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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Majalah Kedokteran Bandung Indonesian Journal of Biotechnology ARSHI Veterinary Letters Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan

Subangkit, Mawar

Sekolah Kedokteran Hewan Dan Biomedis, Institut Pertanian Bogor

Author-ID : 3654414

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay Wahyu Widowati; Diana Krisanti Jasaputra; Teresa Liliana Wargasetia; The Fransiska Eltania; Alya Mardhotillah Azizah; Mawar Subangkit; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ermi Girsang; Ahmad Faried
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.186

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.

Spatial learning and memory of young and aging rats following injection with human Wharton’s jelly‐mesenchymal stem cells Berry Juliandi; Wildan Mubarok; Dian Anggraini; Arief Boediono; Mawar Subangkit; Indra Bachtiar; Harry Murti; Kelvin Yaprianto; Boenjamin Setiawan
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64734

Human Wharton’s jelly‐mesenchymal stem cells (hWJ‐MSC) are an emerging potential source of stem cells derived from the umbilical cord. Previous studies have shown their potential as treatment for traumatic brain injury and Parkinson’s disease. However, no study has yet investigated the effect of hWJ‐MSC injections in countering spatial learning and memory impairment in aging rats. The effect of hWJ‐MSC injection on young rats is also unknown. The objective of this research was to analyze the effect of an hWJ‐MSC injection on spatial learning, memory, density of putative neural progenitor cells (pNPC), and neuronal apoptosis in the dentate gyrus (DG) of young and aging rats. Injection of hWJ‐MSC did not change spatial learning and memory in young rats until two months post‐injection. This might be due to retained pNPC density and neuronal apoptosis in the DG of young rats after injection of hWJ‐MSC. In contrast, injection of hWJ‐MSC promoted both spatial learning and memory in aging rats, a finding that might be attributable to the increased pNPC density and attenuated neuronal apoptosis in DG of aging rats during the two months post‐injection. Our study suggests that a single injection of hWJ‐MSC might be sufficient to promote improvement in long‐term learning and memory in aging rats.

Stabilitas Fisikokimia dan Sifat Antipenuaan Kulit Formula Krim Berbahan Aktif Alami (Physicochemical stabilities and Skin Antiaging Properties of Bioaktive-based Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Nopen Meisaroh; Ietje Wientarsih; D R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 1 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The purpose of this study was to analyze the physicochemical stabilities and in vivo antiaging properties of the cream formulas of F1, F2, and F3 which contained the active compound of 0.1% 0.18, and 0.36%, respectively. The active compound was the combination of ethyl acetate fraction of Toona sinensis, Centella asiatica, and Acacia mangium leaf (1:2:1). For physicochemical stability tests (color, odor, acidity, and antioxidant activity), the creams were stored for 21 days in the refrigerator, room temperature, and extreme temperature (±40 ⁰C). For in vivo antiaging test, the mice skin were smeared with cream and UV irradiated for 15 minutes per day. After two and four weeks, the mice skins were biopsied. The results showed that the physicochemical properties of creams stored in the refrigerator were stable. The storage of creams at room and extreme temperature decreased the physicochemical properties. The application of cream formulas on mice skin after two to four weeks were able to increase the thickness of epidermis, but has not been able to increase the collagen skin of mice.Keywords: Acacia mangium, Centella asiatica, ethyl acetate fraction of leaf, physicochemical and antiaging properties of cream, Toona sinensis

Aktivitas Antioksidan Ekstrak Daun Surian, Mangium, dan Pegagan serta Kombinasinya dalam Formula Krim (Antioxidant Activities of Mangium, Surian, and Pegagan Leaf Extracts and Its Combination in Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Itje Wintarsih; Dewi R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 14, No 2 (2016): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The simplicia properties of toona (TS), mangium (AM), and centella (CA) leaves and antioxidant activities of their ethanolic leave extract and their combinations in cream formulas were examined in the present reserach. The ethanolic extracts were partitioned with n-hexane and ethyl acetate. The antioxidant activity of the fractions and their combinations were tested against DPPH. The best combination was formulated in F1, F2, and F3 cream formulas with 0.1, 0.18, and 0.36% of extract, respectively. The formulas antioxidant activity was then tested. The results showed that the water content, ash content, and acidic insoluble ash content of TS, AM, and CA simplicias were ±10%, 3.8-9.7%, and 0.9-1.2% respectively. The yields of n-hexane (FN), ethyl acetate (FEA), and residues (FR) fractions were 6-9, 2, and 5-6% respectively. The antioxidant activity of FEA, FR, and FN was very strong, strong, and weak to inactive, respectively. The best combination was the mixture of 2/3CA, 1/6AM, and 1/6TS (IC50 9 μg ml-1). The antioxidant activity of all cream formulas were higher than refered commercial cream. The IC50 values of formula 1, 2, and 3 were 1109, 596, and 202 μg ml-1, respectively, while the IC50 of refered commercial cream was 2372 μg ml-1.Keywords: antioxidant, centella, cream, extract, mangium, toona

