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Keisya Nur Raudhah
STIKes Mitra Keluarga
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Effectiveness of Butterfly Pea Flower Extract (Clitoria ternatea) as a Natural Alternative Dye to Replace Methyele Blue Diffquick Coloring in Buccal Smear Cytology Preparations Keisya Nur Raudhah; Ria Amelia; Elfira Maya Sari; Danny Ernest Jonas Luhulima
Jurnal Kesehatan Vol. 14 No. 01 (2025): Jurnal Kesehatan: Volume 14, Issue 01, April 2025
Publisher : STIKES Hesti Wira Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63323/jkes.v14i01.215

Abstract

Introduction: Diffquick dying is one the methods of cytological dying. The diffquick method’s components is methylene blue. Methylene blue is toxic and non-biogradable so it requires natural dyes that do not have harmful properties for the body. Clitoria ternatea is a natural colorant that has an anthocyanin content and produces blue pigment and has been tested to color the nucleus of epithelial cells. Method: This research for the influence of temperature factors and long storage of the solution of clit extract as a nutarl colour subtitutes for methylene blue in the coloring of nucleus cells diffquick method. Research design is experimental with purposive sampling. The research was conducted in January-March 2024 at the sitohistotechnology laboratory. Results: The Mann whitney (sig.) test result 1.0>0.05 or H0 was received so there was no difference in the core color power of the cell with a 16% concentration of oak flower extract stored at 7-11⁰C dan 25⁰C as a substitute for the methylene blue dye in diffquick dyeing. Conclusion: This shows that there is no difference in the color power of the cell from the solution of the butterfly pea extract that are stored at showcase and room temperature.
Co-Authors Amelia, Ria Danny Ernest Jonas Luhulima Elfira Maya Sari