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All Journal Buletin Veteriner Udayana
Luh Gde Sri Surya Heryani
Laboratorium Anatomi dan Embriologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, 80234, Indonesia
Immunology & microbiology Public Health Veterinary

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HISTOLOGICAL STRUCTURE OF BALI DUCKS SKIN IN GROWER PHASE Dionysius Immanuel; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Ketut Berata; Luh Gde Sri Surya Heryani; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p07

Abstract

The skin is the largest and outermost organ that can be directly observed. The skin plays a crucial role for bali ducks as a protective barrier against the environment, maintaining body temperature, retaining body fluids, and neurosensory functions. This study aims to determine the structure of the bali duck skin (Anas sp.) during the grower phase. This study used ten bali ducks aged 3 months. The histological structure was observed using a binocular light microscope. The histological structure was analyzed descriptively and qualitatively. The structure of bali duck skin consists of three layers: epidermis, dermis, and hypodermis. The epidermis has three layers: stratum corneum, stratum intermedium, and stratum basale. The dermis has two layers: stratum compactum and stratum laxum. The tissues found in the bali duck skin include feather follicles, blood vessels, Herbst corpuscles, connective tissue, and musculus erector pili. Based on the research conducted, no differences in the histological structure of the skin were found across the three regions. Further research is needed on the skin structure of Bali ducks at different phases and sexes to obtain more comprehensive data, and specific histological studies are required to identify the skin histology of Bali ducks in greater detail.
PRESERVATION OF CANINE FRONT AND HIND LIMB SPECIMENS USING PLASTINATION TECHNIQUE: IMPREGNATION PHASE IN A PASSIVE VACUUM CHAMBER Luh Gde Sri Surya Heryani; I Nengah Wandia; I Ketut Suatha; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p08

Abstract

The plastination technique is a preservation method that involves the infusion of polymer materials into biological specimens to maintain their structural integrity, prevent decay, and ensure long-term durability. This technique is widely regarded as an effective approach for preserving organs and is particularly valuable for anatomical education. The objective of this study is to evaluate the qualitative characteristics—specifically texture, color, and odor—of plastinated canine front and hind limb specimens during the impregnation phase, conducted within a passive vacuum chamber. Additionally, this research aims to propose recommendations for refining plastination techniques to enhance the quality of preserved organ specimens. The study employs a custom-designed apparatus and readily available generic chemicals to perform the plastination process. The plastination procedure consists of four key stages, with the impregnation phase being carried out in a vacuum chamber utilizing a passive vacuum system. The resulting plastinated specimens were assessed for flexibility, color, and odor. The findings revealed that the plastinated organs exhibited a rigid texture, a pale coloration, and a mild odor. The anticipated outcome of this research is to provide actionable recommendations for improving plastination methods, thereby enhancing the quality of plastinated organ products for use in veterinary anatomy education.
HISTOLOGICAL STRUCTURE AND HISTOMORPHOMETRY OF THE BALI DUCK OESOPHAGUS IN STARTER PHASE Eunike Beallara Hutapea; Ni Luh Eka Setiasih; Desak Nyoman Dewi Indira Laksmi; Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p25

Abstract

Bali duck (Anas sp.) is a native Indonesian duck breed developed in the Bali and Lombok regions. Bali duck have significant potential as producers of eggs and meat, contributing to the fulfilment of animal protein needs of the Indonesian population. This study was conducted to determine the histological structure and histomorphometry of the oesophagus of bali duck during the starter phase. Samples were taken from ducks aged 1 day, 14 days, 28 days, 42 days, and 56 days. Histological sample observations focused on the layers composing the oesophagus. Parameters used for histomorphometry measurements were the thickness of the oesophageal tunica layers and he results of the research on histological structure are presented in a descriptive qualitative form. The results of the research on histomorphometry are presented in a descriptive quantitative form, analysed using Analysis of Variance (ANOVA) and if the results showed a significant difference, were followed by Duncan’s post-hoc test. The study results indicated that histologically, the bali duck oesophagus consists of the tunica mucosa, tunica submucosa, tunica muscularis, and tunica adventitia. Histomorphometry results showed an increase in the thickness of each layer with increasing age, from 1 day, 14 days, 28 days, 42 days, to 56 days. Further studies in different phase employing special staining techniques are required to allow for more detailed identification.
HISTOLOGICAL STRUCTURE AND HISTOMORPHOMETRY OF THE BALI DUCK PROVENTRICULUS IN STARTER PHASE Ditha Amelia Himelda Putri; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Ketut Berata; Luh Gde Sri Surya Heryani; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p17

