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All Journal MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM ODONTO Dental Journal Odonto dental journal
Rudhanton, Rudhanton
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Dentistry Health Professions Medicine & Pharmacology

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Tooth extrinsic stain remover by Averrhoa bilimbi L extract solution Listari, Khusnul Munika; Rudhanton, Rudhanton; Nugraeni, Yuli; Salsabila, Syafira
Odonto : Dental Journal Vol 12, No 3 (2025): December 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.3.292-299

Abstract

Background : Dental stains are pigmented deposits that can be a predisposing factor for periodontal disease and caused aesthetic problems. Chromogens from foods and beverages such as coffee can adhere to the tooth surface trough pellicle and forming deposits called tooth extrinsic stains. Averrhoa bilimbi L contains oxalic acid which has potential ability to remove it trough oxidation mechanisms. The aim of this study was to investigate the effectiveness of Averrhoa bilimbi L extract solution on extrinsic tooth stain removal.Method : Tooth extrinsic stain models obtained by soaking the teeth in robusta coffee solution. Twenty-four post extraction premolar teeth with extrinsic stains were divided into 4 groups consisting of control group (immersion in artificial saliva), T1 group(immersion in Averrhoa bilimbi L extract solution 50%), T2 group (immersion in Averrhoa bilimbi L extract solution 75%) and T3 group (immersion in Averrhoa bilimbi L extract solution 100%) for 28 hours. Adobe Photoshop CC 2018 used to evaluate the difference color intensity (ΔE) in CieLAB system before and after the treatment.Result : Anova test showed there’s a significant difference within groups (p<0.05). Post Hoc Posttest showed there are  significant difference of difference color intensity (ΔE) between each group(p<0.05), with the highest average of it on T3 group then followed by T2 group , T1 group and control. Pearson correlation analysis showed there is a positive correlation between concentration and color intensity.Conclusion : Averrhoa bilimbi L. extract solution proven effective as extrinsic stain removal in vitro.
Tooth extrinsic stain remover by Averrhoa bilimbi L extract solution Listari, Khusnul Munika; Rudhanton, Rudhanton; Nugraeni, Yuli; Salsabila, Syafira
Odonto : Dental Journal Vol 12, No 3 (2025): December 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.3.292-299

Abstract

Background : Dental stains are pigmented deposits that can be a predisposing factor for periodontal disease and caused aesthetic problems. Chromogens from foods and beverages such as coffee can adhere to the tooth surface trough pellicle and forming deposits called tooth extrinsic stains. Averrhoa bilimbi L contains oxalic acid which has potential ability to remove it trough oxidation mechanisms. The aim of this study was to investigate the effectiveness of Averrhoa bilimbi L extract solution on extrinsic tooth stain removal.Method : Tooth extrinsic stain models obtained by soaking the teeth in robusta coffee solution. Twenty-four post extraction premolar teeth with extrinsic stains were divided into 4 groups consisting of control group (immersion in artificial saliva), T1 group(immersion in Averrhoa bilimbi L extract solution 50%), T2 group (immersion in Averrhoa bilimbi L extract solution 75%) and T3 group (immersion in Averrhoa bilimbi L extract solution 100%) for 28 hours. Adobe Photoshop CC 2018 used to evaluate the difference color intensity (ΔE) in CieLAB system before and after the treatment.Result : Anova test showed there’s a significant difference within groups (p<0.05). Post Hoc Posttest showed there are  significant difference of difference color intensity (ΔE) between each group(p<0.05), with the highest average of it on T3 group then followed by T2 group , T1 group and control. Pearson correlation analysis showed there is a positive correlation between concentration and color intensity.Conclusion : Averrhoa bilimbi L. extract solution proven effective as extrinsic stain removal in vitro.
Pengaruh konsentrasi gel high molecular weight hyaluronic acid terhadap jumlah fibroblas gingiva tikus Wistar Fauzia, Malianawati; Jamal, Revana Anjelina Cantika; Lestari, Khusnul Munika; Fuadiyah, Diena; Rudhanton, Rudhanton
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 12, No 1 (2026)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.114941

Abstract

Penyembuhan luka gingiva merupakan proses biologis kompleks yang melibatkan berbagai respons seluler, terutama aktivitas fibroblas sebagai komponen utama pembentukan jaringan ikat. High molecular weight Hyaluronic Acid (HMW-HA) dikenal memiliki peran penting dalam mendukung proses penyembuhan luka melalui regulasi migrasi dan proliferasi sel serta pembentukan matriks ekstraseluler. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian gel HMW-HA dengan berbagai konsentrasi terhadap jumlah fibroblas pada penyembuhan luka gingiva tikus Wistar. Penelitian ini merupakan penelitian eksperimental laboratoris dengan rancangan post-test only control group design menggunakan 28 ekor tikus Wistar jantan yang dibagi ke dalam tujuh kelompok, terdiri atas kelompok kontrol negatif, kontrol positif (Gengigel® 0,2%), serta lima kelompok perlakuan gel HMW-HA  dengan konsentrasi 0,2%, 0,4%, 0,6%, 0,8%, dan 1%. Luka standar dibuat pada gingiva, diikuti pemberian gel secara topikal selama tujuh hari. Jaringan gingiva kemudian diperiksa secara histopatologis menggunakan pewarnaan Hematoxylin–Eosin untuk menghitung jumlah fibroblas. Hasil penelitian menunjukkan adanya variasi jumlah fibroblas antar kelompok perlakuan, dengan rerata tertinggi pada kelompok gel HMW-HA  konsentrasi 1% dan terendah pada kelompok kontrol negatif. Analisis statistik menggunakan One-Way ANOVA menunjukkan bahwa perbedaan jumlah fibroblas antar kelompok tidak signifikan secara statistik (p > 0,05), namun secara deskriptif terlihat kecenderungan peningkatan jumlah fibroblas seiring dengan meningkatnya konsentrasi gel HMW-HA. Dari hasil penelitian disimpulkan bahwa gel HMW-HA berpotensi mendukung proses penyembuhan luka gingiva melalui peningkatan respons seluler fibroblas, meskipun diperlukan penelitian lanjutan untuk mengonfirmasi signifikansi klinis temuan ini.
Co-Authors Fauzia, Malianawati Fuadiyah, Diena Jamal, Revana Anjelina Cantika Lestari, Khusnul Munika listari, khusnul munika Salsabila, Syafira Yuli Nugraeni, Yuli