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Perbandingan Hasil Pemeriksaan Hitung Jumlah Eritrosit Menggunakan EDTA Konvensional dan Vacutainer Lilis Septiana Dewi; Tantri Analisawati Sudarsono; Retno Sulistiyowati
Jurnal Surya Medika (JSM) Vol 7 No 2 (2022): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v7i2.2842

Abstract

Hematology examination in practice often uses anticoagulants to prevent clotting in blood samples, including in the examination of the erythrocyte count. The anticoagulant that is often used for examination is EDTA (Ethylene Diamine Tetra Acetic Acid). With the changing times, the addition of anticoagulants to the examination is no longer done conventionally but is already contained in the vacutainer tube. However, the vacutainer anticoagulant cannot be adjusted to the blood volume obtained during phlebotomy, while the conventional anticoagulant can be added according to the blood volume obtained. This research is a pre-experimental research design with a One-Shot Case Study design, in which each blood sample is added with conventional anticoagulants and vacutainers. The method used is manual using a hemocytometer and a microscope. Analysis of the data using the Independent T-test. The purpose of this study was to find out the comparison of the results of the erythrocyte count using conventional EDTA and a vacutainer. After examination with both anticoagulants, p-value = 0.822 (p>0.05) so there is no comparison. Based on these results, it can be concluded that there is no comparison of erythrocyte count examination using conventional EDTA and vacutainer.
Analisis Kadar Rhodamin B pada Terasi yang Diperjualbelikan di Pasar Belik Kabupaten Pemalang Oden Krisyan; Retno Sulistiyowati; Kurniawan Kurniawan
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2143

Abstract

Terasi is a cooking spice made from fermented shrimp and is often found in the market. It is relatively affordable and easy to find, so people flavor it as a flavor enhancer for their food. The abuse of Rhodamine B as a Food Additive (BTP) in terasi is often done by the producers. This study aimed to analyze the Rhodamine B content in the terasi sold at the Pasar Belik, Pemalang Regency. The study was conducted in a descriptive observational manner where the sample was analyzed qualitatively, and if the result were positive, it would be followed by quantitative. The study was conducted in December 2020. Rhodamine B content in terasi was analyzed by univariate test. Qualitative analysis was carried out by chemical methods using 10% NaOH, Petroleum Ether, and 10% HCl. The qualitative analysis of 7 samples of shrimp paste sold at the Belik Market in Pemalang Regency were all negative and did not contain Rhodamine B. Shrimp samples that were bought and sold at the Pasar Belik in Pemalang Regency showed that they were free from Rhodamine B.
Identifikasi Telur Soil Transmitted Helminths Pada Caisim (Brassica chinensis) di Pasar Patikraja Kabupaten Banyumas Nurul Qomariyah; Dita Pratiwi Kusuma Wardani; Retno Sulistiyowati
Herb-Medicine Journal: Terbitan Berkala Ilmiah Herbal, Kedokteran dan Kesehatan Vol 4, No 4 (2021): Herb-Medicine Journal Oktober 2021
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/hmj.v4i4.10448

Abstract

Caisim (Brassica chinensis) is one of the vegetables that has many benefits such as protein, carbohydrates, fat and plays an important role in meeting food needs, and is good for health. Caisim is well adapted to high temperatures and the price is relatively stable and easy to cultivate. The purpose of this study was to determine the presence of Soil-Transmitted Helminths eggs in caisim (B. chinensis). The research was conducted in a descriptive observational. The research was conducted in July-August 2020 at Parasitology and Microbiology Laboratory, Faculty of Medicine, Universitas Muhammadiyah Purwokerto. Identification of Soil-Transmitted Helminths eggs from 20 caisim as the samples used NaCl flotation method and were analyzed by using a univariate test with tabulated frequency distribution. The results showed that no Soil-Transmitted Helminths eggs were found in caisim (B. chinensis).   Caisim (Brassica chinensis) merupakan sayuran yang mempunyai banyak kandungan manfaat seperti protein,karbohidrat, lemak dan berperan penting untuk memenuhi kebutuhan pangan serta baik untuk kesehatan. Caisimmampu beradaptasi dengan baik terhadap suhu tinggi serta harga relatif stabil dan mudah diusahakan. Tujuanpenelitian ini untuk mengetahui adanya telur Soil Transmitted Helminths terdapat pada caisim (B. chinensis).Penelitian dilakukan secara observasional deskriptif. Penelitian dilakukan pada bulan Juli-Agustus 2020 diLaboratorium Parasitologi Fakultas Kedokteran Universitas Muhammadiyah Purwokwerto. Identifikasi telur SoilTransmitted Helminthsdari 20 sampel caisim dengan metode Flotasi NaCl. dianalisis dengan uji univariat dengandistribusi tabulasi frekuensi. Hasil penelitian menunjukan tidak ditemukan telur Soil Transmitted Helminths padacaisim (B. chinensis)
The Correlation between Blood Glucose Levels, Body Mass Index (BMI), and Age on Prolanis Patients in Baturraden Public Health Care Center II Sri Yuliati; Retno Sulistiyowati; Umi Solikhah; Kurnia Ritma Dhanti
Science Midwifery Vol 10 No 3 (2022): August: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i3.618

