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All Journal Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical)
Joke L. Tombuku
Department of Biology, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Antibacterial Activity Test of Basil Leaves (Ocimum basilicum L.) Against Staphylococcus aureus Using the Diffusion Agar Method Selvana S. Tulandi; Joke L. Tombuku
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 8 No. 1 (2025): April 2025
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v8i1.499

Abstract

Infections caused by Staphylococcus aureus are among the leading causes of morbidity and mortality worldwide, particularly due to the increasing resistance to antibiotics. This study aims to evaluate the antibacterial activity of basil leaves (Ocimum basilicum L.) against S. aureus using the agar diffusion method. This laboratory experimental study used a completely randomized design: extract concentration treatments (100µL, 200µL, 300µL, 400µL), a positive control (synthetic antibiotic @amoxicillin), and a negative control (distilled water), each with three replications. The data were analyzed using non-parametric statistics, as the data were not normally distributed and lacked homogeneity. The Kruskal-Wallis ANOVA was used to determine differences between treatments. The results showed that basil leaf extract exhibited significant antibacterial activity, with all concentrations inhibiting the growth of S. aureus. The highest inhibition zone was observed at the 400µL concentration, with an average diameter of 9.28 ± 0.36 mm. These findings suggest the potential of basil leaves as a natural antibacterial source for developing alternative therapies against S. aureus infections.
Evaluation of the Effectiveness of Dragon Tail Leaf Extract (Rhaphidophora pinnata (Lf) Schott) on Incisional Wound Healing in White Rats (Rattus norvegicus) Joke L. Tombuku; Kristin Mosso; Jeane Mongi; Nerni O. Potalangi; Hanna M. Rumagit; Rahmat Santoso; Amal R. Ginting
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 8 No. 2 (2025): OKTOBER 2025
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v8i2.526

Abstract

Rhaphidophora pinnata (dragon tail leaf) has traditionally been used as a medicinal plant for treating wounds. This research aimed to evaluate the effectiveness of Rhaphidophora pinnata leaf extract on incision wound healing in male white rats (Rattus norvegicus). The study employed a laboratory experimental design using 15 rats divided into five treatment groups: negative control (aquades), positive control (povidone iodine), and topical extract concentrations of 15%, 20%, and 25%. Wound length was measured daily for 14 days. Statistical analysis using paired sample t-test showed a significant difference between pre-treatment and post-treatment wound conditions (p = 0.000 < 0.05; t = 5.391). The results demonstrated that all extract concentrations accelerated wound closure, with the 25% concentration showing the greatest healing effect and complete wound closure by day 14. These findings indicate that Rhaphidophora pinnata leaf extract is effective in promoting incision wound healing, particularly at a concentration of 25%.
Co-Authors Amal R. Ginting Hanna M. Rumagit Jeane Mongi Kristin Mosso Nerni O. Potalangi Rahmat Santoso Selvana S. Tulandi