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All Journal Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu-Ilmu Pertanian Indonesia Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Gema Agro
Rustikawati
Universitas Bengkulu
Agriculture, Biological Sciences & Forestry

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Cluster Analysis and SSR (Simple Sequence Repeats) Primers Selection for Aluminium Tolerance on Hot Pepper Catur Herison; Rustikawati; Ria Meita; Hasanudin
Jurnal Hortikultura Indonesia Vol. 11 No. 1 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.1.61-71

Abstract

Perakitan varietas unggul cabai merah toleran Al (Aluminium) merupakan solusi terbaik untuk mengatasi persoalan rendahnya produkvitas cabai di lahan kering masam. Identifikasi tetua toleran dan marka SSR (Simple Sequence Repeats) sangat bermanfaat dalam meningkatkan efektifitas program pemuliaan tanaman untuk merakit varietas toleran terhadap cekaman aluminium. Seleksi primer SSR adalah bagian awal yang penting dalam pengembangan marka molekuler tersebut. Penelitian bertujuan untuk menentukan kelompok genotipe toleran alumunium dan menentukan primer prospektif dalam pengembangan marka SSR untuk sifat toleran terhadap Al pada tanaman cabai. Evaluasi tingkat toleransi 27 genotipe cabai terhadap cekaman aluminium dilakukan menggunakan hidroponik sistem sumbu dengan larutan hara AB mix ditambah 500 ppm AlCl3.6H2O. Tahap selanjutnya adalah menguji 20 primer SSR dalam mengamplifikasi DNA genom cabai toleran dan peka yang mewakili. Hasil analisis cluster sifat toleransi terhadap 27 genotipe cabai menghasilkan 7 kelompok tingkat toleransi terhadap aluminium. Individu PBC396 dan PBC518 adalah genotipe yang sangat toleran terhadap cekaman aluminium yang unik sehingga keduanya sangat baik digunakan sebagai tetua dalam pengembangan cabai toleran aluminium. Primer SSR yang potensial menghasilkan marka polimorfik untuk sifat toleran terhadap cekaman aluminium pada cabai adalah TMS7 dan CAMS358. Primer tersebut selanjutnya harus diuji pada mapping population untuk menentukan pita yang dapat digunakan sebagai penanda toleransi terhadap aluminium. Kata kunci: Capsicum annuum, NTSYS, sidik gerombol, tenggang masam
KERAGAAN PERTUMBUHAN VEGETATIF DAN REPRODUKTIF HIBRIDA JAGUNG PERSILANGAN GALUR INBRIDA MUTAN (M4) PADA LATOSOL DARMAGA Rustikawati Rustikawati; Catur Herison; Surjono H. Sutjahjo
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 12, No 1 (2010)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.12.1.55-60

Abstract

[VEGETATIVE AND REPRODUCTIVE PERFORMANCES OF MAIZE HYBRIDS GENERATED FROM INBRED MUTANTS (M4) ON DARMAGA LATOSOL]. The development of hybrids from local germplasms are extensively done as alternative to the expensive imported hybrid seeds. The farthest genetic distance of parental lines is required to develop the best hybrids. The objective of this study was to compare the vegetative and reproductive performances of hybrids generated from crosses of M4 inbred lines, generated from the selected gamma irradiated mutants. Twenty eight hybrids derived from diallel crosses of M4 inbred lines and a check hybrid variety (NK33) were evaluated on Darmaga latosol. The results showed that all hybrids had lower performances on vegetative and reproductive characters compared to NK33. However, based on the selection index involving all characters, hybrids of G3 x G8, G6 x G8, and G8 x G6  with the index values of   9.45, 7.85, and 6.72, respectively,  performed better than or similar to  NK33.
APLIKASI TIGA JENIS PUPUK ANORGANIK TERHADAP PERTUMBUHAN DAN HASIL TIGA VARIETAS CABAI HIBRIDA UNIB (Capsicuum annuum L.) Andi Tri Maryono; Dwi Wahyuni Ganefianti; Bambang Gonggo Murcitro; Rustikawati Rustikawati; Herry Gusmara; Mukhtasar Mukhtasar; Umi Salamah
AGRITROP Vol 17, No 2 (2019): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v17i2.2396

