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All Journal Buletin Veteriner Udayana
Gusti Ayu Yuniati Kencana
Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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TOTAL AND DIFFERENTIAL LEUKOCYTES OF LAYING CHICKENS AFTER NEWCASTLE DISEASE AND INFECTIOUS BRONCHITIS VACCINATION Iqbal Bayu Samudra; Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana; I Made Damriyasa; Ida Bagus Kade Suardana; I Gede Soma
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p41

Abstract

Research on the total and differential leukocytes of post-vaccination laying hens which was carried out on commercial farms in Tabanan Regency, Bali aims to determine and study the effect of combination vaccines Newcastle Disease LaSota strains and Infectious Bronchitis on the total number and types of leukocytes in the blood. A total of 40 laying hens aged 30 weeks were taken using purposive sampling for the research sample. Chicken blood samples were taken four times, starting from pre-vaccination to three weeks post-vaccination. The total leukocyte count is checked using a hemocytometer, while the leukocyte differential is checked using a blood smear. Data from total and differential leukocyte examination results were analyzed quantitatively descriptively. The results showed that there was a significant effect (p<0.05) on total leukocytes, heterophils, eosinophils and lymphocytes, and no significant effect (p>0.05) on basophils and monocytes.
CASE OF COCCIDIOSIS IN BROILER CHICKENS AT A FARM IN BENOA VILLAGE, BADUNG REGENCY Ni Putu Juni Ratna Dewi; I Made Dwinata; I Made Kardena; I Gusti Ketut Suarjana; Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p16

Abstract

The subject of this case study is a 28-day-old broiler chicken from a farm located in Benoa, South Kuta District, Bali. The chicken was reported to exhibit clinical symptoms such as weakness, isolation from the flock, loss of appetite, and bloody feces. This study aims to analyze the anatomical pathology, histopathology, and laboratory findings to establish a definitive diagnosis of the disease affecting the chicken. Data collection included signalment, anamnesis, epidemiological investigation, and parasitic examination, which were then analyzed qualitatively in a descriptive manner. Necropsy findings after the chicken's death revealed congestion and hemorrhage in the small intestine, as well as bleeding with distension in the cecum. Histopathological observations showed necrosis of the cecal mucosa, the presence of schizonts, inflammatory cell infiltration, and the identification of macrogametes and microgametes. Qualitative fecal examination identified schizonts of Eimeria spp. Based on clinical, pathological, and laboratory analyses, it was concluded that the chicken suffered from coccidiosis.
PREVALENCE COMPARISON OF NEWCASTLE DISEASE VIRUS CASES AT CHICKEN FARMS IN BLAHBATUH AND PAYANGAN SUBDISTRICTS, GIANYAR DISTRICT Ingrid Laurentina; Gusti Ayu Yuniati Kencana; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p07

Abstract

Newcastle Disease (ND) is a common poultry disease in Indonesia, caused by Avian Paramyxovirus serotype 1 (APMV-1). This disease is endemic and occurs throughout the year affecting various types of poultry, including commercial and backyard chickens. This study aims to compare the prevalence of the ND virus in poultry farms in Blahbatuh and Payangan Districts, Gianyar Regency. This study employed an observational method with a cross-sectional approach. Chickens showing clinical symptoms were subjected to necropsy to collect tissue samples that exhibited pathological changes. These samples were inoculated into Specific Pathogen-Free (SPF) embryonated chicken eggs for ND virus growth. After incubation, the allantoic fluid was harvested and tested using HA and HI. A positive HA test result was indicated by the formation of sand like sediment, while a positive HI test result showed a 1% erythrocyte deposit forming a dot at the bottom of the microplate well. The study results indicated that 9 out of 32 chickens (28%) tested positive for ND. Chickens sampled in Payangan Subdistrict were found to have a 1.36 times higher risk of infection compared to those sampled in Blahbatuh subdistrict. Increasing farmers awareness of the importance of regular ND vaccination and improving biosecurity like isolation cage systems is crucial to preventing the spread of the disease.
COLISEPTICEMIA IN BROILER CHICKEN FARM IN BATUNGSEL VILLAGE, PUPUAN DISTRICT, TABANAN DISTRICT, BALI Anak Agung Sagung Massita Jenika Putri; I Gusti Ketut Suarjana; Ida Bagus Made Oka; I Ketut Berata; Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p34

Abstract

Escherichia coli is the most common bacterial infection found in broiler chicken farms. Escherichia coli infection in poultry is generally systemic and causes bacteremia, so it is called colispticemia. Case reports were conducted to diagnose animals with protocol number 116/N/25 based on anamnesis data, clinical signs, epidemiology, anatomical pathology changes, and histopathological changes and examination in the bacteriology laboratory. 30-day-old white broiler chickens were taken from a farm owned by Mr. Rudi located in Batungsel Village, Pupuan District, Tabanan Regency. The clinical signs observed were that the chickens showed abdominal distension, were weak and did not want to eat, had slow growth compared to other chickens, diarrhea, dirt around the cloaca, shortness of breath and weight loss. After the case chicken died, a necropsy was performed, then organ samples were taken and fixed with 10% Neutral Buffered Formalin (NBF). Pathological examination of the anatomy found a layer of fibrin in the heart, liver and lungs. Tissue samples taken were brain, trachea, lungs, liver, heart, spleen, kidneys and intestines, then histopathological preparations were made with hematoxylin-eosin staining. The results of histopathological examination found infiltration of heterophilic inflammatory cells in the lungs, liver, kidneys and intestines, indicating that the case animals were infected with bacteria. Examination of samples in the bacteriology and mycology laboratories obtained positive results for Escherichia coli bacteria in heart, lung, liver and intestine specimens. It can be concluded that the case chickens were infected by E. coli bacteria or were attacked by Colisepticemia. There needs to be increased sanitation and strict biosecurity in chicken farms
Co-Authors Anak Agung Sagung Kendran Anak Agung Sagung Massita Jenika Putri I Gede Soma I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Ketut Berata I Made Damriyasa I Made Dwinata I Made Kardena Ida Bagus Kade Suardana Ida Bagus Made Oka Ingrid Laurentina Iqbal Bayu Samudra Ni Putu Juni Ratna Dewi