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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Sain Veteriner Buletin Peternakan Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Kedokteran Hewan Annales Bogorienses
Kaiin, Ekayanti Mulyawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Use of Collagenase Enzyme as a Substitute for Trypsin on In Vitro Growth of Liver, Kidney, and Fibroblasts of Chicken Embryo Cells Ananda, Ananda; Kaiin, Ekayanti Mulyawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.366 KB) | DOI: 10.33772/jitro.v9i2.23636

Abstract

This study aims to determine the effect of using the collagenase enzyme on the viability of chicken embryos' liver, kidney, and fibroblast cells in vitro. Liver and kidney organs were collected from embryonic chicken eggs at 15 and 19 days. Meanwhile, fibroblast cells were obtained using muscle tissue of 11-day-old chicken embryos. Each organ and tissue was chopped and incubated using 0.1% trypsin for 10 minutes and 0.1% collagenase for 30 minutes. Concentration, viability, and proliferation rate were calculated after incubation and analyzed using a t-test. The results showed that there was a significant increase in the tissue incubated with collagenase on the viability and proliferation rate of liver cells, kidney cells, and chicken embryo fibroblast cells (t<0.05), and not significant in the acquisition of cell concentrations (t>0, 05). Cell isolation and purification techniques need to be carried out to study the quality of somatic cell culture of chicken embryos more specifically.Keywords: collagenase, trypsin, chicken embryos, viability, cell culture.
Performance and Physicochemical Quality of Quail Eggs Given Bandotan (Ageratum conyzoides L.) Leaf Extract Hariono, Hariono; Kaiin, Ekayanti Mulyawati; Suryati, Tuti; Ulupi, Niken; Afnan, Rudi
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.780

Abstract

The purpose of this study was to determine the influence of bandotan (Ageratum conyzoides L.) leaf extract on the production performance and physicochemical quality of quail eggs raised in tropical settings with ambient temperatures that exceed the comfort zone. A total of 200 female quails aged 6 weeks were reared in colony cages for 30 days. They were separated into four treatments with five replications, each with ten quails. Bandotan leaf extract was administered in dosages of 0%, 0.15%, 0.30%, and 0.45% per quail daily. The parameters measured included feed intake, feed conversion, egg production, egg mass, mortality, egg physical quality, and cholesterol and malondialdehyde (MDA) levels in egg yolks. Adding bandotan leaf extract to drinking water significantly improved quail production performance (p < 0.05). A dose of 0.30% resulted in the highest feed consumption, output, and egg mass, as well as significantly lower mortality rates, indicating its effectiveness in boosting the productivity and health of laying quails. Although it did not have a significant influence on the physical quality of eggs, administration of the extract at that dose significantly reduced MDA levels, egg cholesterol levels, and boosted antioxidant activity (p < 0.05). This demonstrates that bandotan leaf extract at a dose of 0.30% is beneficial in improving the production performance and chemical quality of heat-stressed quail eggs while preserving their physical quality. Keywords: bandotan, eggs, malondialdehyde, productive performance, quail
Aktivitas Farmakologis Ekstrak Daun Bandotan (Ageratum conyzoides L.) dalam Bidang Kesehatan Hewan: Pharmacological Activity of Bandotan Leaf Extract (Ageratum conyzoides L.) in Animal Health Hariono, Hariono; Kaiin, Ekayanti Mulyawati; Suryati, Tuti; Ulupi, Niken; Afnan, Rudi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 27 No 3 (2025): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.27.3.179-188.2025

Abstract

Kesehatan hewan memiliki peranan yang sangat penting dalam mendukung produktivitas ternak, kesejahteraan hewan, serta kesehatan masyarakat veteriner. Seiring dengan meningkatnya kekhawatiran terhadap dampak negatif penggunaan obat-obatan kimia, seperti antibiotik dan antioksidan sintetis, khususnya yang berkaitan dengan risiko resistensi antimikroba, toksisitas, serta residu, muncul kebutuhan akan alternatif alami yang lebih aman dan berkelanjutan. Salah satu tanaman yang berpotensi memenuhi kebutuhan tersebut adalah bandotan (Ageratum conyzoides Linn.). Tanaman ini mengandung berbagai senyawa fitokimia seperti flavonoid, fenol, alkaloid, dan tanin yang memiliki aktivitas farmakologis sebagai antioksidan, antimikroba, antiinflamasi, serta membantu menurunkan kadar kolesterol. Penelitian ini merupakan studi literatur dengan analisis deskriptif terhadap data sekunder dari jurnal dan artikel terakreditasi nasional dan internasional. Berdasarkan kajian pustaka dari berbagai sumber ilmiah, Ekstrak daun bandotan efektif menghambat peroksidasi lipid, menurunkan kolesterol dan trigliserida, serta meningkatkan bobot badan pada tikus. Pada ayam broiler, ekstrak ini menurunkan kolesterol, meningkatkan pertumbuhan, efisiensi pakan, kualitas daging, dan berfungsi sebagai agen antikoksidia alami.
PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Satrio, Faisal Amri; Karja, Ni Wayan Kurniani; Setiadi, Mohamad Agus; Kaiin, Ekayanti Mulyawati; Kurnia, Asep; Purwantara, Bambang
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23487

