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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Kesehatan THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Surya Medika Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Meditory : The Journal of Medical Laboratory Herb-Medicine Journal The Indonesian Journal of Infectious Diseases Jurnal Media Analis Kesehatan Jurnal Labora Medika Jurnal Ilmu dan Teknologi Kesehatan Journal of Health Sciences Jurnal Kesehatan Jurnal Analis Medika Biosains (JAMBS) Medical Technology and Public Health Journal Jurnal Kesehatan BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Pena Medika : Jurnal Kesehatan International Islamic Medical Journal Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Prosiding Seminar Nasional Hasil-hasil Penelitian dan Pengabdian Pada Masyarakat Jurnal Kesehatan Cendikia Jenius Jurnal Kesehatan Jurnal Veteriner
Dita Pratiwi Kusuma Wardani
UNIVERSITAS MUHAMMADIYAH PURWOKERTO
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Analisi Kandungan Timbal (Pb) Pada Air Sumur Di Sekitar Tempat Pembuangan Akhir Kalipancur Kabupaten Purbalingga Dewi Kristiya Nugra Heny; Kurnia Ritma Dhanti; Dita Pratiwi Kusuma Wardani
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.224

Abstract

Air memiliki peran penting dalam memenuhi kebutuhan manusia sebagai sumber air minum. Salah satu penyebab pencemaran air adalah logam berat. Tujuan penelitian ini adalah menganalisis kandungan timbal (Pb) pada air sumur di sekitar TPA Kalipancur Kabupaten Purbalingga. Penelitian ini dilakukan secara observasional deskriptif pada bulan Agustus 2020 di Laboratorium Kimia Analisis dan Biologi Farmasi Fakultas Farmasi Universitas Muhammadiyah Purwokerto. Teknik pengambilan sampel dilakukan secara purposive sampling. Sebanyak 10 sampel air sumur diambil dengan radius 100-500 m dari TPA Kalipancur. Kadar timbal (Pb) diukur menggunakan metode Atomic Absorption Spectrophotometer (AAS) dan dianalisis secara deskriptif numerik. Hasil penelitian menunjukkan kandungan timbal (Pb) pada seluruh sampel air sumur di sekitar TPA Kalipancur Kabupaten Purbalingga sebesar <0,01 mg/L. 
Kadar IgE Penderita Alergi pada Mahasiswa Teknologi Laboratorium Medik Universitas Muhammadiyah Purwokerto Tantri Analisawati Sudarsono; Dita Pratiwi Kusuma Wardani; Arif Mulyanto
Jurnal Surya Medika (JSM) Vol 6 No 2 (2021): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v6i2.1952

Abstract

The prevalence of allergies is increasing from year to year due to the modern lifestyle in society. The incidence of allergies was influenced by several factors, such as genetics, age, sex, diet, food type, and also environmental factors. Determination of IgE levels in adolescents and young adults is important because the incidence of allergy is highly reported in adolescents and young adults. This study was conducted to determine IgE levels in allergies among university students in Universitas Muhammadiyah Purwokerto. This observational study was conducted among diploma students of Medical Laboratory Technology, Universitas Muhammadiyah Purwokerto. The data of characteristic subjects were collected by a self-administered questionnaire whereas data of IgE levels were collected by serum samples and measured by ELISA. A total of 23 students (4.35 % male and 95.65% female) were included. Seafood allergy (25%) were the commonest allergy among the students. Only 9% of subjects have combination allergies. The highest of IgE levels were 365 IU/mL.
Analisis Senyawa Klorin (Cl2) Pada Beras yang Dijual Di Pasar Sokaraja Kabupaten Banyumas Olivia Alfitri; Kurnia Ritma Dhanti; Dita Pratiwi Kusuma Wardani
Jurnal Kesehatan Vol 14, No.1 (2021): Jurnal Kesehatan
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jk.v14i1.12418

