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All Journal Jurnal Akademika Kimia
Ahmar, Dewi S.
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Chemistry Education

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Protein Content and Organoleptic Test Central Sulawesi Pumpkin Seed Tempeh (Cucurbita moschata) for Learning Resource Jannah, Raodatul; Santoso, Tri; Pulukadang, Sri H. V.; Ahmar, Dewi S.
Jurnal Akademika Kimia Vol. 14 No. 2 (2025)
Publisher : Universitas Tadulako

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Abstract

The yellow pumpkin (Cucurbita moschata) is a plant that readily adapts to diverse soil conditions; however, the utilization of its seeds remains restricted. Despite possessing a relatively high protein content crucial for growth and energy, it can serve as an alternative raw material for tempeh production. This study intends to generate yellow pumpkin seed tempeh in compliance with SNI 3144:2015 organoleptically, assess variations in the protein content of yellow pumpkin seed tempeh from three distinct regions in Central Sulawesi, and produce a Student Worksheet as an alternative resource for chemistry education through expert lecturer validation. This research employs a quantitative methodology utilizing the Kjeldahl analysis technique to ascertain protein concentrations across three phases: destruction, distillation, and titration. Furthermore, a sensory evaluation was conducted by 20 panelists to analyze the texture, color, flavor, and scent of yellow pumpkin seed tempeh. The resultant tempeh has favorable organoleptic properties, achieving an average preference score of 4, characterized by uniform white coloration, a distinctive tempeh aroma, and a dense texture. Protein content research revealed that tempeh from Palu City had the most significant protein concentration at 24.5 %, followed by Palolo District at 23.91 %, and North Lore District at 20.41 %, suggesting the impact of environmental factors on the nutritional composition of the manufactured tempeh. According to SNI 3144:2015, the visual characteristics of yellow pumpkin seed tempeh and its protein content conform to the established quality criteria. The findings of this study were presented as Student worksheets, which achieved a validity rate of 85 %, indicating its high suitability as a learning resource. This research enhances the application of yellow pumpkin seeds as a nutritious food source and fosters the creation of engaging and novel educational materials.
Soybean Tempeh: Effect of Yeast Concentration and Fermentation Duration on Organoleptic Test as a Learning Resource Mufida; Santoso, Tri; Pulukambang, Sri H. V.; Ahmar, Dewi S.
Jurnal Akademika Kimia Vol. 14 No. 2 (2025)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24775185.2025.v14.i2.pp70-76

Abstract

Tempeh is a traditional Indonesian fermented food known for its high nutritional value. This study sought to assess the impact of yeast concentration and fermentation length on moisture content, protein content, and organoleptic evaluation of yellow soybean tempeh sourced from Tojo Una-Una, Poso, and Palu. The research included a 3 × 3 factorial design incorporating yeast concentrations of 1, 2, and 3 grams, alongside fermentation durations of 30, 48, and 72 hours. The moisture content was assessed using the gravimetric method, the protein content was determined via the Kjeldahl method, and 20 panelists performed an organoleptic evaluation. The findings indicated that the moisture content of tempeh generally rose with prolonged fermentation time and elevated yeast concentration. The maximum moisture content, 63.22%, was observed in tempeh from Tojo Una-Una, which used 2 grams of yeast and had a fermentation duration of 72 hours. The minimum value, 50.18%, was also from Tojo Una-Una with 1 gram of yeast and 30 hours of fermentation. Protein levels peaked at 48 hours of fermentation with 3 grams of yeast, reaching a maximum of 26.88% in the Palu area. In contrast, the minimum was recorded at 18.55% in tempeh from Tojo Una-Una with 2 grams of yeast and 30 hours of fermentation. The organoleptic test results indicated that panelists favored Poso tempeh in terms of flavor, aroma, and texture. The concentration of yeast and the time of fermentation influence the moisture content, protein content, and organoleptic quality of tempeh. The methodologies and findings of this study serve as a resource for chemistry education in the format of LKPD.
Co-Authors Mufida Pulukadang, Sri H. V. Pulukambang, Sri H. V. Raodatul Jannah Tri Santoso