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All Journal International Journal of Health, Engineering and Technology
Ambararum, Tiara Rizki
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Dentistry Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Comparison of Antioxidant Activity of Sheet Mask Ethanol Extract of Kesum Leaves (Polygonum Minus H) Using DPPH and FRAP Methods Ambararum, Tiara Rizki; Annie Rahmatillah; Vivin Marwiyati Rohmana
International Journal of Health Engineering and Technology Vol. 4 No. 6 (2026): IJHET MARCH 2026
Publisher : CV. AFDIFAL MAJU BERKAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55227/ijhet.v4i6.674

Abstract

Skin aging due to oxidative stress from free radicals requires natural antioxidants such as kesum leaves (Polygonum minus Huds.) which are rich in flavonoids and phenolics. This study aims to formulate a sheet mask from ethanol extract of kesum leaves at a concentration of 1-3%, evaluate the physicochemical properties, and compare antioxidant activity with DPPH and FRAP methods. Quantitative experimental research with a comparative design used purposive sampling of kesum extracts and formulations (F0-F3), with three replications per concentration (a total of 12 samples). Instruments include a UV-Vis spectrophotometer (DPPH 517 nm, FRAP 593 nm), pH meter, viscometer; linear regression data analysis for IC₅₀ and t/ANOVA test. The results showed that the IC₅₀ extract DPPH 38.12 ppm (very strong) and FRAP 67.37 ppm (strong); F3 sheet mask optimal IC₅₀ DPPH 41.59 ppm, pH 5.43, viscosity 385 mPa.s, spreadability 6.7 cm, non-irritant, increased humidity 50-60%. The DPPH method is more sensitive than FRAP. In conclusion, F3 sheet mask has the potential to be a strong antioxidant for anti-aging cosmetics, in vivo and stability tests are recommended.
Co-Authors Rahmatillah, Annie Rohmana, Vivin Marwiyati