Abstract

Penggunakan benih lokal yang ditanam secara terus menerus telah menyebabkan produksi rendah. Upaya untuk meningkatkan produktivitas cabai yaitu dengan menggunakan benih hibrida unggul yang memiliki produktivitas yang tinggi, umur panen genjah, memiliki daya tahan terhadap hama dan penyakit, dan buah yang disukai para konsumen serta daya adaptasi lingkungan yang tinggi. Rancangan Percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial, yang terdiri atas 2 faktor.  Faktor pertama adalah jenis pupuk terdiri atas 3 jenis yaitu : NPK Mutiara (16:16:16) = 300 kg/ha, Urea+KCl+TSP = 250 kg/ha+500 kg/ha+400 kg/ha, ½ dosis Urea+½ dosis KCl+½ dosis TSP+½ dosis NPK Mutiara (16:16:16) = 125 kg/ha+250 kg/ha+200 kg/ha+150 kg/ha. Faktor kedua adalah Varietas cabai hibrida terdiri atas 3 varietas yaitu : UNIB C H73, UNIB C H65 dan, UNIB C H13. Dari kedua faktor tersebut diperoleh 9 pasang perlakuan, diulang 5 kali sehingga diperoleh 45 satuan percobaan, yang terdiri atas 2 tanaman sehingga didapatkan 90 tanaman.Hasil penelitian menunjukkan bahwa aplikasi pupuk anorganik urea+KCl+TSP menghasilkan jumlah buah total tertinggi pada varietas UNIB C H65. Aplikasi pupuk anorganik Urea+KCl+TSP menghasilkan tinggi tanaman yang lebih tinggi dari aplikasi pupuk anorganik lainnya, dengan umur berbunga yang lebih cepat dibandingakan aplikasi pupuk anorganik lainnya. Aplikasi pupuk anorganik pada varietas UNIB C H73 menghasilkan diameter batang lebih besar, umur panen yang relatif cepat, panjang tangkai buah lebih panjang, panjang buah terpanjang dan bobot buah segar terberat dibandingkan varietas lainnya.
Respon Temu Putih dan Temu Mangga dengan Pemberian BA dan 2,4-D secara In Vitro Muhammad Isra’ Aulia; Rustikawati; Entang Inoriah
GEMA AGRO Vol 25 No 2 (2020)
Publisher : Fakultas Pertanian, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.789 KB) | DOI: 10.22225/.25.2.2608.92-102

Abstract

Calus and shoot induction of curcuma sp requires the balance amount of auxin and cytokinine which are suitable for the plant species. This study was aimed at evaluating the response of ‘temu putih’ (Curcuma zedoaria Rosc.) and ‘temu mangga’ (Curcuma mangga Val.) to the supplementation of 5 ppm BA and several levels of 2,4-D on the MS medium of in vitro culture. The study was conducted in the Laboratory of Agronomy, Department of Agriculture, Faculty of Agriculture, the University of Bengkulu, from December 2018 to February 2019. The plant material was explans isolated from the buds of curcuma tubers. The experiment was conducted in a completely randomized design arranged in factorial with 2 factors. The first factor was the kinds of curcuma i.e . C. zedoaria and C.mangga. The second factor was the concentration of 2,4–D i.e. 0 ppm, 1 ppm and 2 ppm. The base culture medium was MS with 5 ppm BA. The experimental unit consisted of three botle each of which containing of 1 explant. The treatment was repeated three times amounting to 54 experimental units. The results indicated that both curcuma had different growth respon to in vitro culture. Addition of 2,4-D to the MS medium containing 5 ppm BA produced cally. Callus induction was formed in in vitro culture of Curcuma zeodiaria suplemented with 1 ppm 2,4-D. Up to 2 ppm of 2,4-D, cally production of Curcuma mangga still increasing. Addition of 5 ppm of BA 5 to MS medium induced shoot formation in Curcuma zeodiaria but not in Curcuma mangga.
KORELASI DAN SIDIK LINTAS KARAKTER PERTUMBUHAN DAN KOMPONEN HASIL DENGAN HASIL PADA 15 GENOTIPE HIBRIDA JAGUNG Hellianti Pennita; Catur Herison; Marwanto Marwanto; Rustikawati Rustikawati
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 22 No 1 (2020)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.22.1.1-8

