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All Journal ACTA VETERINARIA INDONESIANA Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Puspitasari, Riris
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Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Perkembangan Sel Punca Mesenkimal Melalui Kultur Primer Eksplan Wharton’s Jelly Tali Pusat Sapi Puspitasari, Riris; Boediono, Arief; Prasetyaningtyas, Wahono; Subangkit, Mawar; Lindiawati, Riris
Acta VETERINARIA Indonesiana Vol. 12 No. 3 (2024): November 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.3.231-239

Abstract

Saat ini penggunaan sel punca mesenkimal Wharton’s jelly (SPMWJ) sapi dapat menjadi alternatif bagi terapi pet animals. Manfaat SPM antara lain meregulasi jalur persinyalan sel, rendahnya penolakan sistim imun pada pemberian alogenik, tidak adanya resiko keganasan, stabil penyimpanannya, dan mudah transportasinya. Kultur tali pusat sapi belum dilaporkan perkembangan eksplan WJ terutama proses pertumbuhan eksplan WJ mulai dari attach hingga sel konfluen. Tujuan penelitian adalah menentukan perkembangan eksplan WJ tali pusat sapi. Faktor yang dilihat diantaranya attachment eksplan, munculnya outgrowth, morfologi sel, dan konfluensi sel. Eksplan mampu attach sebanyak 42% pada hari ke-8 kultur dan 88% (hari ke-12). Outgrowth sel teramati pada 45% eksplan (hari ke-8) dan 100% (hari ke-12). Morfologi sel yang terlihat adalah fibroblastik. Konfluensi sel didapat pada hari ke-12, hari ke-16, dan hari ke-20 yaitu 3%, 83%, dan 100%.
Detection of Cytochrome B Pork in Packaged Meatball from Traditional Markets around Jakarta Kurniah, Allen Nurul; Puspitasari, Riris; Perdana, Analekta Tiara
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.931 KB)

Abstract

Meatballs are one of the favorite foods for Indonesian people. Meatball products are generally known to be based on beef. But there are cases that changed the basic ingredients of meatballs, beef into pork. This research was conducted to detect the presence of pork in packaged meatball products sold at Traditional Markets. Identification is carried out with the principles of molecular biology in the form of the PCR process. Meatball samples were previously subjected to DNA extraction. PCR was performed to determine pork genes using cytochrome b primers with an amplicon length of 130 bp. The cytochrome b gene can be found in every animal cell, but it has a specific characteristic that is only present in certain animal cells. Based on PCR results it is known that most of the samples did not contain pork marked by the absence of a DNA band that appeared and parallel to the 130 bp band on the ladder. However, there are two samples that appear DNA bands and are thought to contain pork. These results are followed by a sequencing process to clarify the results of the PCR process.
Detection of Pig Cytochrome B in Beef Sausage Found in Traditional Markets around Jakarta Dewi, Astria Prastika; Puspitasari, Riris; Perdana, Analekta Tiara
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.967 KB)

Abstract

Processed fast food products are currently in great demand by the people of Indonesia, especially with basic ingredients such as beef, chicken or other meats. Sausage is a fast food processed food that is at risk of mixing other food ingredients that can not be known by consumers. The halal of food products is considered very important by Muslim community and is taken into consideration in consuming food products. The food category in Indonesia still often mixes or falsifies halal food with prohibited ingredients, one of which is processed products such as sausages. Contamination of processed food products based on sausages was found to contain pig contamination circulating in the one of market in Indonesia by testing as many as 5 out of 6 sausage samples were detected positively contaminated with pig which resembled a Polymerase Chain Reaction (PCR) product sequencing results of 95% with Sus scrofa species. The purpose of this study is to detect pig contamination in processed food products such as sausages sold in traditional markets and modern markets and the benefits of this research to detect cytochrome b pig genes in sausages and provide information to the public about the halal status of sausages on the market. The research method uses the PCR Technique which is able to be used as a tool to detect contamination of other meats with using a pig specific primer. Sausage samples was obtained from around Jakarta. DNA of pigs and bovine was extracted as positive and negative controls, as well as testing all of sausage samples. The results showed that from all of sausage samples amplified by PCR there were no pig cytochrome b genes. This finding informed that the sausages collected from market around Jakarta were negative to pig components.
Co-Authors Arief Boediono Dewi, Astria Prastika Kurniah, Allen Nurul Lindiawati, Riris Perdana, Analekta Tiara Prasetyaningtyas, Wahono Subangkit, Mawar