Purpose: A vaccine is a biological product that contains microorganisms that have been weakened or inactivated, but still have intact parts, or that have been modified into recombinant proteins, which are then combined with other substances. Vaccine storage involves the proper handling of received vaccines to ensure their safety and protect them from physical damage, thereby maintaining their quality according to established requirements until they are used. This study aimed to evaluate vaccine storage and quality in independent midwife’s practice. Methodology: This study was a cross-sectional quantitative study. It was conducted at the Independent Midwife Practice (IMP) of the Muntilan 1 Public Health Center in the period of July 2024. Vaccine storage and quality were evaluated based on vaccine management guidelines in healthcare facilities. Results: Vaccine storage at several Independent Midwives’ Practices (IMPs) in the working area of Muntilan 1 Public Health Center was inadequate. Based on the study results, the average storage compliance percentages at several IMPs were 83.3%, 50%, and 33.3%, respectively. Meanwhile, the average vaccine quality percentages were 100%, 96.8%, and 100%. Conclusions: Vaccine storage practices at independent midwives’ practices were not fully compliant with national guidelines, although overall vaccine quality remained good based on VVM status, expiration checks, and shake tests. Strengthening monitoring and improving storage procedures are needed to ensure consistent vaccine safety. Limitations: Some indicators are not relevant to each IMP; therefore, the indicators are not included in the research criteria. Contributions: This study can help people in charge of storage, especially midwives in independent practices, improve vaccine quality.