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All Journal Advances in Tropical Biodiversity and Environmental Sciences
I Wayan Gorda
Udayana University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Phytochemical Test and Antioxidant Activity of Aqueous Extract of Marigold Flower I Made Merdana; Indira Laksmi Nandita; Pingkan Elia; Ida Ayu Pasti Apsari; I Wayan Gorda; Luh Made Sudimartini
Advances in Tropical Biodiversity and Environmental Sciences Vol. 8 No. 2 (2024): June 2024: Pages 58-118
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2024.v08.i02.p08

Abstract

Natural medicines are increasingly in demand for safety reasons and are believed to be able to cure certain diseases. Literacy reveals that the marigold flower (Tagetes erecta L.) has medicinal potential as an antioxidant, anti-bacterial, anti-inflammatory, and anti-carcinogen. This research aimed to identify marigold flower aqueous extract's phytochemical content and antioxidant activity. The method used in this exploratory research was the maceration of marigold flower simplicia using distilled water for 24 hours, followed by evaporation to obtain marigold flower aqueous extract. Then, phytochemical screening and antioxidant bioactivity tests were carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results of phytochemical screening showed that marigold flower water extract contained flavonoids, phenols, alkaloids, and tannins. Meanwhile, the antioxidant activity test obtained an IC50 value of 50.77 ppm. From these results, it can be concluded that the aqueous extract of marigold flowers has medicinal potential with vigorous antioxidant activity.
Effect of Palmyra Fruit Water–Egg Yolk Diluent with Moringa Leaf Extract on Boar Sperm Membrane Integrity and Motility I Wayan Gorda; I Putu Gde Pradnya Nolan Toli; Wayan Bebas
Advances in Tropical Biodiversity and Environmental Sciences Vol. 10 No. 1 (2026): February 2026
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2026.v010.i01.p02

Abstract

This study aimed to evaluate the effect of palmyra fruit water–egg yolk diluents supplemented with moringa (Moringa oleifera L.) leaf extract on the intact plasma membrane, intact acrosomal membrane, and motility of boar spermatozoa during liquid storage. A completely randomized design was used, with seven diluent formulations varying in concentrations of egg yolk and moringa leaf extract. Each treatment was replicated four times and stored at 15-18°C for up to 72 hours. Semen quality was evaluated at 0, 24, 48, and 72 hours of storage. Data were analyzed using analysis of variance followed by Duncan’s multiple range test. Both diluent formulation and storage duration significantly influenced all semen quality parameters (p < 0.05). The diluent containing palmyra fruit water supplemented with 20% egg yolk and 15% moringa leaf extract produced the highest membrane integrity, motility, and viability with the lowest abnormality rate. The semen maintained acceptable quality for artificial insemination for up to 48 hours of storage. The superior performance of this formulation is biologically associated with the synergistic interaction between carbohydrate-derived energy from palmyra fruit water, phospholipid-mediated membrane stabilization from egg yolk, and antioxidant protection from moringa leaf extract against oxidative stress.
Co-Authors I Made Merdana I Putu Gde Pradnya Nolan Toli I Wayan Bebas Ida Ayu Pasti Apsari Indira Laksmi Nandita Luh Made Sudimartini Pingkan Elia