Abstract

This study aimed to determine the effect of age on bulls productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, 10 yo with high semen rejection (10 HR), and 10 yo with low semen rejection (10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of 10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in 10 yo groups. The 10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of 10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in 10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.
THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES Nurkarimah, Dona Astari; Kaiin, Ekayanti Mulyawati; Karja, Ni Wayan Kurniani; Setiadi, Mohamad Agus
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.17688

Abstract

The purpose of this study was to evaluate the effectiveness of cysteamine supplementation on the maturation medium and/or in vitro fertilization medium with regards to improving the normal fertilization rate of sheep oocytes, which are characterized by the formation of two pronuclei. Grade A and B oocytes were matured in medium-199 with 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), 10 IU/mL human chorionic gonadotrophin (hCG), and 50 g/mL gentamicin added for 24 hours in a 5% CO2 incubator at 39 C. The treatment group was divided into the following groups: a control group with no cysteamine (P1), a group with 100 M cysteamine in the maturation medium (P2), a group with 100 M cysteamine in the fertilization medium (P3), and a group with 100 M cysteamine both in the maturation and fertilization medium (P4). The fertilization was carried out by incubating sperm-oocytes for 12 hours and then staining them with 1% aceto-orcein to observe the formation of a pronucleus. Normal fertilization rates obtained by each treatment group were 56.5% (P1), 57.1% (P2), 57.8% (P3), and 59.9% (P4) with no significant difference (P0.05) between groups. It was concluded that 100 M cysteamine supplementation in both the maturation medium and fertilization medium was not able to increase the normal fertilization rate of sheep oocytes.
INFLUENCES OF INCUBATION TIME AND SUCROSE CONCENTRATION ON MICE (Mus musculus L.) OOCYTE VIABILITY FOR ENUCLEATING PROCEDURE Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Madihah, Madihah; Nafisah, Ghina
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.10896

Abstract

This study aimed to find out the optimum incubation time to complete mouse oocyte maturation at Metaphase II (MII) stage and determine the optimum sucrose concentration enabling to induce nuclear swelling for visualization that is important for enucleating process at the initial procedure of somatic cell nuclear transfer (SCNT). In this current study, mice were used as animal model. Completely randomized design was arranged, consists of 2 trials with 4 treatments and 7 replications. In the first trial, the oocytes were cultured at 0-2, 4-6, 8-10, and 12-14 h in 5% CO2 incubator at 37 C. Second, the MII oocytes obtained from previous trial were cultured in M199 medium containing different concentrations of sucrose (0, 1.5, 3, and 6%). The parameters measured were the oocyte viability at various stages, i.e germinal vesicle (GV), metaphase I (MI), anaphase/telophase I (A/T I), and metaphase II (MII), and the viability of swollen nuclear oocytes using Hoechst/PI staining. The results showed that the optimum incubation time required by oocytes to reach MII stage was 12-14 h with a percentage of 57.1412.67%, while the optimum sucrose concentration for nuclear swelling was found at 3% with a percentage of 1000.00%. Our findings provided preliminary results related to the maturation process of the mouse oocyte nucleus, which is meaningful for the initial procedure of SCNT.
EVALUATION OF RAT LEYDIG CELL CULTURE COLLECTED WITH NYCODENZ GRADIENT IN PRODUCING TESTOSTERONE IN VITRO Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.3984