Abstract

The proventriculus is one of the primary digestive organs in poultry, functioning as a gland responsible for enzymatic digestion through the secretion of hydrochloric acid and pepsinogen. This study aimed to examine the histological structure and histomorphometry of the proventriculus in Bali ducks during the starter phase. Samples were collected from ducks aged 1, 14, 28, 42, and 56 days, totaling 30 individuals (15 males and 15 females). Histological observations were performed using a binocular microscope, while histomorphometric measurements were conducted using the ImageJ application. Histological data were presented descriptively, and histomorphometric data were analyzed using Analysis of Variance (ANOVA) followed by Duncan’s test. The histological structure of the proventriculus consists of the tunica mucosa, tunica submucosa, tunica muscularis, and tunica serosa. All four layers showed increased thickness with advancing age: the tunica mucosa increased from 156.31 µm to 352.31 µm, the tunica submucosa from 1,113.71 µm to 2,270.87 µm, the tunica muscularis from 215.62 µm to 573.62 µm, and the tunica serosa from 132.86 µm to 486.70 µm. No significant differences (p>0.05) were found in the histological structure between male and female ducks. However, significant differences (p<0.05) in histomorphometric measurements were observed among different age groups.
MORPHOMETRY AND HISTOLOGY STRUCTURE OF CEREBELLUM OF BALI DUCKS Sekar Aurellya Savira; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p08

Abstract

The cerebellum, located in the dorsal region of the medulla oblongata and pons, is the largest component of the hindbrain. It plays a crucial role in motor coordination, nerve control, and maintaining body balance. This study aimed to analyze the anatomical and histological structure, as well as the morphometry, of the cerebellum in male and female growing-phase Bali ducks. A total of 20 Bali ducks (10 males and 10 females, aged 3 months each) were used in this study. The cerebellum was extracted for anatomical observation and weight measurement. Subsequently, the samples were processed into histological preparations using Hematoxylin-Eosin (HE) staining to examine the histological structure and morphometric parameters. Anatomically, the cerebellum was positioned at the posterior region of the head, beneath the temporal and occipital lobes and above the brainstem. The average cerebellar weight was 0.6 grams in males and 0.5 grams in females. Histologically, the cerebellar structure was consistent across both sexes, comprising gray matter (molecular layer, granular layer, and Purkinje/ganglionar layer) and white matter. Morphometric analysis revealed the following mean layer thicknesses in males: molecular layer (156.29 ± 17.72 µm), granular layer (69.73 ± 11.26 µm), Purkinje layer (6.44 ± 1.68 µm), and white matter (50.11 ± 19.16 µm). In females, the corresponding values were 145.47 ± 15.07 µm, 66.83 ± 11.37 µm, 6.83 ± 1.64 µm, and 52.89 ± 18.56 µm, respectively. Statistical analysis indicated no significant differences (P > 0.05) in cerebellar weight or layer thickness between male and female Bali ducks. Further research with a larger sample size and additional parameters, such as biochemical or functional studies, could provide deeper insights into sex-related variations in cerebellar characteristics.
Co-Authors Desak Nyoman Dewi Indira Laksmi Dionysius Immanuel Ditha Amelia Himelda Putri Eunike Beallara Hutapea I Ketut Berata I Ketut Suatha I Nengah Wandia Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Sekar Aurellya Savira Sri Kayati Widyastuti