Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia

Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR

Atra Romeida (Unknown)
Surjono Hadi Sutjahjo (Unknown)
Agus Purwito (Unknown)
Dewi Sukma (Unknown)
, Rustikawati (Unknown)

Article Info

Publish Date
14 May 2013

Abstract

Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid

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Journal Info
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Website

Abbrev

jurnalagronomi

Publisher

Institut Pertanian Bogor

Subject

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

Description

Aims and Scope Aims Indonesian of Journal Agronomy (Jurnal Agronomi Indonesia), the official journal of the Indonesian Society for Agronomy, publishes primary research paper, review article, policy analysis and research notes and preliminary results in all areas of agronomy. Manuscripts could be ...