Abstract

Health services through the chronic disease management program or Prolanis is a process to determine the health condition of patients with chronic diseases regularly, glucose being one of the parameters of the examination. Glucose levels are influenced by several factors, which are age and body mass index. This study aims to determine the relationship between blood glucose levels with body mass index and age in prolanis patients at Baturraden II Health Center. This type of research is a quantitative study with a cross sectional design using a total sampling technique and the GOD-PAP (Glucose Oxidase Para Amino Peroxidase) examination method on prolanis patients at Baturraden II Health Center. The research was carried out in April 2022 and obtained the results of the examination of blood glucose levels with an average of 133.30 mg/dl, the relationship between age and body mass index respectively to blood glucose levels was p value = 0.701 and p value = 0.211. So it can be concluded that there is no significant relationship between blood glucose levels with body mass index and age in prolanis patients at Baturraden II Health Center.
The Comparison of Blood Glucose Check Results Using Photometer and Glucometer Iswarno; Retno Sulistiyowati; Kurnia Ritma Dhanti; Tantri Analisawati
Science Midwifery Vol 10 No 3 (2022): August: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i3.653

Abstract

Diabetes mellitus is a disease caused by high blood glucose levels. Blood glucose is a sugar that functions as an energy source for humans, formed from carbohydrates consumed and then stored as glycogen in the liver and muscles. Examining blood glucose levels can be done in two ways, namely using a photometer and a glucometer. This study aimed to determine differences in the results of blood glucose checks measured using a photometer and glucometer. The research was conducted with an analytic observational design with a cross-sectional approach. The study was conducted in June 2022. The population in this study were all Prolanis participants at the Klirong 1 Public Health Care Center. The sampling technique in this study used the accidental sampling technique. The data analysis used paired sample T-test. The results of the blood glucose checks using a photometer and glucometer showed a p-value (0.000) and alpha value (0.05). So it can be concluded that there is a significant difference between the results of blood glucose checks using a photometer and a glucometer.
The Correlation Between Uric Acid Levels (Photometer Method) And The Incidence of Hypertension in Prolanis Patients at Puskesmas Klirong II Kebumen Regency Budi Setyawan; Retno Sulistiyowati; Umi Solikhah
Science Midwifery Vol 10 No 3 (2022): August: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i3.664

Abstract

Health service through Prolanis (Program Pengelolaan Penyakit Kronis or Chronic Disease Management Program in English) is a management program for health maintenance in order to achieve an optimal quality of life. One of the targets of prolanis is patients with hypertension. Hypertension can cause the blockage of uric acid crystals in blood vessels so that kidney function is disrupted. As a result, there is an increase in uric acid levels. Based on a preliminary study conducted on March 14, 2022, at Puskesmas Klirong II, it was found that 23 prolanis patients had complaints of joint pain, while 38 of them had hypertension. This research aimed to find out whether there is a correlation between uric acid levels and the incidence of hypertension in prolanis patients at Puskesmas Klirong II. The population was 50 people with a sample of 37 people. The sample was obtained by employing random sampling. This was an Analytical Observational research with a Cross-Sectional research design. The uric acid levels were checked with the Urikase-PAP photometer method. This research was conducted in May 2022. The results showed that 4 respondents had normal uric acid levels and normal blood pressure. On the other hand, there were 12 respondents who had normal uric acid levels and high blood pressure. Meanwhile, the other 21 respondents had high uric acid levels and high blood pressure. The results of the Fisher's Exact Test statistical test obtained a p-value = 0.028 <0.05. It was concluded that there was a correlation between uric acid levels (photometer method) and the incidence of hypertension in prolanis patients at Puskesmas Klirong II, Kabupaten Kebumen.
Factors Associated with Hindrance to Lowering Blood Pressure in Hypertensive Prolanis Patients at Ajibarang I Public Health Care Center Banyumas Regency Sovi Haswindha Putri Nugraheni; Retno Sulistiyowati; Umi Solikhah; Kurnia Ritma Dhanti
Science Midwifery Vol 10 No 3 (2022): August: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i3.672

