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Mailayfaiza, Septiandita
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Biochemistry, Genetics & Molecular Biology

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Introduction to Gel Electrophoresis Lab - Virtual Simulation in Biotechnology Course Ria, Ria; Solihin, Solihin; Mailayfaiza, Septiandita; Syafkitamsana, Najwa; Harahap, Lia Junita
Bioedunis Journal Vol 4, No 2 (2025)
Publisher : UIN Syekh Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/bioedunis.v4i2.18844

Abstract

Gel electrophoresis is a fundamental technique in molecular biology used to separate DNA fragments by size. However, limitations in laboratory facilities often hinder its implementation. This study aims to analyze students’ understanding of the operating principles of gel electrophoresis, the functions of its main components, and their ability to interpret DNA separation results through a virtual practicum on the LabXchange platform. The study employed a descriptive qualitative approach with 63 fifth-semester students from the Biology Education Program. Data were collected through observations during the simulation, analysis of DNA band patterns generated by the virtual experiment, and students’ responses in the worksheet. The findings indicate that the virtual simulation effectively visualizes the DNA migration process, enabling students to understand the roles of agarose gel, buffer, and electrical current in fragment separation. Students identified the relationship between fragment size and migration distance and evaluated the expected results based on electrophoresis theory. Thus, the virtual laboratory practicum proved to be an effective alternative learning method for enhancing conceptual understanding and analytical skills in gel electrophoresis.
Introduction to Bioethanol Lab on Cassava for Biology Education Students at UIN Jakarta Syafkitamsana, Najwa; Solihin, Solihin; Ria, Ria; Mailayfaiza, Septiandita
Bioedunis Journal Vol 4, No 2 (2025)
Publisher : UIN Syekh Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/bioedunis.v4i2.18846

Abstract

This study discusses conventional bioethanol production through fermentation as a response to the need for environmentally friendly alternative energy sources. The objectives of this practicum are to understand the basic principles of fermentation, identify the factors that influence it, carry out a simple fermentation process using natural materials (sugar/corn/cassava) and Saccharomyces cerevisiae yeast, and analyze the results. The methods used include preparing a sugar solution, inoculating yeast, anaerobic incubation at 30–35 °C for 2–5 days, observing CO₂ formation, filtration, and simple purification through distillation. The study employed an experimental design, with 63 biology education students participating. The results of the practicum indicate that students understand the success rate of bioethanol fermentation, as evidenced by the formation of CO₂ bubbles and the feasibility of ethanol separation by distillation. Students can understand the factors that influence conventional bioethanol production.
Introduction to Virtual Lab Polymerase Chain Reaction (PCR) Practicals in Biology Education at UIN Jakarta Solihin, Solihin; Mailayfaiza, Septiandita; Ria, Ria; Syafkitamsana, Najwa; Harahap, Lia Junita; Giriyanti, Putri
Bioedunis Journal Vol 4, No 2 (2025)
Publisher : UIN Syekh Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/bioedunis.v4i2.18842

Abstract

Polymerase Chain Reaction (PCR) is a fundamental biotechnology technique used to amplify specific DNA fragments in large quantities through in vitro replication. This virtual laboratory aims to (1) understand the basic principles and working stages of PCR, (2) identify the main functions of PCR components, and (3) apply PCR simulations to amplify target genes using the LabXchange platform. The research was conducted virtually, using an interactive experimental approach, by 60 Biology Education students working in one group. Data were collected from student worksheets and simulation outputs. The results showed that students were able to identify and explain each stage of the PCR process, including denaturation, annealing, and extension, as well as analyze the roles of the DNA template, primers, dNTPs, buffer, and Taq polymeraseenzyme. The simulation successfully demonstrated efficient amplification of the target gene. These findings indicate that virtual laboratory learning effectively enhances students’ conceptual understanding and analytical skills in biotechnology.
Co-Authors Giriyanti, Putri Harahap, Lia Junita Ria, Ria Solihin Solihin Syafkitamsana, Najwa