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Water-soluble nanomaterials self-assembly for improving the stability of natural food preservatives: A Review I Dewa Agung Panji Dwipayana
Jurnal Biologi Udayana Vol. 29 No. 2 (2025): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2025.v29.i02.p07

Food spoilage is still a global problem, contributing to foodborne illness, economic losses, and environmental burdens associated with food waste. Conventional chemical preservatives, while effective, face increasing regulatory restrictions and consumer concern regarding potential health risks, driving demand for safer and more natural alternatives. Natural preservatives such as essential oils, phenolic compounds, and antimicrobial peptides offer broad-spectrum antimicrobial and antioxidant activities but are limited by poor water solubility, volatility, degradation during processing, and inconsistent efficacy in complex food matrices. This narrative review examines recent advances in water-soluble self-assembled nanomaterials as stabilization and delivery systems for natural food preservatives. Emphasis is placed on supramolecular self-assembly principles, key non-covalent interactions in aqueous environments, common nanostructures including cyclodextrin inclusion complexes, polymer micelles, nanoemulsions, hydrogels, and vesicles, as well as assembly and characterization methods relevant to food applications. The review further discusses major food spoilage mechanisms and bacterial pathogens, highlighting synergistic effects achieved by combining nanomaterials with natural preservatives to enhance antimicrobial efficacy, prolong shelf life, and reduce sensory impacts. While these systems demonstrate significant promise for clean-label food preservation, challenges related to scalability, cost, sensory optimization, safety, migration, and regulatory acceptance remain. Addressing these issues through green synthesis, mechanistic studies, and robust safety assessments will be essential to support the responsible translation of self-assembled nanomaterials into practical and sustainable food preservation strategies.

COMPARATIVE DOCKING ANALYSIS REVEALS DIVERGENT SGLT2 SELECTIVITY, FAVORING DAPAGLIFLOZIN I Dewa Agung Panji Dwipayana; Ni Luh Putu Kayika Febryanti; Made Pharmawati
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 15, No 4 (2026): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v15i4.53412

Background: Sodium-glucose cotransporter 2 (SGLT2) inhibitors are widely used in type 2 diabetes therapy, but selectivity over SGLT1 is critical to minimize gastrointestinal side effects. Objectives: To evaluate and compare the selectivity of SGLT inhibitors, especially Dapagliflozin and Empagliflozin, toward SGLT1 and SGLT2 through molecular docking to inform future inhibitor design. Methods: This study used molecular docking to compare the binding profiles of five SGLT inhibitors, Dapagliflozin, Empagliflozin, Canagliflozin, Sotagliflozin, and LX2761, against SGLT1 and SGLT2 transporters Results: Dapagliflozin was the only compound to show statistically significant selectivity for SGLT2 over SGLT1 (p = 0.036), while Empagliflozin and others lacked significant differences in isoform affinity. Although Empagliflozin exhibited slightly stronger nominal binding energy to SGLT2 than Dapagliflozin, ligand efficiency (LE) analysis indicated that this was primarily due to its larger molecular size, affirming Dapagliflozin’s more efficient binding. Interaction analysis further revealed that Dapagliflozin formed a clean, stabilizing pose within the SGLT2 pocket, free of repulsive interactions, unlike Empagliflozin, which displayed an unfavorable donor-donor contact in the same site. Conclusion: These findings support the structural basis for Dapagliflozin’s higher selectivity and reinforce its profile as a SGLT2-specific agent.

The Antagonistic Potential of Kimchi-Associated LAB Against Histamine-Producing Pathogens in Seafood Putu Satrio Jhanardana Dewantara; Ni Putu Bintang Diah Lastini; Made Okta Hapsari Putri Diarsa; Pande Gede Putra Satwika; Dwi Ariani Yuliastuti; I Dewa Agung Panji Dwipayana; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p3

Lactic acid bacteria (LAB) play a crucial role in food fermentation, for example in kimchi, a traditional Korean dish. These bacteria don’t only function in food preservation and probiotics, but also exhibit antimicrobial activity through the production of compounds like bacteriocins. This study introduces a novel approach in using LAB from kimchi for inhibiting the growth of Morganella morganii, a major histamine-producing pathogen in fish products. Histamine accumulation poses severe food safety risks, necessitating an innovative natural solutions. LAB isolates from both solid and liquid fractions of kimchi were screened on selective media and characterized biochemically for traits such as fermentation type and acid production. Antimicrobial efficacy, assessed via the Kirby-Bauer disk diffusion method, revealed inhibition zones averaging 6.98 ± 0.04 mm for liquid and 6.40 ± 1.34 mm for solid fractions. Despite their lower efficacy compared to chloramphenicol (30.80 ± 1.92 mm), the eco-friendly nature of LAB highlights their unique potential as a sustainable alternative for histamine control. This study is among the first to explore kimchi-derived LAB as antagonists against M. morganii, providing a novel perspective for developing green technologies in food safety and quality management.

