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All Journal Contagion: Scientific Periodical Journal of Public Health and Coastal Health Jurnal Kesehatan
Danny Ernest Jonas Luhulima
Universitas Kristen Indonesia
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Effectiveness of Butterfly Pea Flower Extract (Clitoria ternatea) as a Natural Alternative Dye to Replace Methyele Blue Diffquick Coloring in Buccal Smear Cytology Preparations Keisya Nur Raudhah; Ria Amelia; Elfira Maya Sari; Danny Ernest Jonas Luhulima
Jurnal Kesehatan Vol. 14 No. 01 (2025): Jurnal Kesehatan: Volume 14, Issue 01, April 2025
Publisher : STIKES Hesti Wira Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63323/jkes.v14i01.215

Abstract

Introduction: Diffquick dying is one the methods of cytological dying. The diffquick method’s components is methylene blue. Methylene blue is toxic and non-biogradable so it requires natural dyes that do not have harmful properties for the body. Clitoria ternatea is a natural colorant that has an anthocyanin content and produces blue pigment and has been tested to color the nucleus of epithelial cells. Method: This research for the influence of temperature factors and long storage of the solution of clit extract as a nutarl colour subtitutes for methylene blue in the coloring of nucleus cells diffquick method. Research design is experimental with purposive sampling. The research was conducted in January-March 2024 at the sitohistotechnology laboratory. Results: The Mann whitney (sig.) test result 1.0>0.05 or H0 was received so there was no difference in the core color power of the cell with a 16% concentration of oak flower extract stored at 7-11⁰C dan 25⁰C as a substitute for the methylene blue dye in diffquick dyeing. Conclusion: This shows that there is no difference in the color power of the cell from the solution of the butterfly pea extract that are stored at showcase and room temperature.
Reference Range of Stomatocytes in Jakarta and Surrounding Areas Danny Ernest Jonas Luhulima; Ago Harlim; Laurensius Aldrico Marveliano Sena; Ria Amelia; Erida Manalu
Contagion: Scientific Periodical Journal of Public Health and Coastal Health Vol 8, No 1 (2026): CONTAGION
Publisher : Universitas Islam Negeri Sumatera Utara, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30829/contagion.v8i1.26123

Abstract

Stomatocytes are erythrocytes characterized by a slit-like central pallor. Although they may appear in small numbers under physiological conditions, increased proportions are often associated with pathological states. Establishing population-specific reference intervals is therefore essential to avoid misinterpretation of erythrocyte morphology. This study aims to determine the reference interval for Stomatocyte counts in a healthy population from Jakarta and its surrounding areas, and to assess their relationship with routine hematological and biochemical parameters. A descriptive cross-sectional study was conducted among healthy individuals undergoing health check-ups at UKI General Hospital and East Bekasi Hospital from August 2022 to October 2024. Of 65 samples collected, 54 met the inclusion criteria. Stomatocyte counts were evaluated from peripheral blood smears by counting per 1,000 erythrocytes. Reference intervals were established using the 2.5th to 97.5th percentile method. The overall reference interval for Stomatocytes was 0 - 1.85%. Gender-specific intervals were 0 - 2.0% in men and 0 - 1.7% in women. No significant associations were observed between Stomatocyte counts and complete blood count parameters, liver function tests, or kidney function tests. In conclusion, Stomatocytes may be present at low levels in healthy individuals, with a locally derived reference interval of 0 - 1.85%. These findings highlight the importance of applying clearly defined reference intervals and considering clinical context when interpreting erythrocyte morphology to prevent overdiagnosis. Keywords: Stomatocytes, Reference Values, Erythrocyte Morphology,  Peripheral Blood Smear.
Co-Authors Ago Harlim Amelia, Ria Elfira Maya Sari Erida Manalu Keisya Nur Raudhah Laurensius Aldrico Marveliano Sena Ria Amelia