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All Journal Buletin Veteriner Udayana
Ni Luh Eka Setiasih
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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HISTOLOGICAL STRUCTURE OF BALI DUCKS SKIN IN GROWER PHASE Dionysius Immanuel; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Ketut Berata; Luh Gde Sri Surya Heryani; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p07

Abstract

The skin is the largest and outermost organ that can be directly observed. The skin plays a crucial role for bali ducks as a protective barrier against the environment, maintaining body temperature, retaining body fluids, and neurosensory functions. This study aims to determine the structure of the bali duck skin (Anas sp.) during the grower phase. This study used ten bali ducks aged 3 months. The histological structure was observed using a binocular light microscope. The histological structure was analyzed descriptively and qualitatively. The structure of bali duck skin consists of three layers: epidermis, dermis, and hypodermis. The epidermis has three layers: stratum corneum, stratum intermedium, and stratum basale. The dermis has two layers: stratum compactum and stratum laxum. The tissues found in the bali duck skin include feather follicles, blood vessels, Herbst corpuscles, connective tissue, and musculus erector pili. Based on the research conducted, no differences in the histological structure of the skin were found across the three regions. Further research is needed on the skin structure of Bali ducks at different phases and sexes to obtain more comprehensive data, and specific histological studies are required to identify the skin histology of Bali ducks in greater detail.
EFFECT OF PHYLLANTHUS NIRURI LEAF EXTRACT AT DIFFERENT DOSES ON THE ANTIBODY TITER RESPONSE TO NEWCASTLE DISEASE IN BROILER I Putu Aditya Prawira; Ida Bagus Kade Suardana; I Made Kardena; Ni Luh Eka Setiasih; Tjokorda Sari Nindhia; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p25

Abstract

Newcastle Disease (ND), also known as tetelo, is one of the major poultry diseases that causes significant economic losses in the poultry industry due to decreased productivity and increased mortality rates in broiler chickens. This study aimed to evaluate the effect of Phyllanthus niruri Linn (green meniran) extract at different oral doses on ND antibody titers in broiler chickens. This study employed an experimental method with a completely randomized design, involving 30 broiler chickens divided into three treatment groups: a control group (without extract), a group receiving Phyllanthus niruri extract at a dose of 7 mg/kgW/day, and a group receiving 9 mg/kgW/day for four weeks. ND antibody titers were analyzed using the Hemagglutination Inhibition (HI) test. The results showed that the administration of Phyllanthus niruri extract significantly increased ND antibody titers, with the highest immune response observed in the group receiving 9 mg/kg W/day compared to the 7 mg/kg W/day and control groups. The increase in antibody titers was noticeable from the first week post-vaccination and peaked in the second week. These findings suggest that Phyllanthus niruri extract has potential as a natural immunostimulant to enhance immune responses against ND in broiler chickens. Further studies are needed to evaluate higher doses and the long-term effects of Phyllanthus niruri extract administration on the health and productivity of broiler chickens.
HISTOLOGICAL STRUCTURE OF LYMPHOCYTES, MONOCYTES IN DOGS WITH DERMATITIS PRE AND POST THERAPY Made Ananda Giri Tanaya; Ni Ketut Suwiti; I Nyoman Suartha; Sri Kayati Widyastuti; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p22

Abstract

A study was conducted to analyse the histological structure of lymphocytes and monocytes in dogs with dermatitis before and after therapy. Dermatitis is skin inflammation that can be caused by bacterial infections, allergies, or environmental factors. This study used 20 dogs with dermatitis that were given a combination of antiparasitic, antibiotic, anti-inflammatory, and antihistamine therapy. Blood samples were taken before and after therapy to be analysed using Morphology of Edge Blood (MDT) staining to observe changes in the histological structure of lymphocyte and monocyte cells. The results showed: before therapy, lymphocytes and monocytes experienced more necrosis (P<0.05) histological structure changes such as pycnosis, karyorexis, and karyolysis. After therapy, there was a decrease in the number of cells that experienced necrosis in lymphocytes, from 8.45 ± 2.08% to 6.85 ± 1.75%. Whereas in monocytes, there was a decrease in the number (P<0.05) of necrotised cells at post- therapy, from 27.5±19.4% to 13.5±10.3%. The conclusion of this study is that the histological structure of lymphocytes and monocytes that experience necrosis is less than cells that do not necrose. The histological structure of the cells showed a lot more normal. The combination therapy used proved effective in improving the structure of lymphocytes and monocytes.
HISTOPATHOLOGICAL ANALYSIS OF INCISION WOUND HEALING IN RATS GIVEN PLATELET RICH PLASMA DROPS FROM PIG BLOOD Anak Agung Ngurah Hery Brian Priantara; I Wayan Wirata; I Ketut Berata; I Gusti Ngurah Sudisma; I Wayan Gorda Sudisma; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p09