Efikasi Pemberian Maserasi Kemangi sebagai Antifertilitas terhadap Profil Hematologi dan Biokimia Darah Tikus Betina Andriyanto; Rindy Fazni Nengsih; Leliana Widi; Hamdika Yendri; Mawar Subangkit; Elpita Tarigan; Yusa Irarang; Aulia Andi Mustika; Lina Noviyanti Sutardi; Wasmen Manalu
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.270-274

Penelitian ini dilakukan untuk mengeksplorasi khasiat fitofarmaka lokal Indonesia yaitu kemangi sebagai antifertilitas dalam meningkatkan kesehatan dan keamanan tikus betina. Sebanyak 15 ekor tikus betina galur Sprague-Dawley (umur 8 minggu, bobot badan 220-230 g) dibagi acak ke dalam 3 perlakuan dan 5 ulangan, yaitu: kontrol (K0); kemangi 1% (K1); dan kemangi 5% (K5) dalam bentuk maserasi yang ditambahkan ke dalam air minum. Pemberian jamu dilakukan selama 20 hari dimulai pada fase diestrus. Parameter profil darah yang diamati adalah gambaran darah merah (jumlah butir darah merah, hematokrit, dan hemoglobin) dan diferensial darah putih (limfosit, monosit, neutrofil, eosinofil, basofil). Parameter keamanan diamati dengan melihat fungsi organ hati (SGPT, SGOT) dan ginjal (ureum, kreatinin). Hasil menunjukkan bahwa profil darah dan pengamatan fungsi hati dan ginjal pada kelompok tikus betina yang diberi kemangi tidak terdapat perbedaan nyata (P>0.05) dengan kelompok kontrol. Pemberian kemangi tidak memengaruhi kondisi fisiologis tikus betina dan tidak toksik sehingga dapat digunakan untuk meningkatkan kesehatan tikus betina.

Efek Pemberian Maserasi Taoge (Vigna radiata) terhadap Profil Hematologi dan Biokimia Darah Tikus Betina andriyanto Andriyanto; Leliana Nugrahaning Widi; Rindy Fazni Nengsih; Hamdika Yendri Putra; Mawar Subangkit; Elpita Tarigan; Yusa Irarang; Aulia Andri Mustika; Lina Noviyanti Sutardi; Wasmen Manalu
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73351

Taoge memiliki berbagai manfaat farmakologis yang berpotensi besar untuk meningkatkan kesehatan. Penelitian ini dilakukan untuk mengeksplorasi keamanan dari konsumsi maserasi taoge melalui pengamatan terhadap profil hematologi dan biokimia darah. Sebanyak 15 ekor tikus betina galur Sprague Dawley berumur 8 minggu, memiliki bobot badan 220-230 g, dan belum pernah bunting dikelompokkan secara acak ke dalam 3 kelompok perlakuan (n=5 tikus), yaitu kelompok kontrol (diberi air mineral); kelompok maserasi taoge 1%; dan kelompok maserasi taoge 5%. Pemberian maserasi dilakukan melalui air minum dan dilaksanakan selama 20 hari. Hasil menunjukkan bahwa secara umum perlakuan maserasi taoge tidak memberikan pengaruh nyata (P>0.05) terhadap profil hematologi dan biokimia darah tikus percobaan. Terdapat peningkatan signifikan (P>0.05) pada kadar hemoglobin pada kelompok maserasi taoge 1% dibandingkan dengan kontrol yang lebih tinggi dari nilai normal. Hal yang serupa juga terjadi pada nilai neutrofil yang lebih tinggi dari nilai normal namun tidak berbeda nyata dengan kontrol (p>0.05). Selain keduanya, semua parameter menunjukkan nilai yang tidak berbeda nyata dibandingkan dengan kelompok kontrol dan berada pada rentang nilai normal.. Hal ini mengindikasikan bahwa pemberian maserasi tauge tidak memberikan efek negatif dan toksik pada dosis 1 dan 5% terhadap profil darah serta fungsi organ hati dan ginjal.