Abstract

Beras merupakan komponen penting dalam makanan sehari-hari untuk menunjang kebutuhan pokok. Masalah manipulasi mutu beras sudah sering  dilakukan oleh pedagang atau penggilingan beras. Penggunaan bahan pemutih pada dasarnya dilarang dalam bahan pangan. Tujuan penelitian ini untuk mengidentifikasi dan menentukan kadar klorin yang ada pada beras  yang dijual di Pasar Sokaraja Kabupaten Banyumas. Penelitian ini dilakukan secara observasional deskriptif. Data senyawa klorin dianalisis dengan uji univariat. Penelitian dilaksanakan pada bulan Maret 2020. Sampel penelitian berjumlah 24 sampel beras. Hasil analisis kualitatif menunjukkan sampel positif adanya reaksi presipitasi berupa endapan putih dan analisis kuantitatif secara Argentometri Mohr dengan titik akhir titrasi merah kecoklatan. Hasil analisis kualitatif menunjukkan bahwa 5 sampel (20,8%) beras positif mengandung klorin. Kadar klorin terendah yaitu 71,04 mg/L dan kadar klorin tertinggi sebesar 189,44 mg/L
Identifikasi Telur Soil Transmitted Helminths Pada Caisim (Brassica chinensis) di Pasar Patikraja Kabupaten Banyumas Nurul Qomariyah; Dita Pratiwi Kusuma Wardani; Retno Sulistiyowati
Herb-Medicine Journal: Terbitan Berkala Ilmiah Herbal, Kedokteran dan Kesehatan Vol 4, No 4 (2021): Herb-Medicine Journal Oktober 2021
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/hmj.v4i4.10448

Abstract

Caisim (Brassica chinensis) is one of the vegetables that has many benefits such as protein, carbohydrates, fat and plays an important role in meeting food needs, and is good for health. Caisim is well adapted to high temperatures and the price is relatively stable and easy to cultivate. The purpose of this study was to determine the presence of Soil-Transmitted Helminths eggs in caisim (B. chinensis). The research was conducted in a descriptive observational. The research was conducted in July-August 2020 at Parasitology and Microbiology Laboratory, Faculty of Medicine, Universitas Muhammadiyah Purwokerto. Identification of Soil-Transmitted Helminths eggs from 20 caisim as the samples used NaCl flotation method and were analyzed by using a univariate test with tabulated frequency distribution. The results showed that no Soil-Transmitted Helminths eggs were found in caisim (B. chinensis).   Caisim (Brassica chinensis) merupakan sayuran yang mempunyai banyak kandungan manfaat seperti protein,karbohidrat, lemak dan berperan penting untuk memenuhi kebutuhan pangan serta baik untuk kesehatan. Caisimmampu beradaptasi dengan baik terhadap suhu tinggi serta harga relatif stabil dan mudah diusahakan. Tujuanpenelitian ini untuk mengetahui adanya telur Soil Transmitted Helminths terdapat pada caisim (B. chinensis).Penelitian dilakukan secara observasional deskriptif. Penelitian dilakukan pada bulan Juli-Agustus 2020 diLaboratorium Parasitologi Fakultas Kedokteran Universitas Muhammadiyah Purwokwerto. Identifikasi telur SoilTransmitted Helminthsdari 20 sampel caisim dengan metode Flotasi NaCl. dianalisis dengan uji univariat dengandistribusi tabulasi frekuensi. Hasil penelitian menunjukan tidak ditemukan telur Soil Transmitted Helminths padacaisim (B. chinensis)
IDENTIFIKASI LARVA LALAT DALAM KEPENTINGAN POST MORTEM INTERVAL PADA BANGKAI TIKUS (Rattus novergicus) YANG DIBERI CIU OPLOSAN DI SCIENCE TECHNO PARK UNIVERSITAS MUHAMMADIYAH PURWOKERTO Dita Pratiwi Kusuma Wardani; Arif Mulyanto
Herb-Medicine Journal: Terbitan Berkala Ilmiah Herbal, Kedokteran dan Kesehatan Vol 2, No 1 (2019): Herb-Medicine Journal April 2019
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/hmj.v2i1.4204