Abstract

Platelet Rich Plasma (PRP) is platelet-rich plasma derived from whole blood. Platelet Rich Plasma (PRP) contains growth factors such as transforming growth factor-beta (TGF-β), insulin like growth factor (IGF-I), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and epidermal growth factor (EGF) which play a role in accelerating wound healing. This study aims to determine the histopathological analysis of incision wound healing of white rats given pig blood PRP drops. This study used white rats of male sex with the age of 2 - 2.5 months with a body weight of 200 - 300 g. The rats used in this study were 27 rats divided into two groups. The 27 rats used were divided into 3 treatment groups, namely P0 (negative control, given 0.9% NaCl solution), P1 (positive control, given Bioplacenton), P2 (given PRP drops). On days 1, 5, and 11, biopsies were taken for histopathological examination. Histopathological examination was performed including four indicators: inflammatory cell infiltration, angiogenesis, fibroblasts, and collagen density. Data were then analyzed using Kruskal-Wallis and if there was a significant difference (P<0.05), it would be followed by the Mann Whitney test. From the results of the study on the histopathology of white rat incision wounds given pig blood PRP drops showed an increase in development. On the first day, inflammatory cell infiltration and angiogenesis increased, but decreased on days 5 and 11. Fibroblasts were seen on the first day then decreased until day 11. Collagen on day 1 began to be seen until on day 11 the density of collagen was very tight. It is necessary to make observations in a more detailed period of time to find out more clearly about the effect of PRP drops on angiogenesis.
THE EFFECT OF MENIRAN EXTRACT AS AN IMUNOSTIMULATOR ON THE INCREASE OF NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS Muhammad Evan Malik; Ida Bagus Kade Suardana; Samsuri; Ni Luh Eka Setiasih; I Made Kardena; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p11

Abstract

Meniran is known as an herbal plant with potential as a natural immunostimulator due to its content of flavonoids, saponins, and tannins. These compounds help boost the immune response by stimulating cytokine production, accelerating immune cell activation, and increasing antibody production. Newcastle Disease (ND) is an infectious disease that frequently affects broilers and causes significant economic losses. Vaccination is the primary method for preventing ND; however, its effectiveness can vary depending on the immune response of the chickens. This study aims to evaluate the effect of meniran extract as an immunostimulant on increasing Newcastle Disease antibody titers in booster-vaccinated broilers. This study used a Completely Randomized Design with 30 Lohman MB 202 broilers divided into three treatment groups. The negative control group (K-) received neither vaccination nor meniran extract, the positive control group (K+) received only the ND La Sota booster vaccine, and the treatment group (P) received both the ND La Sota booster vaccine and meniran extract for 14 days before vaccination. Antibody titers were measured using the Hemagglutination Inhibition (HI) test on days 17, 25, and 32. Data were analyzed using ANOVA and regression analysis. The results showed that the administration of meniran extract significantly increased antibody titers (P<0.05), with mean values of 2.7, 4.1, and 6.0 (HI log 2), and an overall mean of 4.27. Regression analysis indicated that the treatment group (P) experienced a higher increase in antibody titers compared to the control groups, with the peak immune response occurring in the third week post-vaccination. The conclusion of this study is that meniran extract can act as a natural immunostimulant that enhances the effectiveness of the ND vaccine in broiler chickens. Therefore, further research with different doses and administration durations is needed to optimize its benefits.
MORPHOMETRY AND IDENTIFICATION OF HISTOLOGICAL CHARACTERISTICS OF BALI CATTLE OVARIES Ni Putu Amasya Chikita Rivanny; Tjok Gde Oka Pemayun; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p17