Abstract

The aim of this study was to evaluate the ability of rats Leydig cells collected with Nycodenz gradient in producing testosterone in vitro. Leydig cells were collected using 5 column of Nycodenz gradient (4, 8, 10, 12, and 15%) and cells were evaluated regarding its concentration, viability, and purity of Leydig cells. Media used to cultured Leydig cells were Dulbeccos Modified Eagles Medium (DMEM)+10% newborn calf serum (NBCS); DMEM+10% NBCS+2,5 IU/mL human chorionic gonadotrophin (hCG); DMEM+10% NBCS+ 5 g/mL insulin, 10 g/mL transferrin, and 5 g/mL Se (ITS); DMEM+10% NBCS+hCG+ITS at 5% CO2 incubator with temperature of 37.5 C for 3 days. Culture medium was collected every day for testosterone analysis with enzyme-linked immunosorbent assay (ELISA). By adding ITS to the medium, Leydig cells concentration was significantly increased (8.92x106 cells/mL) compared to medium with serum (7.74x106 cells/mL) or hCG (7.68x106 cells/mL) (P0.05). ITS and hCG in medium significantly increased Leydig cells concentration (10.40x106 cells/mL) at day 3 of culture (P0.05). The result of parallelism test showed that the assay obtained good validity to measure testosterone concentration in culture medium. Testosterone in medium was detected at 1.80-2.60 ng/mL at day 1 of culture. In conclusion, Leydig cells collected with Nycodenz gradient had no effect to testosterone secretion from Leydig cells in vitro.
Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.) Gustina, Sri; Hasbi, Hasbi; Sonjaya, Herry; Baco, Sudirman; Qhatimah, Husnul; Saputra, Wandi; Mutmainna, Mutmainna; Kaiin, Ekayanti Mulyawati; Maulana, Tulus
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86154

Abstract

            The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P<0.01). Motility and acrosome integrity of frozen semen were not significantly difference (P>0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity.
Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP Method Agung, Paskah Partogi; Perwitasari, Dyah; Farajallah, Achmad; Said, Syahruddin; Kaiin, Ekayanti Mulyawati; Saputra, Ferdy; Hermansyah, Alfandy; Kuswati, Kuswati; Susilorini, Tri Eko; Zein, Moch. Syamsul Arifin
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.87484

Abstract

The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
Development of Mice Embryo (Mus musculus L.) after Closed Pulled Straw Vitrification in CZB Medium Gunawan, Muhammad; Kaiin, Ekayanti Mulyawati; Rusherdiannita, Raden Cindy; Alipin, Kartiawati
Annales Bogorienses Vol. 23 No. 2 (2019): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of our present experiment was to investigate the effects of closed pulled straw (CPS) vitrification on the viability and development of mouse embryo. The experiment was arranged according to completely randomized design (CRD) consisting of 4 treatments, namely not vitrification (NV) is control, CPS 1 (mCZB Hepes + 20% Bovine Serum Albumin (BSA) +0.5 M sucrose + 10% EG + 10% DMSO), CPS 2 (mCZB Hepes + 20% BSA + 0.5 M sucrose + 15% EG + 15% DMSO), and CPS 3 (mCZB Hepes + 20% BSA +0.5 M sucrose + 20% EG + 20% DMSO) with 6 replications. The viability of embryos (%) was determined after 24 - 72 h of the culture period, while we also observed the percentage of embryos reaching the blastocyst stage (early blastocyst, expanded, hatching, and hatched). As a result, the treatments did not give a significant difference in the viability of embryos (P<0,05) but showed significant effects on embryo development (P<0,05). Furthermore, this present work conclusively found that CPS vitrification in CZB medium with cryoprotectants ethylene glycol (EG) and dimethyl sulfoxide (DMSO) noticeably influenced the development of mice embryo to reach the blastocyst stage, but showed no remarkable difference in the viability of embryo after culture for 24 – 72 h.
Co-Authors ACHMAD FARAJALLAH Ananda, Ananda Bambang Purwantara Dyah Perwitasari Faisal Amri Satrio Ferdy Saputra Hariono Hariono Hasbi Hermansyah, Alfandy Herry Sonjaya ITA DJUWITA Kartiawati Alipin Kurnia, Asep Kusdiantoro Mohamad Kuswati Kuswati M Agus Setiadi Madihah Madihah, Madihah Maulana, Tulus Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Muhammad Gunawan Mutmainna, Mutmainna Nafisah, Ghina Ni Wayan Kurniani Karja Niken Ulupi Nurkarimah, Dona Astari Paskah Partogi Agung Qhatimah, Husnul Rudi Afnan Rusherdiannita, Raden Cindy Saputra, Wandi Sri Gustina Sudirman Baco, Sudirman Syahruddin Said Tri Eko Susilorini Tuti Suryati TUTY LASWARDI YUSUF Wahono Esthi Prasetyaningtyas