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All Journal SEHATI: Jurnal Kesehatan Jurnal Biodiversitas dan Bioteknologi
Defi Nurul Hayati
Universitas Muhammadiyah Purwokerto
Health Professions Medicine & Pharmacology Nursing Public Health

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Hubungan Golongan Darah Orang Tua dan Anak dengan Metode Slide Sultan Rafi Pratama; Defi Nurul Hayati; Intan Nuraeni; Faesa Alya Fadillah; Regina Putri Nuari; Reva Rahma Sahara; Aliya Mar Atussalikhah; Griselda Clarissa Naila
SEHATI: Jurnal Kesehatan Vol 6, No 1 (2026)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/sehati.v6i1.154

Abstract

Blood group examination is an important procedure in blood transfusion practice to prevent fatal hemolytic reactions. The ABO and Rhesus blood group systems are genetically inherited following Mendelian laws. This study examined the relationship between parental blood groups and blood groups of Medical Laboratory Technology students at Muhammadiyah University of Purwokerto through slide method examination. A descriptive analytic design with a cross-sectional approach was used. The population was students using purposive sampling technique. Blood group examination was carried out using the slide agglutination method with antisera reagents A, B, AB, and D. Data were analyzed using Chi Square test with a significance level of 0.05. The distribution of student blood groups was found to be O 20 people (37.0%), B 18 people (33.3%), A 11 people (20.4%), and AB 5 people (9.3%). The Chi Square test results showed no significant relationship between father's blood group and students (p=0.080), but there was a significant relationship between mother's blood group and students (p=0.000). This study found that student blood groups are more influenced by mother's blood group than father's. It is recommended that students know their blood type as important medical data for blood transfusion and organ transplantation purposes.
Evaluasi Jumlah Leukosit, Jumlah Netrofil, dan Kadar Anti Streptolysin O pada Pasien Tonsilitis Anak Citra Nadia Salsabila; Dita Pratiwi Kusuma Wardani; Arif Mulyanto; Defi Nurul Hayati
Jurnal Biodiversitas dan Bioteknologi Vol 2 No 2 (2025): Biodiverse: Jurnal Biodiversitas dan Bioteknologi
Publisher : Jurnal Biodiversitas dan Bioteknologi

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Abstract

Tonsillitis is a common global disease that most frequently affects children, particularly those aged 5–9 years. The primary cause of tonsillitis is infection of the tonsils by Streptococcus ?-hemolyticus Group A bacteria. Leukocytes play a key role in fighting infections, while neutrophils, as the main cells involved in acute inflammation, destroy bacteria through phagocytosis. This study aimed to determine the correlation between leukocyte and neutrophil counts and Anti-Streptolysin O (ASO) levels in children with tonsillitis. This research employed a cross-sectional design involving 32 pediatric tonsillitis patients, both inpatients and outpatients, at Dr. R. Goeteng Taroenadibrata Regional Hospital, Purbalingga. ASO levels were measured using the latex agglutination method, while hematological parameters—total leukocyte and neutrophil counts—were measured using a hematology analyzer based on the flow cytometry method. Data on the correlation between leukocyte and neutrophil counts and ASO levels were analyzed using Spearman’s rho correlation test. The results showed no significant correlation between leukocyte count and ASO level (p = 0.424, r = 0.146), and no correlation between neutrophil count and ASO level (p = 0.653, r = 0.083). There was no significant correlation between leukocyte and neutrophil counts and ASO levels in children with tonsillitis. Further studies are recommended using ASO measurement methods such as ELISA or turbidimetry, as well as additional inflammatory markers including Interleukin-1 (IL-1), Interferon-? (IFN-?), and Tumor Necrosis Factor-? (TNF-?).
Co-Authors Aliya Mar Atussalikhah Arif Mulyanto Citra Nadia Salsabila Dita Pratiwi Kusuma Wardani Faesa Alya Fadillah Griselda Clarissa Naila Intan Nuraeni Regina Putri Nuari Reva Rahma Sahara Sultan Rafi Pratama