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All Journal Jurnal Biologi Udayana
Yuli Yantika Syahputri
The integrated Laboratory, Poltekkes Kemenkes Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Evaluation of the potential of purple eggplant peel extract (Solanum melongena L.) as a pH indicator for Escherichia coli Wenni Oktraiani Purba; Yuli Yantika Syahputri; Nita Andriani Lubis; Gabriella Septiani Nasution; Dewi Setiawati; Febri Sembiring
Jurnal Biologi Udayana Vol. 29 No. 1 (2025): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2025.v29.i01.p01

Abstract

Anthocyanins are natural pigments found in various plants and fruits, known for their ability to change color according to the pH of their environment. This property can be utilized as a pH indicator in observing carbohydrate fermentation reactions by bacteria. This study aims to evaluate the potential of purple eggplant peel extract (Solanum melongena L.) as an alternative pH indicator to monitor bacterial growth. Purple eggplant peel was extracted using 80% ethanol containing 1% HCl, stored for 24 hours at 4 °C, centrifuged, and concentrated using a rotary evaporator at 35 °C. The extract obtained was used as a pH indicator in Tryptic Soy Broth (TSB) media for Escherichia coli growth. This test used two treatments; namely purple eggplant peel extract added into TSB media before bacterial inoculation and purple eggplant peel extract added after bacteria grew on TSB media. The results showed that purple eggplant peel extract effectively showed a clear color change according to different pH levels. However, when applied to bacterial growth media, the addition of purple eggplant peel extract showed no significant color change on TSB media, neither before nor after bacteria growth. Thus, to effectively utilize this extract as an indicator in bacterial growth media, further research is needed to explore methods to maintain the stability of the pigments contained in purple eggplant peel extract when added to bacterial growth media.
Co-Authors Dewi Setiawati Febri Sembiring Gabriella Septiani Nasution Nita Andriani Lubis Wenni Oktraiani Purba