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All Journal Edumedia: Jurnal Keguruan dan Ilmu Pendidikan Advances in Tropical Biodiversity and Environmental Sciences
Anita Constanci Christine Tengker
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Identification of Bacteria Isolated from Sea Urchin (Diadema setosum) Gonads Inhibiting Pseudomonas fluorescens and Staphylococcus aureus Growth Andini Maurent Dwiyanti; Helen Joan Lawalata; Iriani Setyawati; Anita Constanci Christine Tengker; Nonny Manampiring
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 2 (2025): June 2025
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i02.p05

Abstract

Sea urchins are organisms that have antibacterial, antitumor, and anticancer abilities. The gonads can be used as a food source because they store 28 kinds of amino acids, vitamin B complex. This research aims to determine the type of bacteria from the gonads of sea urchins (Diadema setosum) which have inhibitory power against the growth of Pseudomonas fluorescens and Staphylococcus aureus bacteria. This research used an exploratory method which was analyzed descriptively, using stages in which sea urchin samples were taken and antibacterial tests against Pseudomonas fluorescens and Staphylococcus aureus from sea urchin gonads using the spread method. The media used for the process of isolating bacteria from the gonads of the sea urchin Diadema setosum is Nutrient Agar (NA) media. Isolate colonies that grew after incubation for 24 hours were separated based on differences in colony morphology. Morphological characterization of bacterial cells begins with gram staining. The diameter of the inhibition zone in the test bacteria shows different shapes. The research obtained 8 bacterial isolates, namely isolates B1-B8 which were gram-negative. The eight bacterial isolates have the potential as antibacterials against the test bacteria P. fluorescens and S. aureus. Isolates B1, B2, B3, B7 are similar to the genus Proteus, while isolates B4, B5, B6, and B8 are similar to the genus Citrobacter. The diameter of the largest inhibition zone in the P.fluorescens test bacteria, namely isolate B1, was 9.03 mm. Meanwhile, the smallest diameter for isolate B7 was 6.97 mm. In the S.aureus test bacteria, the largest diameter in isolate B1 was 11.09 mm. Meanwhile, the smallest diameter for isolate B4 was 8.57 mm.
Exopolysaccharides-Producing Lactic Acid Bacteria in Marinated Pakoba (Syzygium luzonense Merr.) Fruit Revanda Stevanie Hasani; Helen Joan Lawalata; Anita Constanci Christine Tengker; Marthy Stella Taulu; Fernando Andre Watung
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 3 (2025): October 2025
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i03.p06

Abstract

Pakoba fruit is one of the endemic flora of North Sulawesi which is rich in nutrients and has the potential to be fermented. The fermentation process occurs due to the bacteria in the fruit, including lactic acid bacteria (LAB). One of the interesting products from LAB fermentation is that it can produce secondary metabolites which have the potential to produce exopolysaccharides, namely high molecular weight and biodegradable polymers. This research aims to determine whether LAB can be obtained from fermentation of Pakoba marination and what types of LAB produce exopolysaccharides based on their phenotypic characteristics. This research uses a qualitative descriptive method to characterize the type of LAB and observe the exopolysaccharides produced by the bacteria. This research succeeded in isolating and identifying 10 LAB isolates based on their morphological and biochemical characters. Four isolates named MP(1)5.2, MP(1)7.1, MP(3)6.3, MP(3)7.2 can produce exopolysaccharides with a soft character but not mucus, where bacterial exopolysaccharides are believed to increase probiotics in the digestive tract. Genus level recognition (generic assignment) based on profile matching revealed that these four bacteria were from the genus Lactobacillus.
EFEKTIVITAS SEDIAAN GEL EKSTRAK DAUN PACAR AIR DAN LIDAH BUAYA TERHADAP PENYEMBUHAN LUKA SAYAT MENCIT Gabriela Tasya Paat; Iriani Setyawati; Meity Neltje Tanor; Helen Joan Lawalata; Anita Constanci Christine Tengker; Nonny Manampiring
Edumedia : Jurnal Keguruan dan Ilmu Pendidikan Vol 10 No 1 (2026): Edumedia: Jurnal Keguruan dan Ilmu Pendidikan
Publisher : Universitas Kapuas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51826/edumedia.v10i2.1906

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas kombinasi ekstrak daun pacar air (Impatiens balsamina, L.) dan lidah buaya (Aloe vera) dalam proses penyembuhan luka sayatan. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Hewan uji yang digunakan adalah mencit (Mus musculus) jantan dewasa yang dibagi dalam 5 kelompok, yaitu kontrol positif (F0, bioplacenton); kontrol negatif (F1, tanpa perlakuan); perlakuan formula sediaan gel dari ekstrak daun pacar air 2,5% dan lidah buaya 2,5% (F2); pacar air 5% dan lidah buaya 5% (F3); pacar air 10% dan lidah buaya 10% (F4). Bagian paha belakang kanan mencit yang sebelumnya telah dicukur dan diolesi alkohol 70% dibuat luka sayat dengan silet sepanjang 10 mm dan kedalaman 2 mm. Pemberian sediaan gel dilakukan setiap hari pada jam 10.00 WITA selama 12 hari. Hasil penyembuhan luka sayat yang paling tinggi ditunjukkan oleh kelompok F4 dan F3, di mana pada hari ke-8 luka sudah menutup sempurna. Hasil penelitian menunjukkan bahwa pemberian sediaan gel ekstrak daun tumbuhan pacar air 5% dan lidah buaya 5% lebih efektif dalam penyembuhan luka sayat daripada Bioplacenton.
Co-Authors Andini Maurent Dwiyanti Fernando Andre Watung Gabriela Tasya Paat Helen Joan Lawalata Iriani Setyawati Marthy Stella Taulu Meity Neltje Tanor Nonny Manampiring Revanda Stevanie Hasani