Abstract

Hypertension is a condition of abnormal and continuous increasing blood pressure, which has been checked several times. It is caused by some risk factors so that blood pressure cannot be maintained properly. The risk factors for hypertension that cannot be changed include age, gender, and genetics. Modifiable risk factors include smoking, a low fiber diet, dyslipidemia, and excessive salt consumption. In particular, dyslipidemia is a metabolic disorder characterized by an increase in total cholesterol, LDL, triglycerides, and HDL. Individuals who suffer from hypertension must receive regular care. One is by following Prolanis, so their high blood pressure does not cause disease complications. This study aimed to determine what factors were associated with hindrance to lowering blood pressure and the relationship of HDL levels and physical activity with blood pressure in patients with hypertension Prolanis at Ajibarang Public Health Center I. This quantitative research utilized the total sampling technique and the method of examining HDL levels of precipitation. The study was conducted in June 2022. It is shown that the relationship between HDL levels with systolic blood pressure and diastolic blood pressure were p = 0.870 and p = 0.870, respectively. Meanwhile, the relationship between physical activity and systolic blood pressure and diastolic blood pressure were p = 0.055 and p = 0.754, respectively. It meant that there was no relationship between HDL levels and physical activity with blood pressure in hypertension Prolanis patients at Ajiabarang Public Health Center I.
HUBUNGAN LAMA KONSUMSI MINUMAN BERALKOHOL DENGAN KADAR SGOT DAN SGPT DI DESA KEMOJING KECAMATAN BINANGUN KABUPATEN CILACAP Falih Aziz Nurzhorif; Retno Sulistiyowati
Jurnal Keperawatan dan Kesehatan Masyarakat Cendekia Utama Vol 11, No 3 (2022): Jurnal Keperawatan dan Kesehatan Masyarakat Cendekia Utama
Publisher : STIKES Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31596/jcu.v11i3.985

Abstract

ABSTRAK Minuman beralkohol adalah minuman yang mengandung alkohol dengan kadar yang berbeda-beda. Penyakit akibat mengonsumsi alkohol salah satunya adalah gangguan fungsi hati. Pemeriksaan yang umum dilakukan untuk mengetahui fungsi hati yaitu pengukuran kadar SGOT dan SGPT. Tujuan dari penelitian ini adalah mengetahui hubungan lama konsumsi minuman beralkohol dengan kadar SGOT dan SGPT di Desa Kemojing Kecamatan Binangun Kabupaten Cilacap yaitu sejumlah 32 orang. Penelitian berjenis observasional analitik dengan rancangan cross sectional dan teknik pengambilan sampel purposive sampling. Data dianalisis dengan uji Chi Square. Hasil dari penelitian ini diperoleh rata-rata kadar SGOT 21.072 U/L dan rata-rata kadar SGPT 22.600 U/L. Dengan uji Chi Square diperoleh nilai P>0.05 pada kadar SGOT, dan nilai P<0.05 pada kadar SGPT. Penelitian dapat disimpulkan bahwa tidak terdapat hubungan signifikan lama konsumsi minuman beralkohol dengan kadar SGOT dan terdapat hubungan signifikan lama konsumsi minuman beralkohol dengan kadar SGPT.Kata Kunci: Alkohol, Lama konsumsi, SGOT, SGPT   ABSTRACT Alcoholic beverages are beverages that contain alcohol in varying degrees. Impaired liver function is one of the disorders caused by alcohol consumption. Common tests performed to determine liver function are the measurement of SGOT and SGPT levels. The purpose of this study was to determine the relationship between the duration of consumption of alcoholic beverages and levels of SGOT and SGPT in Kemojing Village, Binangun                             nSub-District, Cilacap Regency. This study involved 32 people who were selected using purposive sampling technique. This was an analytic observational study with a cross sectional design. Afterwards, the data were analyzed by using Chi Square test. The result showed that the average level of SGOT and SGPT were 21.072 U/L and 22.600 U/L, respectively. In terms of Chi Square test, the value of SGOT level was P>0.05, and the value of SGPT level was P<0.05. Thus, it could be concluded that there was no significant relationship between the duration of consumption of alcoholic beverages and levels of SGOT. Meanwhile, there was a significant relationship between the duration of consumption of alcoholic beverages and levels of SGPT.Keywords: Alcohol, Duration of consumption, SGOT, SGPT
PENGENDALIAN KADAR KOLESTEROL SEBAGAI UPAYA PENEKANAN PENYAKIT JANTUNG KORONER PADA ANGGOTA AISYIYAH DESA KEBANGGAN KEC SUMBANG Kurnia Ritma Dhanti; Retno Sulistiyowati
PROSIDING SEMINAR NASIONAL LPPM UMP Vol 1 (2019): PROSIDING SEMINAR NASIONAL LPPM UMP 2019
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.004 KB)