Abstract

Ciu merupakan salah satu minuman tradisional Banyumas yang mengandung alkohol. Ciu dapat dibuat oplosan dengan bahan lain untuk meningkatkan cita rasa dan sensasi minuman, namun berdampak buruk bagi kesehatan bahkan dapat menyebabkan kematian. Serangga berperan penting dalam penentuan Post Mortem Interval. Adanya kandungan obat atau senyawa yang terdapat pada jenazah dapat mempengaruhi perkembangan larva lalat. Tujuan penelitian ini untuk mengetahui spesies larva lalat yang terdapat pada bangkai tikus yang diberi ciu oplosan. Penelitian dilakukan secara observasional deskriptif. Penelitian dilakukan pada bulan Juli-Agustus 2018.Sebanyak tiga ekor tikus Wistar diberi ciu yang dioplos dengan minuman berenergi, ditunggu hingga mati kemudian diletakkan di Science Techno Park Universitas Muhammadiyah Purwokerto. Larva yang diambil 10% dari total populasi yang ditemukan setiap harinya. Identifikasi larva melalui pembuatan preparat posterior spirakel. Data dianalisis dengan univariate. Larva Calliphora sp. (19,10%), Sarcophaga sp. (0,71%), Chrysomya megacephala (13,28%), dan Chrysomya bezziana (13,86%) teridentifikasi pada bangkai tikus yang diberi ciu oplosan di Science Techno Park Universitas Muhammadiyah Purwokerto. Kata Kunci: Ciu, Oplosan, Post Mortem Interval, Larva Lalat
Deteksi Endoparasit Cacing pada Hepar Tikus Laboratorium (Rattus norvegicus) dari Sentra Peternak di Kabupaten Banyumas dan Kabupaten Purbalingga Dita Pratiwi Kusuma Wardani; Kurnia Ritma Dhanti; Arif Mulyanto; Tantri Analisawati Sudarsono
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 17 Nomor 2 Desember 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v17i2.4713

Abstract

The presence of laboratory rats that are maintained and bred for laboratory purposes or laboratory observations is very necessary. The presence of endoparasite in laboratory rats will have an impact on the result of the research or laboratory observations. This study aims to detect helminth endoparasites in the liver of laboratory rats (Rattus norvegicus) from animal breeders in Banyumas and Purbalingga Districts. This research was an observational study with a cross-sectional design. A total of 52 laboratory rats were used in the study. Rats are killed with chloroform, liver surgery then identify the presence of worm larvae. Out of the 52 rats obtained, 7 (29.17%) from 24 laboratory rats in Banyumas District and 5 (17.86%) from 28 laboratory rats in Purbalingga District were infected with Taenia taeniaeformis. It is necessary to control helminth infections in laboratory rats, such as laboratory animal quarantine, health monitoring, and antihelmintic treatment. It is important to handle carefully during travel to assure the results of research or laboratory observations using the animals.
EFEK MONOSODIUM GLUTAMAT TERHADAP GAMBARAN HISTOLOGI HEPAR TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR Shely Ayu Lestari; Dita Pratiwi Kusuma Wardani; Tantri Analisawati Sudarsono
Jurnal Media Analis Kesehatan Vol 12, No 1 (2021): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v12i1.2000

Abstract

Monosodium glutamate (MSG) is a sodium salt of glutamic acid that naturally comes from vegetable and animal protein functioned as a taste enhancer. So far, the use of MSG in various countries has increased by 1 g / day and can have an impact on cell damage to several organs. The purpose of this study was to find out the effects of MSG on the liver histology of Wistar white rats (Rattus norvegicus). This study used Posttest Only Control Group Design consisting of 4 groups, as control and MSG treatment doses of 5 g / kg BW, 10 g / kg BW, and 15 g / kg BW for 14 days orally. Each group consisted of 6 male white rats (Rattus norvegicus) aged 2-3 months with a weight of 200-250 grams in good health. A day after 14 days of treatment, the rats' liver organs were taken and preparations were made using the paraffin method. Data were analyzed using the Kruskal-Wallis test. The results of research showed that the control group looked normal, while the P1 group had 40.5 (40-43)% cell abnormalities and 52.5 (27-56)% cell abnormalities. The P3 group showed the most significant liver cell damage, namely 60 (59-62)%. The results of the Kruskal-Wallis test analysis showed a significant difference of p = 0.034 between cell abnormalities and the dose level of MSG (p-value 0.05). It can be concluded that MSG administration in stratified doses can cause liver cell damage in the form of degeneration and necrosis. Giving a dose of MSG 15 g / kg BW for 14 days showed the most significant liver cell damage at 60 (59-62)%.Keywords: Cell Abnormality, Liver, Monosodium Glutamate
Determination Of IgE Levels In Allergies Among Medical Laboratory Technology Students In Universitas Muhammadiyah Purwokerto TANTRI ANALISAWATI SUDARSONO; DITA PRATIWI KUSUMA WARDANI; ARIF MULYANTO
Pena Medika Jurnal Kesehatan Vol 11, No 1 (2021): PENA MEDIKA JURNAL KESEHATAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/pmjk.v11i1.1259