Abstract

Bali cattle (Bos sondaicus) are said to be fertile because the right and left ovaries of Bali cattle have the same opportunity in their reproductive activity. Ovaries activity can be reviewed morphometrically and histologically to determine the characteristics of each estrus phase. This study was conducted with the aim of determining the differences in morphometry of the right and left ovaries in Bali cattle, as well as identifying the histological features of the ovaries of Bali cattle. The morphometry study of the right and left ovaries was conducted descriptively quantitatively where the data was processed statistically with a paired sample T- test. Identification of histological characteristics was conducted descriptively qualitatively. The results of the morphometry calculations stated that the weight and width of the right and left ovaries of Bali cattle were not significantly different (P>0.05), while the length of the ovaries was significantly different (P<0.05). The results of histological identification can be identified through the development of follicles and corpus luteum to become corpus albicans.
HISTOLOGICAL STRUCTURE AND HISTOMORPHOMETRY OF THE BALI DUCK OESOPHAGUS IN STARTER PHASE Eunike Beallara Hutapea; Ni Luh Eka Setiasih; Desak Nyoman Dewi Indira Laksmi; Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p25

Abstract

Bali duck (Anas sp.) is a native Indonesian duck breed developed in the Bali and Lombok regions. Bali duck have significant potential as producers of eggs and meat, contributing to the fulfilment of animal protein needs of the Indonesian population. This study was conducted to determine the histological structure and histomorphometry of the oesophagus of bali duck during the starter phase. Samples were taken from ducks aged 1 day, 14 days, 28 days, 42 days, and 56 days. Histological sample observations focused on the layers composing the oesophagus. Parameters used for histomorphometry measurements were the thickness of the oesophageal tunica layers and he results of the research on histological structure are presented in a descriptive qualitative form. The results of the research on histomorphometry are presented in a descriptive quantitative form, analysed using Analysis of Variance (ANOVA) and if the results showed a significant difference, were followed by Duncan’s post-hoc test. The study results indicated that histologically, the bali duck oesophagus consists of the tunica mucosa, tunica submucosa, tunica muscularis, and tunica adventitia. Histomorphometry results showed an increase in the thickness of each layer with increasing age, from 1 day, 14 days, 28 days, 42 days, to 56 days. Further studies in different phase employing special staining techniques are required to allow for more detailed identification.
HISTOLOGICAL AND HISTOMORPHOMETRIC ANALYSIS OF THE VENTRICULUS IN BALINESE DUCKS DURING THE STARTER PHASE Ana Angelica Floarta Nasa; Ni Luh Eka Setiasih; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p38

Abstract

Bali ducks are a source of wealth and genetic resources originating from Bali. This study aims to determine the histological and histomorphometric structure of the ventriculus of bali ducks in the starter phase. The samples used consisted of 15 male Bali ducks and 15 female bali ducks aged 1 day, 14 days, 28 days, 42 days, and 56 days. The histological structure was examined using a binocular light microscope, and histomorphometry was measured using the ImageJ application and analyzed with the assistance of SPSS software. The results of this study showed that the histological structure of the bali duck ventriculus consists of the cuticle, mucosa, submucosa, and muscularis. The histomorphometric results indicated that the thickness of the cuticle increased from 153.884 μm to 387.559 μm, the mucosa increased from 420.448 μm to 779.638 μm, the submucosa increased from 56.634 μm to 260.631 μm, and the muscularis increased from 1010.344 μm to 2420.951 μm. The results of the study indicate that there are no differences in anatomical and histological structure in the bali duck ventricle. However, there are histomorphometric differences in the bali duck ventricle at 1 day, 14 days, 28 days, 42 days, and 56 days of age.
HISTOLOGICAL STRUCTURE AND HISTOMORPHOMETRY OF THE BALI DUCK PROVENTRICULUS IN STARTER PHASE Ditha Amelia Himelda Putri; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Ketut Berata; Luh Gde Sri Surya Heryani; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p17