Abstract

Warga Aisyiyah desa Kebanggan kecamatan Sumbang mayoritas bekerja sebagai ibu rumahtangga. Seperti halnya ibu rumah tangga lainnya, kecenderungan untuk hidup hemat terkadang melalaikan resiko bahaya yang bisa mengintai, salah satu contohnya adalah penggunaan minyak goreng secara berulang demi alasan ekonomis. Padahal minyak yang dipanaskan berkali-kali dapat mengalami perubahan struktur menjadi senyawa yang berbahaya. Berdasarkan hasil penelitian dari tim penyuluh, cincau diketahui dapat menurunkan bilangan peroksida, sehingga dapat menurunkan resiko bahaya jelantah. Pada kegiatan ini, dilakukan sosialisasi penggunaan cincau untuk menurunkan resiko bahaya jelantah. Selain pemaparan tersebut, tim penyuluh juga memberikan materi tentang pemanfaatan jelantah sebagai bahan dasar pembuatan lilin. Penyuluhan ini diharapkan warga Aisyiyah desa Kebanggan dapat lebih bijak dalam menggunakan jelantah. Pengurangan pemakaian jelantah secara tidak langsung dapat membantu mengendalikan terjadinya penyakit akibat konsumsi kolesterol berlebih, seperti jantung koroner.
PENGARUH PEMBERIAN AIR REBUSAN DAUN SALAM (Syzygium polyanthum Wight) TERHADAP KADAR ASAM URAT PADA TIKUS PUTIH JANTAN (Rattus norvegicus) Retno Sulistiyowati; Neneng Siti Nurngaisah
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 17 No. 1 (2022): Jurnal Ilmiah PANNMED Periode januari -April 2022
Publisher : Poltekkes Kemenkes Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.267 KB) | DOI: 10.36911/pannmed.v17i1.1239

Abstract

Daun salam (Syzygium polyanthum Wight) merupakan tumbuhan yang sering dimanfaatkan sebagai penyedap rasa. Kandungan daun salam secara ilmiah diketahui yaitu flavonoid, tanin dan minyak atsiri yang mampu menurunkan kadar asam urat. Asam urat merupakan sisa-sisa pembuangan bahan makanan yang mengandung nukleotida purin atau nukleotida purin yang berasal dari tubuh. Tujuan penelitian adalah untuk mengetahui pengaruh pemberian air rebusan daun salam (Syzygium polyanthum Wight) terhadap kadar asam urat pada tikus putih jantan (Rattus norvegicus). Jenis penelitian ini adalah quasi eksperimental dengan metode total sampling dengan jumlah sampel sebanyak 20 ekor tikus. Analisis statistik menggunakan uji T-test dependent untuk mengetahui adanya pengaruh air rebusan daun salam terhadap kadar asam urat pada tikus putih jantan (Rattus norvegicus). Hasil uji T-test dependent menunjukan hasil yang signifikan p=0,000 (p< 0,05). Berdasarkan hasil penelitian, dapat disimpulkan menunjukan adanya penurunan kadar asam urat pada tikus putih jantan setelah pemberian air rebusan daun salam.