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All Journal Jurnal Ilmu Pertanian Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis)
Kaiin, Ekayanti Mulyawati
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Agriculture, Biological Sciences & Forestry Veterinary

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Use of Collagenase Enzyme as a Substitute for Trypsin on In Vitro Growth of Liver, Kidney, and Fibroblasts of Chicken Embryo Cells Ananda, Ananda; Kaiin, Ekayanti Mulyawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.366 KB) | DOI: 10.33772/jitro.v9i2.23636

Abstract

This study aims to determine the effect of using the collagenase enzyme on the viability of chicken embryos' liver, kidney, and fibroblast cells in vitro. Liver and kidney organs were collected from embryonic chicken eggs at 15 and 19 days. Meanwhile, fibroblast cells were obtained using muscle tissue of 11-day-old chicken embryos. Each organ and tissue was chopped and incubated using 0.1% trypsin for 10 minutes and 0.1% collagenase for 30 minutes. Concentration, viability, and proliferation rate were calculated after incubation and analyzed using a t-test. The results showed that there was a significant increase in the tissue incubated with collagenase on the viability and proliferation rate of liver cells, kidney cells, and chicken embryo fibroblast cells (t<0.05), and not significant in the acquisition of cell concentrations (t>0, 05). Cell isolation and purification techniques need to be carried out to study the quality of somatic cell culture of chicken embryos more specifically.Keywords: collagenase, trypsin, chicken embryos, viability, cell culture.
Performance and Physicochemical Quality of Quail Eggs Given Bandotan (Ageratum conyzoides L.) Leaf Extract Hariono, Hariono; Kaiin, Ekayanti Mulyawati; Suryati, Tuti; Ulupi, Niken; Afnan, Rudi
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.780

Abstract

The purpose of this study was to determine the influence of bandotan (Ageratum conyzoides L.) leaf extract on the production performance and physicochemical quality of quail eggs raised in tropical settings with ambient temperatures that exceed the comfort zone. A total of 200 female quails aged 6 weeks were reared in colony cages for 30 days. They were separated into four treatments with five replications, each with ten quails. Bandotan leaf extract was administered in dosages of 0%, 0.15%, 0.30%, and 0.45% per quail daily. The parameters measured included feed intake, feed conversion, egg production, egg mass, mortality, egg physical quality, and cholesterol and malondialdehyde (MDA) levels in egg yolks. Adding bandotan leaf extract to drinking water significantly improved quail production performance (p < 0.05). A dose of 0.30% resulted in the highest feed consumption, output, and egg mass, as well as significantly lower mortality rates, indicating its effectiveness in boosting the productivity and health of laying quails. Although it did not have a significant influence on the physical quality of eggs, administration of the extract at that dose significantly reduced MDA levels, egg cholesterol levels, and boosted antioxidant activity (p < 0.05). This demonstrates that bandotan leaf extract at a dose of 0.30% is beneficial in improving the production performance and chemical quality of heat-stressed quail eggs while preserving their physical quality. Keywords: bandotan, eggs, malondialdehyde, productive performance, quail
Co-Authors Ananda, Ananda Hariono Hariono Niken Ulupi Rudi Afnan Tuti Suryati