<![CDATA[This study aims to evaluate the effect of forest honey on fibroblast proliferation and migration under hyperglycemic conditions in vitro. The research method involved culturing primary fibroblasts in high-glucose DMEM (25 mM) and dividing them into five groups: standard control (standard medium), hyperglycemia control (high-glucose medium without treatment), 3% honey, 1.5% honey, and 0.75% honey. Proliferation was assessed by counting live cells (Trypan Blue staining/hemocytometer) at 24, 48, and 72 hours. Migration was measured using a scratch assay (0, 24, 48, 72 hours) and analyzed with ImageJ. The Shapiro–Wilk test was used for normality; data were analyzed with ANOVA followed by LSD or Kruskal–Wallis (p<0.05). Results showed that hyperglycemia decreased proliferation compared to the standard control. Treatment with 1.5% honey and 0.75% honey consistently increased proliferation compared to the hyperglycemic control at all time points (p<0.05), while 3% honey showed a relatively lower increase. The standard control group achieved 100% closure at 72 hours in the migration variable. The honey groups (0.75–3%) showed an increase compared to the hyperglycemic control, but the difference was not significant at 24–48 hours and approached significance at 72 hours (p=0.057). In conclusion, forest honey at a concentration of 1.5% can increase fibroblast proliferation in a hyperglycemic environment. The effect on migration requires confirmation with osmolality controls and more sensitive endpoints. These findings provide a biological basis for developing honey-based diabetic wound care adjuvants focusing on dose optimization and formulation standardization.]]>
