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All Journal Contagion: Scientific Periodical Journal of Public Health and Coastal Health Indonesian Journal of Global Health research Medicra (Journal of Medical Laboratory Science/Technology) Journal of Innovation Research and Knowledge Asian Journal of Community Services (AJCS) International Journal of Health and Pharmaceutical (IJHP) International Journal of Health, Engineering and Technology Jurnal Kesehatan
Danny Ernest Jonas Luhulima
Universitas Kristen Indonesia
Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Prevalence of Human Immunodeficiency Virus (HIV) Antibody Detection in Men Who Have Sex with Men (MSM): Prevalensi Deteksi Antibodi Human Immunodeficiency Virus (HIV) Pada Kelompok Lelaki Seks Lelaki (LSL) Haq, Fhasya Algina Hawatul; Amelia, Ria; Sari, Elfira Maya; Luhulima, Danny Ernest Jonas
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 7 No. 2 (2024): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v7i2.1764

Abstract

Human Immunodeficiency Virus (HIV) infection is one of the infectious infections caused by HIV that attacks the lymphocytes T tipe CD4 and can cause a decrease in the immune system. Male Sex Men (MSM) have a higher risk of HIV infection due to MSM behavior, such as having direct anal sex without using condoms or lubricant products and are more likely to change sex partners frequently. This study aims to find out the results of rapid testing of HIV antibodies on MSM in Rawalumbu and Jaka Mulya urban village. This research uses cross sectional research design, its type of research is descriptive with selection and data collection purposively sampling that carried out in February – June 2024 in Rawalumbu and Jaka Mulya urban village. Thirty-five respondents were collected. The result from a rapid test of early HIV antibodies with rapid Reagen 1 (R1) in 35 respondents, 6 respondents (17%) were reactive and 29 respondents (83%) were non-reactive. HIV antibody reactive results is 6 respondents (17%) of total 35 respondents, the most reactive outcomes in the age category 26 – 36 years.
Antenatal Care Counseling for Pregnant Women as an Effort to Prevent Stunting Simarmata, Vidi Posdo A.; Marlina, Lina; Luhulima, Danny Ernest Jonas; Udjung, Gorga I.V.W; Langi, Louisa Ariantje
Asian Journal of Community Services Vol. 3 No. 4 (2024): April 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/ajcs.v3i4.8787

Abstract

Antenatal Care aims to prepare the mother and child as well as possible, both and psychologically, as well as to protect them throughout their pregnancies, deliveries, and the postpartum period so that the condition of the mother and child at birth is physically and mentally healthy and normal. The high maternal mortality rate, including during childbirth, is a driving force for the need to provide education or counseling regarding Antenatal Care to pregnant women. With 41 pregnant women from four villages in Jatigede District, Sumedang Regency, West Java, as participants, this community service method employs an educational approach through counseling. Experts in their respective fields serve as resource people in the counseling process. The post-test results were consistent with the counseling's findings, which demonstrated a substantial increase in participants' knowledge.
Effectiveness of Butterfly Pea Flower Extract (Clitoria ternatea) as a Natural Alternative Dye to Replace Methyele Blue Diffquick Coloring in Buccal Smear Cytology Preparations Keisya Nur Raudhah; Ria Amelia; Elfira Maya Sari; Danny Ernest Jonas Luhulima
Jurnal Kesehatan Vol. 14 No. 01 (2025): Jurnal Kesehatan: Volume 14, Issue 01, April 2025
Publisher : STIKES Hesti Wira Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63323/jkes.v14i01.215