Abstract

The proventriculus is one of the primary digestive organs in poultry, functioning as a gland responsible for enzymatic digestion through the secretion of hydrochloric acid and pepsinogen. This study aimed to examine the histological structure and histomorphometry of the proventriculus in Bali ducks during the starter phase. Samples were collected from ducks aged 1, 14, 28, 42, and 56 days, totaling 30 individuals (15 males and 15 females). Histological observations were performed using a binocular microscope, while histomorphometric measurements were conducted using the ImageJ application. Histological data were presented descriptively, and histomorphometric data were analyzed using Analysis of Variance (ANOVA) followed by Duncan’s test. The histological structure of the proventriculus consists of the tunica mucosa, tunica submucosa, tunica muscularis, and tunica serosa. All four layers showed increased thickness with advancing age: the tunica mucosa increased from 156.31 µm to 352.31 µm, the tunica submucosa from 1,113.71 µm to 2,270.87 µm, the tunica muscularis from 215.62 µm to 573.62 µm, and the tunica serosa from 132.86 µm to 486.70 µm. No significant differences (p>0.05) were found in the histological structure between male and female ducks. However, significant differences (p<0.05) in histomorphometric measurements were observed among different age groups.
MORPHOMETRY AND HISTOLOGY STRUCTURE OF CEREBELLUM OF BALI DUCKS Sekar Aurellya Savira; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p08

Abstract

The cerebellum, located in the dorsal region of the medulla oblongata and pons, is the largest component of the hindbrain. It plays a crucial role in motor coordination, nerve control, and maintaining body balance. This study aimed to analyze the anatomical and histological structure, as well as the morphometry, of the cerebellum in male and female growing-phase Bali ducks. A total of 20 Bali ducks (10 males and 10 females, aged 3 months each) were used in this study. The cerebellum was extracted for anatomical observation and weight measurement. Subsequently, the samples were processed into histological preparations using Hematoxylin-Eosin (HE) staining to examine the histological structure and morphometric parameters. Anatomically, the cerebellum was positioned at the posterior region of the head, beneath the temporal and occipital lobes and above the brainstem. The average cerebellar weight was 0.6 grams in males and 0.5 grams in females. Histologically, the cerebellar structure was consistent across both sexes, comprising gray matter (molecular layer, granular layer, and Purkinje/ganglionar layer) and white matter. Morphometric analysis revealed the following mean layer thicknesses in males: molecular layer (156.29 ± 17.72 µm), granular layer (69.73 ± 11.26 µm), Purkinje layer (6.44 ± 1.68 µm), and white matter (50.11 ± 19.16 µm). In females, the corresponding values were 145.47 ± 15.07 µm, 66.83 ± 11.37 µm, 6.83 ± 1.64 µm, and 52.89 ± 18.56 µm, respectively. Statistical analysis indicated no significant differences (P > 0.05) in cerebellar weight or layer thickness between male and female Bali ducks. Further research with a larger sample size and additional parameters, such as biochemical or functional studies, could provide deeper insights into sex-related variations in cerebellar characteristics.
Co-Authors Ana Angelica Floarta Nasa Anak Agung Ngurah Hery Brian Priantara Anak Agung Sagung Kendran Desak Nyoman Dewi Indira Laksmi Dionysius Immanuel Ditha Amelia Himelda Putri Eunike Beallara Hutapea I Gusti Ngurah Sudisma I Ketut Berata I Made Kardena I Nyoman Suartha I Putu Aditya Prawira I Wayan Gorda Sudisma I Wayan Wirata Ida Bagus Kade Suardana Luh Gde Sri Surya Heryani Made Ananda Giri Tanaya Muhammad Evan Malik Ni Ketut Suwiti Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Putu Amasya Chikita Rivanny Samsuri Sekar Aurellya Savira Sri Kayati Widyastuti Tjok Gde Oka Pemayun Tjokorda Sari Nindhia