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All Journal Buletin Veteriner Udayana
Anak Agung Sagung Kendran
Laboratorium Patologi Klinik Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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THE EFFECT OF GIVING GAMAL LEAF BLOCK ON THE HEMATOLOGICAL PROFILE OF CATTLE EXPERIENCING ANESTRUS Resinta Delia Rahmadani; Samsuri; I Nengah Wandia; I Made Merdana; I Nyoman Sulabda; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p14

Abstract

Anestrus is a condition in female animals that do not show symptoms of estrus for a long period of time. Anestrus in cows can cause physiological, environmental and management changes. Gamal leaves can be used as a source of protein for livestock. Mother cows experience fluctuating hematological changes, such as hemoglobinemia, erythropenia and leucocythemia. This study aims to determine the effect of gamal block on hemoglobin levels, total erythrocytes, hematocrit values ​​and total leukocytes in Bali cattle experiencing anestrus. This research used 6 anestrus Bali cattle selected using purposive sampling. The six cows were given gamal block treatment with a composition of 250 grams of gamal leaf simplicial, 200 ml propylene glycol and 4 grams of salt for 14 days. After that, a 2ml blood sample was taken via the jugular vein on day 0, day 10 and day 20. The results of the study obtained mean hemoglobin levels sequentially on days 0, 10 and 20, namely 8.98 ± 0.23 g/dL, 8.78 ± 0.23 g/dL, 8.26 ± 0, 23 g/dL. Total erythrocytes were 4.80±0.18 106/μL, 4.92±0.18 106/μL, 4.73±0.18106/μL respectively. Hematocrit values ​​were 29.53 ± 0.60 %, 28.23 ± 0.60 %, 26.64 ± 0.61 %, respectively. And total leukocytes were 7.21±0.27 103/μL, 7.56±0.27 103/μL, 6.63±0.27 103/μL respectively. The statistical results showed that giving gamal leaf blocks for 14 days did not affect hemoglobin levels, total erythrocytes, hematocrit values ​​and total leukocytes in Bali cattle experiencing anestrus.
EFFECT OF PHYLLANTHUS NIRURI LEAF EXTRACT AT DIFFERENT DOSES ON THE ANTIBODY TITER RESPONSE TO NEWCASTLE DISEASE IN BROILER I Putu Aditya Prawira; Ida Bagus Kade Suardana; I Made Kardena; Ni Luh Eka Setiasih; Tjokorda Sari Nindhia; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p25

Abstract

Newcastle Disease (ND), also known as tetelo, is one of the major poultry diseases that causes significant economic losses in the poultry industry due to decreased productivity and increased mortality rates in broiler chickens. This study aimed to evaluate the effect of Phyllanthus niruri Linn (green meniran) extract at different oral doses on ND antibody titers in broiler chickens. This study employed an experimental method with a completely randomized design, involving 30 broiler chickens divided into three treatment groups: a control group (without extract), a group receiving Phyllanthus niruri extract at a dose of 7 mg/kgW/day, and a group receiving 9 mg/kgW/day for four weeks. ND antibody titers were analyzed using the Hemagglutination Inhibition (HI) test. The results showed that the administration of Phyllanthus niruri extract significantly increased ND antibody titers, with the highest immune response observed in the group receiving 9 mg/kg W/day compared to the 7 mg/kg W/day and control groups. The increase in antibody titers was noticeable from the first week post-vaccination and peaked in the second week. These findings suggest that Phyllanthus niruri extract has potential as a natural immunostimulant to enhance immune responses against ND in broiler chickens. Further studies are needed to evaluate higher doses and the long-term effects of Phyllanthus niruri extract administration on the health and productivity of broiler chickens.
TOTAL AND DIFFERENTIAL LEUKOCYTES OF LAYING CHICKENS AFTER NEWCASTLE DISEASE AND INFECTIOUS BRONCHITIS VACCINATION Iqbal Bayu Samudra; Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana; I Made Damriyasa; Ida Bagus Kade Suardana; I Gede Soma
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p41

