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The use of alternative coloring variations to mitotic observations of shallots using the squash method Tirtasari, Ni Luh; Saputri, Luthfi Hanum; Dewi, Nur Kusuma; Siswanti, Siswanti
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 1 (2024): April 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i1.3053

Abstract

Practicum cannot be carried out due to limited facilities and infrastructure such as dyes for observation of mitotic cell chromosomes in onions. Alternative dyes that can be obtained from nature can be explored. The purpose of this study was to obtain preparation of the mitotic division process in various onion root cells using alternative dyes using dyes from dragon fruit peel and turi flower. In obtaining the right preparation using the squash method. Data were obtained using observation sheets that observed each variation of the material. The instrument used in this study is a valid observation sheet. The results showed that the coloring of the preparation using dragon fruit skin and turi flowers was successful and clear coloring results were obtained. Coloring in practicum activities using turi flowers is better than dragon fruit coloring. Natural dyes from dragon fruit peel and turi flower can be used as alternative dyes in the squash method to observe onion root mitosis, replacing synthetic dyes. 
Exploratory of Ecological Quality from Remote-Sensing Ecological Index and Drought Hazard in Pekalongan Regency, Indonesia Fariz, Trida Ridho; Naufal, Muhammad Ahganiya; Heriyanti, Andhina Putri; Eralita, Norma; Saputri, Luthfi Hanum
Journal of Geoscience, Engineering, Environment, and Technology Vol. 10 No. 3 (2025): JGEET Vol 10 No 03 : September (2025)
Publisher : UIR PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/jgeet.2025.10.3.22396

Abstract

Climate change has intensified environmental hazards, including floods, landslides, and droughts, with Pekalongan Regency, Indonesia, emerging as a vulnerable region facing these multifaceted challenges. While flood-related studies dominate existing study, drought impacts remain understudied, despite their growing prevalence. Current climate hazard assessments in Pekalongan's adaptation plans rely heavily on historical data, limiting their predictive accuracy. This study addresses these gaps by developing a Remote Sensing Ecological Index (RSEI) model to evaluate ecological quality and its association with drought hazards, aligning with climate-resilient development objectives. The study employs Landsat imagery to construct RSEI using four key indicators: NDVI (greenness), WET (wetness), NDBSI (dryness), and LST (heat). Drought hazard data were derived from 2023 disaster records provided by Pekalongan's Regional Disaster Management Agency (BPBD). Statistical analysis using chi-square tests examined the relationship between RSEI components and drought hazard classes.Results demonstrate that RSEI's first principal component (PC1) effectively captures spatial ecological patterns, with southern regions (notably Petungkriyono's tropical rainforest) exhibiting "good" to "excellent" conditions, while northern urbanized areas score lower ("fair" to "poor"). PC1 shows a statistically significant association with drought hazard, unlike PC2 or PC3, suggesting its utility as a drought vulnerability indicator. However, the chi-square approach only identifies categorical relationships without quantifying effect strength or direction, highlighting methodological limitations. This study contributes to climate adaptation science by validating RSEI's applicability for drought assessment in tropical coastal regions. Future study should incorporate ordinal regression or spatial modeling to enhance predictive capability. The findings support evidence-based policymaking for targeted mitigation in Pekalongan Regency and similar vulnerable regions, emphasizing the integration of ecological monitoring into climate adaptation frameworks.