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All Journal MEDULA Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo
Sufiah Asri Mulyawati
Halu Oleo University
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The Antibacterial Activity of Arabian Bidara Leave (Ziziphus spina-christi L.) against the growth of Escherichia coli Sufiah Asri Mulyawati; Amiruddin Eso; Yusni Nurul Hidayah
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 11 No. 2 (2024)
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46496/medula.v11i2.38

Abstract

ABSTRACT Background: The primary cause of mortality for children, according to the World Health Organization, is infectious illnesses. Escherichia coli is one of the bacteria that may infect people. Infections caused by E. coli have historically been treated with antibiotics. Antibiotic resistance, however, can result from improper usage. Arabian Bidara is a plant that has been shown to possess antibacterial qualities, making it a viable alternative therapy. The antibacterial properties of this plant's leaves effectively combat a variety of dangerous germs and pathogens. Purposes: to determine the effectiveness of the ethanolic extract of bidara arabic leaves as an antibacterial against the growth of Escherichia coli bacteria. Methods: This research are an experimental study with a post test only control group design with the independent variable being the treatment of arabic bidara leaf extract, the dependent variable being the zone of inhibition against Escherichia coli . Positive control in the form of ciprofloxacin and negative control using distilled water. The concentrations used were 1500 ppm, 2000 ppm, 2500 ppm and 3000 ppm with three repetitions. Results: This study showed that the ethanolic extract of bidara arabic leaves could inhibit the growth of Escherichia coli bacteria with a strong zone of inhibition at concentrations of 2000 ppm, 2500 ppm, and 3000 ppm and an inhibition zone category classified as weak at a concentration of 1500 ppm. Conclusion: There is antibacterial effectiveness of the ethanolic extract of the leaves of the arabic bidara (Ziziphus spina-christi L.) against the growth of Escherichia coli bacteria. Keywords: Antibacterial; Escherichia coli ; Ziziphus spina-christi L
Effect of Ethanol Extract of Miana Leaves (Coleus atropurpureus L. Benth.) on the Growth of Pseudomonas aeruginosa and Staphylococcus epidermidis Sufiah Asri Mulyawati; Angelia Harpahda; Mario Polo Widjaya
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 12 No. 2 (2025): Juli
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

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ABSTRACT Background: Nosocomial infections continue to present a significant challenge in healthcare systems due to their high prevalence and the increasing emergence of antibiotic resistance. The bioactive constituents of miana leaves (Coleus atropurpureus L. Benth.) exhibit potential antibacterial properties, supporting the need to evaluate the effectiveness of their ethanol extract in inhibiting the growth of pathogenic microorganisms. Purpose(s): to evaluate the ability of the ethanol extract of miana leaves (Coleus atropurpureus L. Benth.) to inhibit the growth of Pseudomonas aeruginosa. Methods: A post-test only control group design was employed using miana leaf extract (Coleus atropurpureus L. Benth.) as the treatment variable, cefadroxil as the positive control, and distilled water as the negative control against Pseudomonas aeruginosa, each tested in triplicate, and the antibacterial activity was evaluated using the disc diffusion method at concentrations of 1500 ppm, 2000 ppm, 2500 ppm, and 3000 ppm. Results: The ethanol extract of miana leaves (Coleus atropurpureus L. Benth.) demonstrated strong inhibitory activity against Pseudomonas aeruginosa at concentrations of 1500–3000 ppm, and the One-Way ANOVA results showed a significant difference (p < 0.05), indicating that the extract exhibits a measurable antibacterial effect against Pseudomonas aeruginosa in vitro. Conclusion: The ethanol extract of miana leaves (Coleus atropurpureus L. Benth.) effectively inhibits the growth of Pseudomonas aeruginosa, supported by strong antibacterial activity. Keywords: antibacterial; ethanol extract; Miana leaves (Coleus atropurpureus L. Benth); Pseudomonas aeruginosa
Identification Of Bacteria Causing Nosocomial Infections In Patients In The Care Ward At Kendari City General Hospital In 2023 Sufiah Asri Mulyawati; Laode Kardin; Nina Indriyani Nasruddin; Edy Husnul Mujahid; Tsalsa Anto Ifdanunnisa
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 13 No. 1 (2025): Desember
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

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Abstract

ABSTRACT Background: Bacteria are microorganisms that have a single cell, prokaryotes. Normal skin is usually occupied by bacteria around 102-106 CFU/ml. Purpose(s): This study aims to determine the characteristics of the bacteria from hand swabs in ward patients at the Kendari City Regional General Hospital in 2023. Methods: This research is an observational descriptive study. The research sample was taken from the patients in patient room. The samples obtained were then cultured at the Microbiology Laboratory, Faculty of Medicine, Halu Oleo University. Analyzed descriptively and displayed in tabular form. Results: The results showed that there was growth of bacterial colonies. The bacteria found in the samples from the patient's hand swabs were Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus sp., Bacillus sp., Enterobacter sp. Conclusion: There was growth of bacterial colonies obtained from the results of hand swabs of ward care patients at the Kendari City Regional General Hospital.
Co-Authors Amiruddin Eso Angelia Harpahda Edy Husnul Mujahid Laode Kardin Mario Polo Widjaya Nina Indriyani Nasruddin Tsalsa Anto Ifdanunnisa Yusni Nurul Hidayah