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Ekstraksi Protein Bungkil Sawit serta Karakterisasinya dengan FTIR dan Uji Kjeldahl Yogananda, Amrina Amalia; Alfian, Muhammad; Hidayati, Listiana; Yulianti, Selvia; Cahyani Irawan, Hisba; Restu Dwi Saputro, Rafly; Cita Cahyani, Anggun
Jurnal Riset Farmasi Volume 5, No. 2, Desember 2025, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrf.v5i2.8207

Abstract

Abstract. Palm kernel cake is a byproduct of the palm oil industry that remains underutilized despite its high content of non-starch polysaccharides and carbohydrate-bound proteins with potential as plant-based biomaterials. This study aimed to extract protein from palm kernel cake and identify its functional groups and protein content using Fourier Transform Infrared (FTIR) spectroscopy and the Kjeldahl method. Protein extraction was carried out using an alkali–acid process involving solubilization with NaOH followed by isoelectric precipitation with HCl. The dried protein extract was analyzed using FTIR to detect characteristic protein absorption bands, while protein content was quantified using the Kjeldahl method. The extraction produced a yield of 9.6%. FTIR spectra showed the presence of Amide I (1650 cm⁻¹), Amide II (1540 cm⁻¹), and Amide III (1230 cm⁻¹) bands, confirming the protein structure within the extract. The Kjeldahl analysis revealed a protein content of 49.27%, which is higher than previously reported values and exceeds the minimum protein requirement of 13% set by the Indonesian National Standard (SNI). These findings demonstrate that palm kernel cake is a promising raw material for the development of plant-derived protein suitable for pharmaceutical and biomaterial applications. Abstrak. Bungkil sawit merupakan produk samping industri minyak sawit yang masih belum dimanfaatkan secara optimal, meskipun mengandung polisakarida bukan pati dan protein terikat yang berpotensi sebagai sumber biomaterial nabati. Penelitian ini bertujuan untuk mengekstraksi protein dari bungkil sawit serta mengidentifikasi struktur fungsional dan kadarnya menggunakan spektroskopi Fourier Transform Infrared (FTIR) dan metode Kjeldahl. Proses ekstraksi dilakukan menggunakan metode alkali–asid dengan pelarutan NaOH dan presipitasi isoelektrik menggunakan HCl. Sampel protein kering dianalisis menggunakan FTIR untuk mendeteksi pita-pita karakteristik protein, sedangkan kadar protein ditentukan secara kuantitatif dengan metode Kjeldahl. Hasil penelitian menunjukkan rendemen protein sebesar 9,6%. Spektrum FTIR memperlihatkan pita Amida I pada 1650 cm⁻¹, Amida II pada 1540 cm⁻¹, dan Amida III pada 1230 cm⁻¹ yang mengonfirmasi keberadaan struktur protein. Uji Kjeldahl menunjukkan kadar protein sebesar 49,27%, lebih tinggi daripada nilai yang dilaporkan pada penelitian sebelumnya dan telah memenuhi standar SNI minimal 13%. Temuan ini menunjukkan bahwa bungkil sawit berpotensi sebagai sumber protein nabati untuk pengembangan aplikasi farmasi dan biomaterial.
Identifikasi dan Analisis Galaktomanan dari Bungkil Sawit sebagai Bahan Baku Biomaterial dengan Metode FTIR dan NMR Yogananda, Amrina Amalia; Alfian, Muhammad; Faizah, Nurul; Cahyani , Anggun Cita; Yulianti, Selvia; Irawan , Hisba Cahyani; Saputro, Rafly Restu Dwi
BORNEO JOURNAL OF PHARMASCIENTECH Vol 10 No 1 (2026): Borneo Journal of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59053/bjp.v10i1.756

Abstract

Palm kernel meal contains polysaccharide compounds, including galactomannan, which is made up of galactose and mannose units. This study aims to extract and identify the structure of galactomannan from palm kernel meal using FTIR and NMR spectroscopy. Extraction was carried out using a hot water extraction method with a material-to-solvent ratio of 1:5 at 80°C for 60 minutes, followed by precipitation with 96% ethanol, subsequent freeze-drying, and followed by characterization through FTIR and NMR to confirm the presence of characteristic functional groups associated with galactomannan. The extraction yielded 0,77% then the FTIR spectrum revealed strong bands at 1065.67, 1032.65, and 1009.16 cm⁻¹, indicating the presence of C–O and C–O–C stretching related to glycosidic bonds—key features of the galactomannan polysaccharide structure. The 1H-NMR spectrum exhibited a distinct signal for the anomeric proton in the range of approximately 5.0–5.4 ppm, with the protons from the C2–C6 positions appearing between 3.2 and 4.1 ppm. Additionally, the 13C-NMR spectrum displayed a peak for the anomeric carbon around 102–105 ppm, along with other ring carbon peaks in the range of 60–85 ppm, which align with the structure of galactomannan. These findings confirm that palm kernel meal holds promise as a source of galactomannan for developing polysaccharide-based biomaterials