Abstract

Introduction: Diffquick dying is one the methods of cytological dying. The diffquick method’s components is methylene blue. Methylene blue is toxic and non-biogradable so it requires natural dyes that do not have harmful properties for the body. Clitoria ternatea is a natural colorant that has an anthocyanin content and produces blue pigment and has been tested to color the nucleus of epithelial cells. Method: This research for the influence of temperature factors and long storage of the solution of clit extract as a nutarl colour subtitutes for methylene blue in the coloring of nucleus cells diffquick method. Research design is experimental with purposive sampling. The research was conducted in January-March 2024 at the sitohistotechnology laboratory. Results: The Mann whitney (sig.) test result 1.0>0.05 or H0 was received so there was no difference in the core color power of the cell with a 16% concentration of oak flower extract stored at 7-11⁰C dan 25⁰C as a substitute for the methylene blue dye in diffquick dyeing. Conclusion: This shows that there is no difference in the color power of the cell from the solution of the butterfly pea extract that are stored at showcase and room temperature.
Reference Range of Stomatocytes in Jakarta and Surrounding Areas Danny Ernest Jonas Luhulima; Ago Harlim; Laurensius Aldrico Marveliano Sena; Ria Amelia; Erida Manalu
Contagion: Scientific Periodical Journal of Public Health and Coastal Health Vol 8, No 1 (2026): CONTAGION
Publisher : Universitas Islam Negeri Sumatera Utara, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30829/contagion.v8i1.26123

Abstract

Stomatocytes are erythrocytes characterized by a slit-like central pallor. Although they may appear in small numbers under physiological conditions, increased proportions are often associated with pathological states. Establishing population-specific reference intervals is therefore essential to avoid misinterpretation of erythrocyte morphology. This study aims to determine the reference interval for Stomatocyte counts in a healthy population from Jakarta and its surrounding areas, and to assess their relationship with routine hematological and biochemical parameters. A descriptive cross-sectional study was conducted among healthy individuals undergoing health check-ups at UKI General Hospital and East Bekasi Hospital from August 2022 to October 2024. Of 65 samples collected, 54 met the inclusion criteria. Stomatocyte counts were evaluated from peripheral blood smears by counting per 1,000 erythrocytes. Reference intervals were established using the 2.5th to 97.5th percentile method. The overall reference interval for Stomatocytes was 0 - 1.85%. Gender-specific intervals were 0 - 2.0% in men and 0 - 1.7% in women. No significant associations were observed between Stomatocyte counts and complete blood count parameters, liver function tests, or kidney function tests. In conclusion, Stomatocytes may be present at low levels in healthy individuals, with a locally derived reference interval of 0 - 1.85%. These findings highlight the importance of applying clearly defined reference intervals and considering clinical context when interpreting erythrocyte morphology to prevent overdiagnosis. Keywords: Stomatocytes, Reference Values, Erythrocyte Morphology,  Peripheral Blood Smear.
Ferritin in Stunting Danny Ernest Jonas Luhulima; Louise Kartika Indah
International Journal of Health and Pharmaceutical (IJHP) Vol. 6 No. 2 (2026): May 2026
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijhp.v6i2.613

Abstract

Stunting, as defined by the World Health Organization (WHO), is a form of chronic malnutrition characterized by a child’s length or height being more than two standard deviations below the median for their age group. While diagnosing stunting is relatively straightforward, the primary challenge lies in its prevention. Clinical assessment and laboratory investigations play an important role in predicting the risk of stunting. Basic laboratory evaluations include complete blood count, urinalysis, and routine stool examination. In addition, metabolic screening may involve random blood glucose, blood gas analysis, lactate, ammonia, ketone bodies, amino acid profiles, and urinary organic acids. Further investigations can include culture tests, iron profile, electrolyte levels, renal and liver function tests, thyroid hormones, infection screening, and immunoglobulin E (IgE) levels. Iron deficiency is a key contributing factor to stunting; therefore, its prevention is essential. Hemoglobin is a sensitive marker for detecting iron deficiency anemia, but it is not reliable for identifying early iron deficiency. Hemoglobin levels may remain within the normal range even when iron stores begin to decline, which can delay diagnosis if clinicians rely solely on this parameter. Serum ferritin is a more sensitive early marker, as its levels decrease in the initial stages of iron deficiency.
Normal Burr Cell Value Range in Healthy Adults Danny Ernest Jonas Luhulima; Erida Manalu; Eric Meijer; Ria Amelia
Indonesian Journal of Global Health Research Vol 7 No 4 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i4.6409

