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Iwanuddin , Iwanuddin Iwanuddin
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Identification of Water Bacteria from Nickel Post Mining in East Halmahera Margaretta, Margaretta Christita; Iwanuddin , Iwanuddin Iwanuddin; Yermias, Yermias Kafiar; Supratman , Supratman Tabba
Jurnal Wasian Vol. 5 No. 1 (2018): June
Publisher : Forestry Department, University of Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62142/x32gnn20

Abstract

Nickel mining impact in heavy metal pollution on both soil and water. A method that is widely applied to reduce heavy metal contamination is bioremediation. Selection of bacteria that have potential reduction of heavy metal contamination in soil is very important in bioremediation process. The first step to select the potential bacteria is identification of samples. The purpose of this study is to identify existings bacteria in pond after nickel mining area PT. Antam, East Halmahera. Methods used in this research are isolation and identification of bacteria by conventional methods includes morphological, physiologica,l and biochemical test. Identification using Bergey’s Manual Determinative. The results showed there are 6 genera types of bacteria there are Bacillus, Esherichia, Enterococcus, Pseudomonas, Staphylococcus, dan Klebsiella, with 18 species in water of nickel post mining. Morphologically it is dominated by genus Bacillus as 50 % of the species. The identification of bacteria proved an existence of indigenous bacteria which is resistant to heavy metal stress.
Effect of Fruit Maturity and Extraction Treatment on Germination Percentage of Langusei (Ficus minahassae (Teysm.et.Vr.) Miq)) Irawan, Arif Irawan; Iwanuddin , Iwanuddin Iwanuddin; Jafred , Jafred Elsjoni Halawane; Fuad, Fuad Muhammad
Jurnal Wasian Vol. 7 No. 2 (2020): December
Publisher : Forestry Department, University of Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62142/qm2s0687

Abstract

Langusei (Ficus minahassae (Teysm.et.Vr.) Miq) is one of the endemic flora of Sulawesi which existence is increasingly threatened. Attempts to maintain the existence of Ficus minahassae species by understanding the cultivation techniques of the langusei species, especially regarding the appropriate germination techniques, need to be addressed. This study aims to determine the level of fruit maturity and the appropriate extraction treatment for the germination of langusei seeds. The experimental design used in this study was a complete randomization design which is arranged in a factorial pattern. The first factor is the classification of the level of seed maturity based on fruit color categories: 1) Orange-brown fruit, 2) Orange-reddish fruit, 3) Red fruit, and 4) Red-black fruit, while the second factor is the fruit extraction treatment which consists of: 1) Extraction with dry wind treatment for 24 hours, 2) Extraction with drying treatment for 12 hours, and 3) Extraction with water treatment for 24 hours. The results showed that the fruit maturity level and the appropriate extraction treatment to produce optimal langusei seed germination were in the orange-brown and orange-reddish fruit (mature fruits prior to ripening) which was soaked in water for 24 hours.