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All Journal Bioedunis Journal
Giriyanti, Putri
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Biochemistry, Genetics & Molecular Biology

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Introduction to Virtual Lab Polymerase Chain Reaction (PCR) Practicals in Biology Education at UIN Jakarta Solihin, Solihin; Mailayfaiza, Septiandita; Ria, Ria; Syafkitamsana, Najwa; Harahap, Lia Junita; Giriyanti, Putri
Bioedunis Journal Vol 4, No 2 (2025)
Publisher : UIN Syekh Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/bioedunis.v4i2.18842

Abstract

Polymerase Chain Reaction (PCR) is a fundamental biotechnology technique used to amplify specific DNA fragments in large quantities through in vitro replication. This virtual laboratory aims to (1) understand the basic principles and working stages of PCR, (2) identify the main functions of PCR components, and (3) apply PCR simulations to amplify target genes using the LabXchange platform. The research was conducted virtually, using an interactive experimental approach, by 60 Biology Education students working in one group. Data were collected from student worksheets and simulation outputs. The results showed that students were able to identify and explain each stage of the PCR process, including denaturation, annealing, and extension, as well as analyze the roles of the DNA template, primers, dNTPs, buffer, and Taq polymeraseenzyme. The simulation successfully demonstrated efficient amplification of the target gene. These findings indicate that virtual laboratory learning effectively enhances students’ conceptual understanding and analytical skills in biotechnology.
Co-Authors Harahap, Lia Junita Mailayfaiza, Septiandita Ria, Ria Solihin Solihin Syafkitamsana, Najwa