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All Journal African Multidisciplinary Journal of Sciences and Artificial Intelligence Kwaghe International Journal of Sciences and Technology
Patience Audu Jankada
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Social Sciences

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The Total Antioxidant Levels, Amino Acids, Vitamins, Flavonoids and Phenolics Profile of Persea americana Using HPLC Ojochenemi Ejeh Yakubu; Janya Danjuma; Patience Audu Jankada
African Multidisciplinary Journal of Sciences and Artificial Intelligence Vol 1 No 2 (2024): African Multidisciplinary Journal of Sciences and Artificial Intelligence
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/amjsai.v1i2.3864

Abstract

This research investigated the antioxidant levels, flavonoids and phenolics, amino acid and vitamin profile of P. americana seed methanol extract. Fresh fruits of P. americana were procured from Baissa in Taraba State. The fruits were cut open and the seed removed, washed and sliced. The plant material was air dried at room temperature after which it was pulverized and extraction was carried out using methanol for 72 hours. Extract was subjected to total antioxidant test using DPPH by serial dilution. Different portions of the extract were used to analyze for total flavonoid content and total phenolics content using aluminium chloride and folin-ciocalteu respectively. Furthermore, a portion of the extract was used to analyze for amino acid, vitamin and flavonoids and phenolics profile. The result reveals that the portion antioxidant activity increase with concentration. The portion with 1000µg/1000mL had over 80% which was the highest inhibition percentage in the series. Total flavonoid was found to be 39.88 ± 0.44 while the total phenolic content was seen to be 35.84 ± 4.30. Quercetin and chlorogenic acid were seen to be present for HPLC analysis of flavonoids and phenolics profile to be 15.8% and 84.2% respectively. The result for vitamin profile reveals that three vitamins were detected: vitamins K, B1 and B3 at 0.1%, 20.4% and 79.5% respectively. The amino acid present include asparagine, threonine, serine and aspartic acid with 1.1%, 13.9%, 10.4 and 74.6% abundance respectively. This shows how rich how this plant material can be and also reveals the hidden potentials inherent in it. This could be used to supplement diets and meet various nutritional and needs of individuals.
Determination of the Total Phenolics, Flavonoids, Phenolics and Flavonoids Profile, Vitamins and Amino Acids Profile Using HPLC and DPPH Free Scavenging Radical Acitivities of the Methanol Leaf Extract of Persea americana Ojochenemi Ejeh Yakubu; Olawale Otitoju; Janya Danjuma; Patience Audu Jankada
Kwaghe International Journal of Sciences and Technology Vol 1 No 1 (2024): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v1i1.3571

Abstract

This research examined the total phenolics, flavonoids, flavonoids and phenolics profile, vitamins and amino acids profile uing HPLC as well as the DPPH free scavenging activity of methanol leaf extract of P. americana. Plants have shown to possess phytochemicals which are beneficial to man including therapeutic and disease prevention. P. americana leaves was harvested from its tree in Baissa, Taraba State. The leaves were air dried under shade at room temperature after which it was pulverized using clean mortar and pestle. The powdered sample was extracted using methanol for 72 hours. Extract was filtered using whatmann no. 1 filter paper and evaporated using rotary evaporator and water bath to obtain the required concentrate. The concentrated extract was subjected to total flavonoid and total phenolics content using aluminium chloride folin ciocaltue respectively. Total antioxidant capacity of the leaf extract was carried out using DPPH. The amino acids, vitamins, flavonoids and phenolics profiles were evaluated. The result reveals that there is a significant level of flavonoid and phenolics in the extract which accounted for 43.32±0.15 mg GAE/100g and 32.84±2.13 mg CE/100g respectively. The antioxidant capacity showed a promising inhibition of DPPH free radical scavenging activity with increase in dose. The amino acids that were found to be present in the extract include asparagine, threonine, histidine, phenylalanine and aspartic acid with different percentage. Vitamins K, B1 and B3 were detected significantly in the leaf extract. The various phytochemicals detected have shown according to research to have beneficial to the biological system, especially in scavenging free radicals and mitigation of oxidative stress. Vitamins are essential for normal functions and metabolism in the biological system, their deficiency could have deleterious effects. Amino acids are necessary for protein synthesis and are required for other biological processes. The extract has shown to be a rich source of various phytochemicals and have shown a promising presence of various vitamins and amino acids.
Co-Authors Janya Danjuma Ojochenemi Ejeh Yakubu Olawale Otitoju