Abstract

The prevalence of allergies is increasing from year to year due to the modern lifestyle in society. The incidence of allergies was influenced by several factors, such as genetics, age, sex, diet, food type, and also environmental factors. Determination Ig E levels in adolescents and young adults is important because the incidence of allergy is highly reported in adolescents and young adults. This study was conducted to determine Ig E levels in allergies among university students in Universitas Muhammadiyah Purwokerto This observational study was conducted among diploma students of Medical Laboratory Technology, Universitas Muhammadiyah Purwokerto. The data of characteristic subjects were collected by a self-administered questionnaire whereas data of Ig E levels were collected by serum samples and measured by ELISA. A total of 23 students (4,35 % male and 95,65% female) were included. Seafood allergy (25%) were the commonest allergy among the students. Only 9% of subjects have combination allergies. The highest of Ig E levels were 365 IU/mL.
EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum L.) DAN CUKA APEL TERHADAP MORTALITAS Pediculus humanus capitis Dina Aristya Purdiani; Dita Pratiwi Kusuma Wardani; Retno Sulistyowati
Jurnal Labora Medika Vol 5, No 1 (2021): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.185 KB) | DOI: 10.26714/jlabmed.5.1.2021.1-7

Abstract

Kutu rambut (Pediculus humanus capitis) merupakan parasityang hidup di rambut dan kulit kepala manusia dengan caramenghisap darah dari kulit kepala manusia. Penelitan inibertujuan untuk mengetahui efektivitas ekstrak bawangputih (Allium sativum L.) dan cuka apel terhadap mortalitasPh. capitis. Penelitian ini dilakukan secara trueeksperimental dengan rancangan posttest only control groupdesign yang terdiri dari 4 kelompok. Satu kelompok sebagaikontrol sedangkan 3 kelompok lainnya mendapat perlakuanpemberian ekstrak bawang putih konsentrasi 5%, cuka apelkonsentrasi 5%, kombinasi ekstrak bawang putihkonsentrasi 5% dan cuka konsentrasi 5%. Penelitiandianalisis dengan uji Kruskal-Wallis dan dilanjutkan denganuji lanjut Posthoc Mann Whitney U. Pemberian ekstrakbawang putih konsentrasi 5% berpotensi menyebabkanmortalitas Ph. capitis pada pengamatan menit ke-25.Pemberian cuka apel konsentrasi 5% berpotensimenyebabkan mortalitas Ph. capitis pada pengamatan menitke-15. Pemberian kombinasi ekstrak bawang putihkonsentrasi 5% dan cuka apel konsentrasi 5% mampumenyebabkan mortalitas Ph. capitis pada pengamatan menitke-10 bila dibandingkan dengan kelompok kontrol,kelompok ekstrak bawang putih konsentrasi 5%, dan cukaapel konsentrasi 5%. Terdapat perbandingan pemberianekstrak bawang putih konsentrasi 5% dan cuka apelkonsentrasi 5% berpotensi menyebabkan mortalitas Ph.capitis. Kombinasi ekstrak bawang putih konsentrasi 5%dan cuka apel konsentrasi 5% lebih efektif dalammembunuh Ph. capitis.
DETEKSI KEBERADAAN TELUR Soil Transmitted Helminth (STH) PADA KUKU PETANI DITA PRATIWI KUSUMA WARDANI
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1698

