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All Journal Jurnal Lantera Ilmiah Kesehatan
Vhiona, Clarissa Audhia
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Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Identification of Flavonoid Compounds in Ethanol Extract of Chromolaena odorata L. Leaves Using Infrared Spectrophotometry Ramadani, Suci; Vhiona, Clarissa Audhia; Ifvia; Ramadhani, Audi; Lifah, Musda; Fatmawati, Rahma
JURNAL LENTERA ILMIAH KESEHATAN Vol. 4 No. 1 (2026): Juni
Publisher : CV. Q2 Lantera Ilmiah Institut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52120/jlik.v4i1.105

Abstract

Flavonoids are an important group of secondary metabolites found in medicinal plants and possess various biological activities such as antioxidant, anti-inflammatory, and antibacterial properties. This study aims to identify and confirm the presence of flavonoid compounds in the ethanolic extract of Chromolaena odorata L. leaves using infrared spectrophotometry (FTIR) as a structural fingerprinting approach. Dried leaves were extracted by maceration using 90% ethanol, then the obtained extract was analyzed at a wavenumber range of 4000–400 cm⁻¹. FTIR results showed the presence of typical absorption of hydroxyl (–OH), carbonyl (C=O), aromatic double bonds (C=C), and ether (C–O–C) groups, which are the main characteristics of flavonoid structure. This spectral pattern is consistent with literature data and supporting phytochemical test results, thus confirming the role of FTIR as a qualitative confirmation tool and chemical fingerprint of flavonoid compounds in the ethanolic extract of C. odorata leaves. These findings strengthen the potential of this plant as a natural source of bioactive compounds for the development of standardized herbal medicines and further phytopharmaceutical research.
Co-Authors Fatmawati, Rahma Ifvia lifah, Musda Ramadani, Suci Ramadhani, Audi