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All Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Damanik, Asman Ramadhan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions

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CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Damanik, Asman Ramadhan; Karja, Ni Wayan Kurniani; Setiadi , Mohamad Agus; Kaiin, Ekayanti Mulyawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Damanik, Asman Ramadhan; Karja, Ni Wayan Kurniani; Setiadi , Mohamad Agus; Kaiin, Ekayanti Mulyawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10407

Abstract

This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation.
Co-Authors Kaiin, Ekayanti Mulyawati Ni Wayan Kurniani Karja Setiadi , Mohamad Agus