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Wijayanti, Faulia Triana
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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In Vitro Anti-Biofilm Activity of Nutmeg (Myristica fragrans) Leaf Infusion Extract Wijayanti, Faulia Triana; Nugroho, Yusuf Eko; Pangesti, Ira
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2097

Abstract

Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium commonly associated with severe infections, particularly in immunocompromised individuals. One of its major virulence factors is biofilm formation, which contributes to increased antimicrobial resistance. This study aimed to evaluate the inhibitory activity of nutmeg (Myristica fragrans) leaf infusion extract against P. aeruginosa biofilm formation in vitro. The experiment was conducted using a 96-well microtiter plate assay, and biofilm intensity was quantified at 595 nm following crystal violet staining. The results demonstrated that the nutmeg leaf infusion extract significantly inhibited biofilm formation, with varying levels of effectiveness depending on incubation time. These findings indicate the potential of nutmeg leaf infusion extract as an antibiofilm agent against P. aeruginosa.
Co-Authors Ira Pangesti Nugroho, Yusuf Eko