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All Journal Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Biotek Spizaetus: Jurnal Biologi dan Pendidikan Biologi Sehat Rakyat: Jurnal Kesehatan Masyarakat Jurnal Biologi Udayana
Febri Sembiring
Politeknik Kesehatan Kementerian Kesehatan Medan
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IDENTIFIKASI BAKTERI Escherichia coli, Shigella sp. DAN Staphylococcus aureus SEBAGAI BIOMARKER CEMARAN PADA SUMBER AIR DAERAH STUNTING DI KELURAHAN BELAWAN SICANANG, KECAMATAN MEDAN BELAWAN, MEDAN Tania Regita Sari; Dinda Aulia Habib; Febri Sembiring; Gabriella Septiani Nasution; Lestari Rahmah
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3892

Abstract

Stunting is a disorder of growth and development of children due to chronic malnutrition and repeated infections, which are characterized by below standard height. Water is a kind of resource which required for community household activities,. Therefore, water, sanitation and hygiene become particular concern. Contamination by Enterotoxigenic Escherichia coli that transmitted through water can increase the risk of stunting in children with continuous diarrhea. This research was conducted to identify biomarkers in water sources for local residents with stunting in Belawan Sicanang Village, Medan. The biomarkers used were E. coli, Shigella sp., and Staphylococcus aureus. The method used in this study is the Most Probable Number (MPN) and Total Plate Count (TPC). The results of this study indicated that 2 out of 3 drinking water samples were contaminated with E. coli., and 3 out of 3 clean water samples were contaminated with E. coli. Meanwhile, the other two biomarkers were not detected in all types of samples.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli, Salmonella thypi, Staphylococcus aureus dan Staphylococcus epidermidis yuli yantika syahputri; Febri Sembiring; Gabriella Septiani Nasution; Nita Andriani Lubis
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3894

Abstract

Noni fruit (Morinda citrifolia L.) is one part of the Noni plant that contains metabolite compounds that are antibacterial. The antibacterial compounds produced are known to inhibit the growth of disease-causing bacteria. The purpose of this study was to measure the antibacterial activity of various concentrations of noni fruit extract against the growth of Escherichia coli, Salmonella thypi, Staphylococcus aureus and Staphylococcus epidermidis in vitro using the disc diffusion method. The results of this study indicate that the lowest concentration of Noni Fruit extract (60% v/v) can inhibit the growth of all types of bacteria tested. From the four test bacteria, the largest inhibition zone was found on Escherichia coli with a size of 23.05 mm at a concentration of 80% v/v Noni Fruit extract.
Pelayanan Pendaftaran Pasien Rawat Jalan di Rumah Sakit Elisabeth Medan Theresia Elen Novia Ziraluo; Agnes Louise Lasmaida; Febri Sembiring
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 3 No. 3 (2024): Agustus 2024
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v3i3.3088

Abstract

Abstract Hospital outpatient registration services are a phenomenon that refers to the actions or activities of a system within a hospital or health service facility which aims to maintain and improve health, prevent and cure disease and restore health. Outpatient care is a medical service provided to patients not in the form of inpatient care. This study aims to see an overview of outpatient registration services at Santa Elisabeth Hospital, Medan. This research uses a descriptive design with a quantitative approach. The sample in this study consisted of 30 patients. In this research, researchers collected data using a questionnaire. This research was conducted in June 2024. The data was analyzed univariably. The research results showed that there were 15 patients who received good and good service (50%), 10 patients received adequate service (33.33%) and 5 patients received poor service (16.66%). In this case, Santa Elisabeth Hospital Medan must continue to maintain good and friendly services and improve poor services.
Comparative Efficacy of Saga Seed-Derived Media in Supporting Staphylococcus aureus and Escherichia coli Growth Rahmah, Lestari; Noor, Vuan Sindra; Sari, Tania Regita; Putra, Geminsah; Sembiring, Febri
Jurnal Biotek Vol 13 No 1 (2025): JUNI
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v13i1.56606

