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All Journal Jurnal Ilmiah Hijau Cendekia Journal of Biodiversity and Biotechnology
SKD, Tyas
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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EFEKTIVITAS PENAMBAHAN MIKORIZA DAN EKSTRAK TOMAT PADA MEDIA AKLIMATISASI TERHADAP PERTUMBUHAN PLANLET PISANG (Musa.sp) Sari, Siti Mardhika; Aziez, Achmad Fatchul; Suprapti, Endang; SKD, Tyas; Daryanti, Daryanti; Wiyono, Wiyono
Jurnal Ilmiah Hijau Cendekia Vol 10 No 2 (2025): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v10i2.7978

Abstract

ABSTRAK Aklimatisasi merupakan tahap kritis dalam perbanyakan tanaman secara in vitro karena planlet harus beradaptasi dari kondisi steril ke lingkungan eksternal yang lebih bervariasi, sehingga rentan terhadap stres lingkungan. Penelitian ini bertujuan untuk mengevaluasi efektivitas penambahan mikoriza dan ekstrak tomat pada media aklimatisasi terhadap pertumbuhan planlet pisang (Musa sp.) hasil kultur jaringan. Penelitian dilaksanakan menggunakan Rancangan Acak Kelompok (RAK) dua faktor, yaitu dosis mikoriza (0 g, 5 g, 10 g/plant) dan konsentrasi ekstrak tomat (0%, 5%, 10%) dengan sembilan kombinasi perlakuan. Parameter pengamatan meliputi persentase bibit hidup, tinggi tanaman, diameter pangkal batang, dan jumlah daun aktif. Hasil penelitian menunjukkan bahwa kombinasi mikoriza dan ekstrak tomat berpengaruh nyata terhadap seluruh parameter pertumbuhan. Perlakuan tertinggi (M2E2) menghasilkan persentase hidup 99,2%, tinggi tanaman 27,2 cm, diameter pangkal batang 0,95 cm, dan jumlah daun aktif 7 helai. Peningkatan ini menunjukkan bahwa mikoriza berperan dalam memperluas serapan hara dan memperkuat sistem perakaran, sedangkan ekstrak tomat menyediakan hormon pertumbuhan dan antioksidan yang mendukung proses adaptasi. Temuan ini menegaskan bahwa kombinasi mikoriza 10 g dan ekstrak tomat 10% merupakan perlakuan terbaik untuk meningkatkan keberhasilan aklimatisasi planlet pisang secara efektif, efisien, dan ramah lingkungan, serta berpotensi diterapkan pada produksi bibit hortikultura lainnya. Kata Kunci : Mikoriza, ekstrak tomat, aklimatisasi, kultur jaringan, planlet pisang.
Effectiveness of Long Bean Plant Pest Control by Utilizing Cyanic Acid Content from Various Parts of Rubber Cassava Plants (Manihot Glaziovii) Sari, Siti Mardhika; Suprapti, Endang; SKD, Tyas; Aziez, Achmad Fatchul; Cahyani, Nonik Fitri
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.104966

Abstract

Pest attacks are one of the main obstacles in the cultivation of long beans (Vigna unguiculata ssp. sesquipedalis) in Indonesia. This study aims to evaluate the effectiveness of cyanide acid content from various parts of the rubber cassava plant (Manihot glaziovii) in controlling Aphis craccivora pests and its impact on the growth and yield of long beans. The study was conducted in Sragen and the Laboratory of the Faculty of Agriculture, Tunas Pembangunan University, Surakarta during September–December 2024 with a completely randomized block design (RAKL) with two factors, namely plant parts (leaves, tuber skin, and tubers) and extract doses (0, 10, 20, and 30 ml/plant). The results showed that tuber skin fermentation (B2) was the most effective in reducing the percentage of pest attacks (30.80%) and attack intensity (40.44%). The treatment dose of 30 ml/plant (K4) gave the best results in suppressing pest attacks and increasing growth and yield parameters, such as plant length (141.64 cm), fresh stalk weight (1905.77 g), and fruit weight (497.22 g). The bioactive content in cassava tuber skin has the potential as a pest control agent as well as a plant growth stimulant. This study supports the use of environmentally friendly and sustainable local biopesticides to increase the productivity of long beans.
Co-Authors Achmad Fatchul Aziez Cahyani, Nonik Fitri DARYANTI DARYANTI Endang Suprapti Siti Mardhika Sari Wiyono wiyono