This study aims to analyze the effect of using digital-based learning media, the Kahoot application, and learning motivation on the learning interests of fifth-grade students at SD Katolik 03 Frater Don Bosco Manado. The study used a quantitative approach with a non-probability sampling method, a saturated sampling technique, involving 75 students as samples. The data were analyzed using descriptive statistics, correlation, reliability, normality, linearity, simple regression, and multiple regression with the help of SPSS 25. The results of the study showed that: (1) there was a significant direct effect of digital-based learning media, the Kahoot application, on students' learning interests with a significance value of p = 0.000 <0.05 and a Determinant Coefficient (R²) of 77.8%, which means that the Kahoot media explains 77.8% of the variation in students' learning interests; (2) learning motivation has a significant direct effect on learning interests with p = 0.000 <0.05 and R² = 79.6%, indicating that learning motivation explains 79.6% of the variation in learning interests; (3) Simultaneously, Kahoot learning media and learning motivation together have a significant effect on learning interest with p = 0.000 < 0.05 and R² = 80.1%, which means that the two variables explain 80.1% of the variation in students' learning interest in science subjects. In conclusion, the use of digital learning media based on the Kahoot application and learning motivation significantly increases the learning interest of class V students at SD Katolik 03 Frater Don Bosco Manado.