Abstract

Burr cells or also known as echinocytes are erythrocytes with short spicules evenly spaced and appear pale in the middle. Burr cells are found in patients with chronic kidney disease, burns, liver disease, uremia, microangiopathic hemolytic anemia, pyruvate kinase deficiency, post-transfusion, or can be found in the form of artifacts. This study aims to find the range of normal burr cell values in healthy adults and to find the correlation between burr cells with urea, creatinine, cholesterol, SGPT, and gender. The method used is a quantitative descriptive research design with a cross-sectional approach. Samples were taken using a purposive sampling technique, and 54 subjects who met the inclusion and exclusion criteria were analyzed for patient characteristics and laboratory results. The results showed a very weak relationship between burr cells with gender, creatinine, and SGPT with a correlation value of <0.1 and a weak relationship between burr cells with urea and cholesterol with a correlation value of >0.1 - <0.3. The normal burr cell value range was obtained with a lower limit value of 0 and an upper limit value of 30.88 burr cells per 1000 erythrocytes.
LABORATORY EXAMINATION OF HEMOLYTIC DISEASE IN NEONATES DUE TO RHESUS INCOMPATIBILITY Danny Ernest Jonas Luhulima; Christian Ronald Tanggo; Ria Amelia
Journal of Innovation Research and Knowledge Vol. 5 No. 8 (2026): Januari 2026
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hemolytic disease of the newborn (HDN) occurs when fetal red blood cells (RBCs), which possess an antigen that the mother lacks, cross the placenta into the maternal circulation, where they stimulate antibody production. The antibodies return to the fetal circulation and result in RBC destruction. Three classifications of HDN are: ABO, other causes like unexpected immune antibodies like anti-c or anti-K and Rhesus–anti D alone or may be accompanied by other Rh antibodies, examples anti-D with anti-C or anti-E. Clinical presentation of HDN varies from mild jaundice and anemia to hydrops fetalis (with ascites, pleural and pericardial effusions). This article discusses hemolytic due to incompability Rhesus anti-D.)
D-Dimer In Pregnancy Danny Ernest Jonas Luhulima; Batara Imanuel Sirait
International Journal of Health Engineering and Technology Vol. 4 No. 6 (2026): IJHET MARCH 2026
Publisher : CV. AFDIFAL MAJU BERKAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55227/ijhet.v4i6.696

Abstract

D-dimer is a fibrin degradation product that serves as a vital biomarker in the fibrinolytic system. This review aims to examine the role and mechanism of D-dimer during pregnancy and its measurement methods. The method used is a literature review of clinical studies and references related to hemostasis in pregnancy. Results show that pregnancy physiologically increases coagulation activity as a natural mechanism to prevent hemorrhage during delivery. Increased D-dimer levels are found in 27% of first-trimester pregnancies, 87% in the second trimester, and 100% in the third trimester. Pathological conditions such as preeclampsia, eclampsia, and venous thromboembolism (VTE) risks are closely linked to significant surges in D-dimer levels. While ELISA remains the gold standard for D-dimer testing, immunoturbidimetric and immunofiltration methods are more commonly used due to their speed and efficiency. In conclusion, although D-dimer levels naturally rise during pregnancy, monitoring this parameter is crucial for predicting medical emergencies like disseminated intravascular coagulation (DIC) to reduce maternal mortality rates.
Co-Authors Amelia, Ria Batara Imanuel Sirait Christian Ronald Tanggo Elfira Maya Sari Eric Meijer Erida Manalu Haq, Fhasya Algina Hawatul Harlim, Ago Indah, Louise Kartika Keisya Nur Raudhah Langi, Louisa Ariantje Laurensius Aldrico Marveliano Sena lina marlina, lina Ria Amelia Ria Amelia Simarmata, Vidi Posdo Udjung, Gorga I.V.W