480.1), with a genetic similarity of 99.82%. The sequence analysis results of rbcL in Dendrobium sp. showed low genetic variation, which made rbcL less effective for use as a molecular marker at the species level. The rbcL barcode showed universal results for Dendrobium sp., so it needs to be supported by molecular identification using other markers such as matK and ITS for a more effective identification method in distinguishing between species.
Co-Authors Ardiyanti, Kurnia Dwi Durotun Ainiyah Dwi Setyati Estri Laras Arumingtyas Fuad Bahrul Ulum Khoirunnisa, Gita Ayu Mukhamad Su'udi Ramadany, Zakiyah Retno Mastuti Rizqoni, Moh Ikmal Alfi Su'udi, Mukhamad Yusfi Afidah