Distribusi Penyebaran Bovine Viral Diarrhea Virus (BVDV) pada Sapi Potong Impor asal Australia Di Sukabumi Berdasarkan Uji Serologis Aditya Primawidyawan; Retno Wulansari; Surachmi Setiyaningsih; Mawar Subangkit; Bambang Pontjo Priosoeryanto
Acta VETERINARIA Indonesiana Vol. 11 No. 1 (2023): Maret 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.1.51-62

Virus Bovine Viral Diarrhea (BVDV) secara luas diakui memiliki dampak ekonomi yang signifikan pada ternak yang terinfeksi. Kerugian penyakit ini meliputi, hewan Persistenly Infection (PI) yang kurang sehat, penyakit reproduksi, penurunan produksi, pertumbuhan yang buruk, dan berpengaruh immunosupressif terhadap hewan ternak. Penelitian ini bertujuan untuk melakukan suatu kajian pola distribusi penyebaran dengan karakterisasi gejala klinis serta mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Jumlah hewan yang diambil 100 ekor sapi yang didapatkan dengan metode penghitungan kajian lintas seksional dengan pertimbangan penyakit ini sudah ada di Indonesia. Persebaran penyakit pada setiap kandang kandang A sampai H dengan total 80 ekor sapi terlihat sampel positif merata sebanyak 21 sampel pada posisi nilai S-N sebesar 0,3-0,7 di pengambilan awal dan meningkat pada nilai S-N sebesar 0,9-1,5 sebanyak 53 sampel. Peningkatan penyebaran pada kandang diluar kandang karantina mencapai 2,5x lipat mengindikasikan penyebaran BVDV cukup tinggi. Pengelolaan limbah buruk akan berpeluang 2,483 kali lebih besar menimbulkan hasil ELISA antigen positif bila dibandingkan dengan peternakan yang memiliki pengelolaan limbah yang baik (OR=2,483; CI=1,066-5,783). Program biosekuriti buruk akan berpeluang 2,667 kali lebih besar menimbulkan hasil ELISA Ag BVD positif dibandingkan dengan peternakan yang memiliki program biosekuriti yang baik (OR=2,667; CI=1,145-6,210). Bangsa Brahman Cross berpeluang tiga kali lebih besar memiliki peluang hasil positif bila dibandingkan dengan sapi yang memiliki ras non-Brahman Cross (OR=3; CI=1,269-7,091). Sedangkan umur sapi > dua tahun akan berpeluang 3,241 dibandingkan dengan umur sapi yang < dua tahun (OR= 3,241; CI=1,411-8,912). Impak studi penelitian terhadap jurnal ini adalah memberikan informasi distribusi penyebaran penyakit BVD asal dari hewan sapi impor dari Australia yang berguna sebagai pertimbangan tindakan preventif dalam upaya pengendalian penyakit.

ACUTE TOXICITY TEST OF ETHANOLIC EXTRACT OF Acalypha hispida LEAVES IN FEMALE RATS: A PHYSIOLOGICAL AND HISTOLOGICAL STUDY Hamzah Alfarisi; Mawar Subangkit; Siti Sa’diah; Tutik Wresdiyati
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

This research aims to evaluate the safety of ethanolic extract of Acalypha hispida (A. hispida) leaves with acute toxicity test using 15 female rats strain Sprague-Dawley. A single dose of different doses of extract (2, 4, 8, and 16 g/kg body weight) was administrated orally, and theobservation was conducted for 14 days. The results revealed that the ethanolic extract of A. hispida leaves was relatively harmless (LD50 16 g/kg BW), did not affect body weight, and did not show clinical signs of toxicity during the observation periods. The parameters of blood serumbiochemistry of all extract-treated groups (alanine aminotransferase, aspartate aminotransferase, creatinine, and urea) did not change significantly compared to the control group. The histological observation of the liver showed a significant increase in eosinophilic cytoplasm and basophilic nuclei at all doses. However, the ethanolic extract of A. hispida leaves did not significantly affect glomerulus/Bowman’s capsule ratio, glomerular cell density, and the proportion of normal cell tubule. In conclusion, the ethanolic extract of A. hispida leaves was relatively harmless with LD5016 g/kg BW and seems to be safe in low doses (2 g/kg BW).

Hepatoprotective Potentials of Dates Extract (Phoenix dactylifera) in Acetaminophen-Induced Mice Andriyanto Andriyanto; Hamdika Yendri Putra; Sharon Aurelia; Aulia Andi Mustika; Wasmen Manalu; Mawar Subangkit; Elpita Tarigan; Yusa Irarang
Majalah Kedokteran Bandung Vol 55, No 3 (2023)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v55n3.3155

Dates (Phoenix dactylifera) are considered as a well-known fruit consumed by many people in various countries. This study aimed to examine potential effects of dates as a hepatoprotective agent in mice. This study was conducted at the Iratco Group’s eLRosa Laboratory Research Facility, Indonesia, starting from June 2022 to July 2022. Mice from ddY strain were randomly divided into 5 groups (n=5 per group) of positive and negative control groups, and treatment groups 1, 2, and 3. The negative control group as the normal baseline did not receive acetaminophen and date extract. In treatment groups, 30 µL/30 gr BW, 60 µL/30 gr BW, and 100 µL/30 gr BW extract was given per oral to Treatment Group 1, Group 2, and Group 3, respectively for 20 days. On day 21, all treatment groups were induced with 300mg/KgBW acetaminophen for 3 days via the intraperitoneal route. Blood tests were performed on day 24 to measure the serum transaminase level as the parameter of liver damage. The lowest level of transaminase serum was found in group 3 with the highest volume of dates extract, which was 100 µL, followed by group 2 (60 µL), and group 1 (30 µL). There was a significant difference between the positive control group and treatment groups with no significant difference was seen between negative and the treatment groups. This study concludes that dates extract has the potential of being a hepatoprotective agent.