Abstract

Soil-Transmitted Helminth (STH) is an intestinal nematode that requires soil for the process of reproduction and maturation from non-infective to infective. The nematodes included in this species include Ascaris lumbricoides, Trichuris trichiura, and two species of hookworm, Necator americanus, and Ancylostoma duodenale. Personal hygiene can affect the incidence of helminth infections in humans. The purpose of this study was to determine the relationship between personal hygiene and the presence of Soil-Transmitted Helminth (STH) eggs on farmers' nails. This research was conducted by analytical observation with a cross-sectional design in April-May 2021. The research method was filling out questionnaires and identifying eggs using the 10% KOH sedimentation method. Data were analyzed by univariate and Pearson Chi-Square tests. The results of this study showed that 4 fingernail samples (47.05%) were positive for Ascaris lumbricoides eggs and 2 fingernail samples (11.76%) were positive for Minute Intestinal Fluke, as many as 8 toenail samples (82.35%) were positive for Ascaris lumbricoides eggs. and 1 sample of toenails (5.88%) positive for Minute Intestinal Fluke eggs. There was no relationship between personal hygiene and the presence of STH eggs on farmers' nails (p = 0.05). STH eggs were found in the fingernails and toenails of farmers in Kaligondang District, Purbalingga Regency. Farmers need to improve personal hygiene to avoid helminth infections.
Co-Authors Almanfaluthi, Muhammad Lutfhi Amalia Ovi Mustika Seno Ambarwati, Dewi Annisa Nur Chaeni Aprianto . Arif Mulyanto Arif Mulyanto Arif Mulyanto, Arif ATIKA NUR AZIZAH Atika Nur Azizah Cahyaningrum, Winda Rahma Chaeni, Annisa Nur Dewi Kristiya Nugra Heny Dhanti, Kurnia Ritma Dhanti, Kurnia Ritma Dina Aristya Purdiani EKANIASARI, MEVIANA DEA Farida Dwi Handayani Firman Jaelani HAMNA FATHIA ZAHRA Handayani Narendo Putri handayani, Farida Heny, Dewi Kristiya Nugra Ika Wulan Nuri Anggreani Ikhsan Mujahid Ikhsan Mujahid Ikhsan Mujahid IKHSAN MUJAHID Indah Mulyani Ismi Andina Pertiwi ISNA HIKMAWATI Isna Hikmawati Isna Hikmawati Janatin Nur Aripin JANG JAYA, NABELA PUTRI Ketut Tirtayasa Kurnia Ritma Dhanti Kurniawan Kurniawan Kurniawan Linda Wijayanti Listiyowati, Tanti M Luthfi Almanfaluthi M. Luthfi Almanfaluthi Mika'il, Hikam Minto Rahaju Minto Rahaju Muhammad Lutfhi Almanfaluthi Muhammad Luthfi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi Muhammad Luthfi Almanfaluthi MUJIONO, TIMBUL Nadia Ega Haliza Nuri Anggreani, Ika Wulan Nurul Badriyah Nurul Badriyah Nurul Qomariyah Oei Stefani Yuanita Widodo OEI STEFANI YUANITA WIDODO Oktafian Diyas Tamtama Olivia Alfitri Qo'imah, Alamanda Nurul Qo’imah, Alamanda Nurul Rahaju, Minto Retno Sulistiyowati RETNO SULISTIYOWATI RETNO SULISTYOWATI Retno Sulistyowati Retno Sulistyowati Ristiyanto Ristiyanto Ristiyanto ROHMAH, JAZILATUR Santi Safitri Shely Ayu Lestari SUDARSONO, TANTRI ANALISAWATI Sudarsono, Tantri Analisawati Supriyadi Supriyadi Supriyadi Syafira Dian Ergiana Syifa Arrachmah Tantowi, Linda Yanuana Fanida Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Tantri Analisawati Sudarsono Wijayanti, Linda Yunita Nurmalasari