A Review of Molecular Approaches for Sex Determination in Ornamental Fish I Dewa Agung Panji Dwipayana; Ni Luh Putu Kayika Febryanti; I Gusti Ayu Nadia Prasta Unique
Metamorfosa: Journal of Biological Sciences Vol. 13 No. 1 (2026)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2026.v13.i01.p8

The ornamental fish industry is a globally significant sector of aquaculture in which accurate sex identification is essential for optimizing breeding efficiency, economic value, and stock management. However, traditional phenotypic sexing methods are often unreliable due to late sexual dimorphism, environmental influences, and extensive strain divergence driven by domestication and selective breeding. This review synthesizes current knowledge on the biological basis of sex determination in ornamental fishes and evaluates molecular approaches used for sex identification across diverse genetic and environmental contexts. Teleost fishes exhibit exceptional diversity in sex determination systems, including XX/XY, ZZ/ZW, polygenic, and epigenetic system. Such diversity is often accompanied by selective breeding which may shuffle sex-determining regions. Consequently, no single molecular marker is universally applicable across ornamental taxa. PCR-based methods, such as SCAR, RAPD, AFLP, and InDel markers, remain the most accessible tools and are effective within validated populations, while next-generation sequencing enables genome-wide discovery of sex-linked SNPs and structural variants to provide high-resolution and reproducible diagnostics. Transcriptomic and epigenetic markers offer mechanistic insight into sex differentiation particularly in plastic or temperature-sensitive systems, although they are limited as standalone diagnostic tools. Emerging portable and point-of-care platforms show promise for on-site sex identification but require further validation in fish. This review emphasizes that robust molecular sexing in ornamental fish requires careful marker selection, multi-strain validation, and integration of genetic, epigenetic, and environmental data. Expanding genomic resources and standardized validation frameworks will be critical for translating molecular sexing into reliable, industry-wide applications.

The Antagonistic Potential of Kimchi-Associated LAB Against Histamine-Producing Pathogens in Seafood Putu Satrio Jhanardana Dewantara; Ni Putu Bintang Diah Lastini; Made Okta Hapsari Putri Diarsa; Pande Gede Putra Satwika; Dwi Ariani Yuliastuti; I Dewa Agung Panji Dwipayana; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p3

Lactic acid bacteria (LAB) play a crucial role in food fermentation, for example in kimchi, a traditional Korean dish. These bacteria don’t only function in food preservation and probiotics, but also exhibit antimicrobial activity through the production of compounds like bacteriocins. This study introduces a novel approach in using LAB from kimchi for inhibiting the growth of Morganella morganii, a major histamine-producing pathogen in fish products. Histamine accumulation poses severe food safety risks, necessitating an innovative natural solutions. LAB isolates from both solid and liquid fractions of kimchi were screened on selective media and characterized biochemically for traits such as fermentation type and acid production. Antimicrobial efficacy, assessed via the Kirby-Bauer disk diffusion method, revealed inhibition zones averaging 6.98 ± 0.04 mm for liquid and 6.40 ± 1.34 mm for solid fractions. Despite their lower efficacy compared to chloramphenicol (30.80 ± 1.92 mm), the eco-friendly nature of LAB highlights their unique potential as a sustainable alternative for histamine control. This study is among the first to explore kimchi-derived LAB as antagonists against M. morganii, providing a novel perspective for developing green technologies in food safety and quality management.

A Review of Molecular Approaches for Sex Determination in Ornamental Fish I Dewa Agung Panji Dwipayana; Ni Luh Putu Kayika Febryanti; I Gusti Ayu Nadia Prasta Unique
Metamorfosa: Journal of Biological Sciences Vol. 13 No. 1 (2026)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2026.v13.i01.p8

The ornamental fish industry is a globally significant sector of aquaculture in which accurate sex identification is essential for optimizing breeding efficiency, economic value, and stock management. However, traditional phenotypic sexing methods are often unreliable due to late sexual dimorphism, environmental influences, and extensive strain divergence driven by domestication and selective breeding. This review synthesizes current knowledge on the biological basis of sex determination in ornamental fishes and evaluates molecular approaches used for sex identification across diverse genetic and environmental contexts. Teleost fishes exhibit exceptional diversity in sex determination systems, including XX/XY, ZZ/ZW, polygenic, and epigenetic system. Such diversity is often accompanied by selective breeding which may shuffle sex-determining regions. Consequently, no single molecular marker is universally applicable across ornamental taxa. PCR-based methods, such as SCAR, RAPD, AFLP, and InDel markers, remain the most accessible tools and are effective within validated populations, while next-generation sequencing enables genome-wide discovery of sex-linked SNPs and structural variants to provide high-resolution and reproducible diagnostics. Transcriptomic and epigenetic markers offer mechanistic insight into sex differentiation particularly in plastic or temperature-sensitive systems, although they are limited as standalone diagnostic tools. Emerging portable and point-of-care platforms show promise for on-site sex identification but require further validation in fish. This review emphasizes that robust molecular sexing in ornamental fish requires careful marker selection, multi-strain validation, and integration of genetic, epigenetic, and environmental data. Expanding genomic resources and standardized validation frameworks will be critical for translating molecular sexing into reliable, industry-wide applications.

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