Efek Pemberian Kombinasi Ekstrak Kemangi dan Tauge Terhadap Profil Hematologi dan Biokimia Darah Tikus Betina Andriyanto; Hamdika Yendri; Rindy Fazni Nengsih; Leliana Widi; Mawar Subangkit; Elpita Tarigan; Yusa Irarang; Lina Noviyanti Sutardi; Wasmen Manalu; Ridi Arif; Christina Clarice Leksono
Acta VETERINARIA Indonesiana Vol. 11 No. 2 (2023): Juli 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.2.160-166

Masalah fertilitas telah banyak diteliti dan banyak penanganan yang telah tersedia, namun pengobatan yang digunakan banyak berupa terapi hormon yang dapat menimbulkan efek samping buruk, sehingga menimbulkan pasar untuk alternatif pengobatan herbal. Penelitian ini bertujuan untuk menguji efek sediaan simplisia kemangi dan tauge terhadap profil heamtologi dan biokimia induk tikus. Penelitian digunakan menggunakan 15 ekor tikus galur Sprague-Dawley yang dibagi rata menjadi tiga kelompok, yakni satu kelompok kontrol normal, kelompok dosis 1% dan dosis 5%. perlakuan dengan dosis berbeda. Administrasi sediaan dilakukan selama 20 hari dengan mencampurkan 5 mL sediaan dalam 100 mL air minum. Pengamatan hematologi dan biokimia darah dilakukan pada hari ke-21. Data hasil pengujian dianalisis secara statistik menggunakan uji one way Analysis of Variance (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil penelitian tidak menunjukkan adanya perbedaan nyata (p>0.05) baik pada parameter hematologi maupun biokimia darah antara kelompok mencit yang diberi sediaan simplisia kemangi dan tauge dosis 1% dan 5% dengan kelompok kontrol. Hal ini menunjukkan bahwa sediaan memiliki potensi untuk menunjang kesehatan dan fertilitas tanpa risiko keamanan penggunaan karena tidak mengganggu fungsi hati maupun ginjal. Hasil penelitian diharapkan dapat memberi gambaran untuk penggunaan kombinasi kemangi dan tauge untuk menunjang fertilitas pada perempuan.

Co-Authors Aditya Primawidyawan Ahmad Faried Alya Mardhotillah Azizah Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Anita Esfandiari Arief Boediono Aulia Andi Mustika Aulia Andri Mustika Aurellia, Sharon Bambang Pontjo Priosoeryanto Berry Juliandi Boenjamin Setiawan Christina Clarice Leksono Cintya Nur Nabilla D R Agungpriyono Deded Sarip Nawawi Deni Noviana Dertina Marni Purba Dewi R Agungpriyono Dian Anggraini Diana Krisanti Jasaputra Dodi Irwan Suparno Drajat Martianto Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Elpita Tarigan Ermi Girsang Eva Harlina Fitri, Arni Diana Ginting, Chrismis Novalinda Gunanti Gunanti . Hamdika Yendri Hamdika Yendri Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamdika Yendri Putra Hamzah Alfarisi Harry Murti I Nyoman Ehrich Lister I Nyoman Jaya Wistara IETJE WIENTARSIH Indra Bachtiar Itje Wintarsih Jerica, Monica Silva Jurnila Sari Tanjung Kelvin Yaprianto L N Sutardi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Widi Leliana Widi Lina Noviyanti Sutardi Lindiawati, Riris Maulana, M Ridha Mayasari, Ni Luh Putu Ika Naufal Muharam Nurdin Nopen Meisaroh Nugraha, Arifin Budiman Prasetyaningtyas, Wahono Rahayu WoroWiranti Retno Wulansari Ridi Arif Rimbawan , Rindy Fazni Nengsih Rindy Fazni Nengsih Rindy Fazni Nengsih Risa Tiuria Rita Kartika Sari Shafiyyah Az Zahra Sharon Aurelia Sharon Aurelia Siti Sadiah Siti Sa’diah Sri Anna Marliyati Sri Rezeki Surachmi Setiyaningsih Teresa Liliana Wargasetia The Fransiska Eltania Tutik Wresdiyati Vetnizah Juniantito Vetty Ramadhaniah Wahyu Widowati Wasmen Manalu Wildan Mubarok Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang Yusa Irarang

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