Abstract

Research on the total and differential leukocytes of post-vaccination laying hens which was carried out on commercial farms in Tabanan Regency, Bali aims to determine and study the effect of combination vaccines Newcastle Disease LaSota strains and Infectious Bronchitis on the total number and types of leukocytes in the blood. A total of 40 laying hens aged 30 weeks were taken using purposive sampling for the research sample. Chicken blood samples were taken four times, starting from pre-vaccination to three weeks post-vaccination. The total leukocyte count is checked using a hemocytometer, while the leukocyte differential is checked using a blood smear. Data from total and differential leukocyte examination results were analyzed quantitatively descriptively. The results showed that there was a significant effect (p<0.05) on total leukocytes, heterophils, eosinophils and lymphocytes, and no significant effect (p>0.05) on basophils and monocytes.
THE EFFECTS OF TRANSFORMATION DURATION ON THE QUANTITY OF SWINE PATHOGENIC ESCHERICHIA COLI ISOLATE Kalyana Lionita; I Gusti Ngurah Kade Mahardika; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p04

Abstract

DNA transformation generally still utilizes commercial Escherichia coli bacteria, and the use of virulent pathogenic bacteria has never been reported. Utilizing virulent pathogenic bacteria can provide an advantage, as the product can be used as a vaccine against the genetic material in the plasmid and against the pathogenic bacteria itself. This study aims to determine how the transformation duration affects the number of swine Escherichia coli pathogenic isolates colonies. The samples of this study are swine Escherichia coli pathogenic isolates BPOS4 and E. coli BL21 (DE3) which were made into competent cells by calcium chloride heat shock method and then transformed with the different transformation of times using plasmid pGEX-2T+ASF A224L. The results showed that E. coli BL21 (DE3) and swine Escherichia coli pathogenic isolates BPOS4 had no significant effect on the quantity of colonies after transformation. Although statistically the effect was not significant, E. coli BL21(DE3) gave a higher colony count with an average of 62,4×105 CFU/100µL compared to E. coli BPOS4 of 33,45×105 CFU/100µL. The transformation time has no significant effect on the number of bacterial colonies after transformation, however, the best transformation time is shown by the treatment of 60 seconds with an average colony of 86,5×105 CFU/100µL. It can be concluded that the colonies of E. coli BL21 (DE3) and E. coli BPOS4 transformed with plasmid pGEX-2T+ASF A224L can grow optimally in 60 seconds. Further research can be conducted, such as developing Escherichia coli vaccines using pathogenic strains.
THE IMMUNOMODULATORY EFFECT OF PHYLLANTHUS NIRURI L. EXTRACT ON ANTIBODY TITERS IN NEWCASTLE DISEASE-VACCINATED BROILERS Daniel Halim; Ida Bagus Kade Suardana; Tjokorda Sari Nindhia; Tri Komala Sari; Ni Luh Eka Setiasih; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2025 Vol. 17 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i06.p06

Abstract

Newcastle Disease (ND) remains a major threat to the poultry industry, and while vaccination is essential, the immune response can be inconsistent. This study evaluated the potential of Phyllanthus niruri L. (meniran) extract as an immunomodulator to enhance the humoral immune response in broilers post-ND vaccination. A total of 30 one-day-old broilers, vaccinated against ND, were divided into three treatment groups: P0 (control, no extract), P1 (extract at 5 mg/kg BW/day via drinking water from day 3 to 17), and P2 (extract from day 3 to 24). Antibody titers were measured using the Hemagglutination Inhibition (HI) test and analysed with ANOVA using SPSS software. The results demonstrated that meniran extract significantly increased antibody titters compared to the control (P<0.05). Group P2, which received the extract until day 24, achieved the highest mean titter (6.1 log2 HI units), followed by P1 (4.4 log2) and the control P0 (4.0 log2). Data analysis confirmed a positive relationship between the duration of extract administration and antibody levels. In conclusion, Phyllanthus niruri L. extract is an effective immunomodulator that significantly enhances the ND vaccine-induced antibody response in broilers, with a longer administration period yielding a more robust effect.
Co-Authors Daniel Halim Gusti Ayu Yuniati Kencana I Gede Soma I Gusti Ngurah Kade Mahardika I Made Damriyasa I Made Kardena I Made Merdana I Nengah Wandia I Nyoman Sulabda I Putu Aditya Prawira Ida Bagus Kade Suardana Ida Bagus Kade Suardana Iqbal Bayu Samudra Kalyana Lionita Ni Luh Eka Setiasih Ni Luh Eka Setiasih Resinta Delia Rahmadani Samsuri Tjokorda Sari Nindhia Tri Komala Sari