Abstract

Saga seeds possess a high protein content, presenting a potentially economical substrate for microbiological medium development. This study evaluated the suitability of saga seeds (Adenanthera pavonina) as an alternative culture medium for propagating Escherichia coli and Staphylococcus aureus. The objective was to determine whether medium derived from saga seeds could sustain bacterial proliferation at levels comparable to conventional medium. It was postulated that both flour-based and infusion-based saga seed medium would yield bacterial growth statistically indistinguishable from that on Tryptic Soy Agar (TSA). Two formulation methods were examined: a flour-based medium and an infusion-based medium. E. coli and S. aureus were cultured on these alternative media and benchmarked against TSA. Colony-forming units (CFUs) were quantified and statistically analyzed using an independent samples t-test. The infusion-based medium displayed complete solubility with no residue, whereas the flour-based variant showed sedimentation. Colonies on both saga-based media appeared white, circular, and approximately 3 mm in diameter, consistent with those on the control medium. Based on productivity rate standards, both the saga seed medium fulfilled the minimum criteria for a non-selective medium. After adjusting for dilution, E. coli reached 1.06 × 10⁵ CFU/ml on the flour-based medium, exhibiting no statistically significant deviation from TSA. In contrast, the infusion-based medium supported lower E. coli growth at 0.94 × 10⁴ CFU/ml. S. aureus growth on the flour-based (1.08 × 10⁵ CFU/ml) and infusion-based medium (1.04 × 10⁵ CFU/ml) did not significantly differ from the control. The flour-based saga medium offers a promising and effective alternative for cultivating E. coli and S. aureus. Nonetheless, further optimization is advised to improve the flour-based formulation's solubility and visual clarity.
Evaluation of the potential of purple eggplant peel extract (Solanum melongena L.) as a pH indicator for Escherichia coli Wenni Oktraiani Purba; Yuli Yantika Syahputri; Nita Andriani Lubis; Gabriella Septiani Nasution; Dewi Setiawati; Febri Sembiring
Jurnal Biologi Udayana Vol. 29 No. 1 (2025): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2025.v29.i01.p01

Abstract

Anthocyanins are natural pigments found in various plants and fruits, known for their ability to change color according to the pH of their environment. This property can be utilized as a pH indicator in observing carbohydrate fermentation reactions by bacteria. This study aims to evaluate the potential of purple eggplant peel extract (Solanum melongena L.) as an alternative pH indicator to monitor bacterial growth. Purple eggplant peel was extracted using 80% ethanol containing 1% HCl, stored for 24 hours at 4 °C, centrifuged, and concentrated using a rotary evaporator at 35 °C. The extract obtained was used as a pH indicator in Tryptic Soy Broth (TSB) media for Escherichia coli growth. This test used two treatments; namely purple eggplant peel extract added into TSB media before bacterial inoculation and purple eggplant peel extract added after bacteria grew on TSB media. The results showed that purple eggplant peel extract effectively showed a clear color change according to different pH levels. However, when applied to bacterial growth media, the addition of purple eggplant peel extract showed no significant color change on TSB media, neither before nor after bacteria growth. Thus, to effectively utilize this extract as an indicator in bacterial growth media, further research is needed to explore methods to maintain the stability of the pigments contained in purple eggplant peel extract when added to bacterial growth media.
Evaluasi Tepung dan Infusum Kacang Hijau (Vigna radiata) sebagai Media Alternatif Pertumbuhan Staphylococcus aureus Febri Sembiring; Suparni; Dewi Setiyawati; Wardati Humaira; Sri Widia Ningsih; Nin Suharti; Astrid Siska Pratiwi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.727

Abstract

Limited access to and the high cost of commercial growth media have driven the development of alternative media based on locally available materials. Although several legume-based materials have been reported to support bacterial growth, the use of mung beans (Vigna radiata) as an alternative medium—particularly in the form of flour and infusion—and comparisons of their effectiveness against standard media remain limited. This study aims to evaluate the potential of these two forms as growth media for Staphylococcus aureus. This was an experimental study conducted from March to May 2024 at the Microbiology Laboratory of the Medan Public Health Polytechnic (Poltekkes Kemenkes). The method used was growth analysis via the Total Plate Count (TPC) method. The results showed that the average TPC of S. aureus on mung bean flour medium was 1.07 × 10⁷ CFU/mL, and on mung bean infusion medium it was 4.7 × 10⁶ CFU/mL, whereas TSA medium, as the standard medium, yielded 5.8Z× 10⁶ CFU/mL. This indicates that both forms of the mung bean alternative media were capable of supporting optimal bacterial growth, with the flour medium yielding a higher cell count compared to the standard medium (TSA). Morphologically, the colonies on the alternative media were smaller (±2 mm, yellowish-white) compared to those on TSA (±3 mm, yellow). Thus, it can be concluded that mung bean flour has the potential to be an effective alternative medium for growing S. aureus compared to the infusion medium, which is less optimal due to the possible degradation of nutrients during the heating process.
Co-Authors Agnes Louise Lasmaida Astrid Siska Pratiwi Dewi Setiawati Dewi Setiyawati Dinda Aulia Habib Gabriella Septiani Nasution Gabriella Septiani Nasution Lestari Rahmah Nin Suharti Nita Andriani Lubis Nita Andriani Lubis Noor, Vuan Sindra Putra, Geminsah Rahmah, Lestari Sari, Tania Regita Sri Widia Ningsih Suparni Tania Regita Sari Theresia Elen Novia Ziraluo Wardati Humaira Wenni Oktraiani Purba Yuli Yantika Syahputri yuli yantika syahputri