Abstract

[PATH ANALYSIS OF GROWTH CHARACTERS AND YIELD COMPONENTS WITH THE YIELD OF 15 GENOTYPES OF MAIZE HYBRID].  Determination of traits used as the selection criteria are based on the trait(s) that have a close relationship to the yield.  The aims of this research were to measure the closeness of the relationship between morphological (growth and yield component) traits and crop yield, and to determine the trait that directly or indirectly affected on maize yield.  The research was conducted in Rimbo Kedui’s Village, South Seluma Subdistrict, Seluma District, Bengkulu Province on May 2017 to August 2017.  The experimental design used was a Complete Randomized Block Design (CRBD) in a single factor with 3 replications.  The treatments were 15 maize hybrid genotypes, consisting of CT1, CT2, CT3, CT4, CT5, CT6, CT7, CT8, CT9, CT10, CT11, CT12, CT13, CT14, and CT15.  The data were statistically analyzed by analysis of varians (ANOVA) with P(a=5%).  The qualitative data were analyzed descriptively. The result of correlation analysis revealed that the yield of maize hybrids highly correlated to some growth and yield component factors.  High-yielding maize hybrids were supported by higher ear diameter, plant fresh weight, plant dry weight, plant height, the number of seeds per ear.  Plant dry weight became the variable that has the highest direct effect with positive correlation on the yield.  The second highest rank was the number of seeds per ear with also a positive correlation value.  Yield improvement of maize hybrid, therefore, can be obtained by fostering the plant total biomass and/or by multiplying the number of seeds per ear.
KERAGAAN PERTUMBUHAN DAN HASIL 20 GENOTIPE CABAI MERAH (Capsicum annuum L.) DI DATARAN RENDAH Viona Juanda Putri; Catur Herison; Merakati Handajaningsih; Marulak Simarmata; Rustikawati; Sukisno
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 27 No 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.27.1.40-46

Abstract

[GROWTH ADAPTATION AND PRODUCTIVITY OF 20 CAYENNE PEPPERS (Capsicum annuum L.) GENOTYPES IN LOWLANDS AREA]. The cultivation of chili peppers in lowland areas of Indonesia faces several environmental challenges. Efforts to increase red chili production in lowland regions include planting genotypes that are adaptive to local environmental conditions. This study aims to obtain information on the best red chili genotypes that can adapt and produce well in lowland areas. The research was conducted from May 2024 to September 2024 using the Completely Randomized Block Design (CRBD) method with a single-factor treatment, consisting of 20 genotypes red chili with three replications. The data collected included growth variables (plant height, first dichotomous height, leaf area, number of dichotomous points, stem diameter, and plant dry biomass) and yield variables (first harvest age, number of fruits per plant, fruit diameter, fruit length, fruit weight per plant, and percentage of marketable fruit). The results showed that the ‘Lotanbar’ genotype exhibited the best performance during the vegetative phase, as indicated by the largest total leaf area, the highest number of dichotomous points, the largest stem diameter, and the highest plant dry weight compared to other genotypes. Meanwhile, the ‘Baja F1’ genotype demonstrated superior performance during the generative phase, producing the largest fruit diameter and the highest fruit weight per plant, reaching up to 200% higher than the genotype with the lowest yield. Thus, the ‘Lotanbar’ genotype is recommended for optimization during the vegetative growth phase, while ‘Baja F1’ is more ideal for increasing yield during the generative phase in red chili cultivation in lowland areas.  
Co-Authors Andi Tri Maryono Bambang Gonggo Murcitro Dwi Wahyuni Ganefianti Entang Inoriah Hasanudin Hellianti Pennita Herison, Catur Herry Gusmara Marulak Simarmata Marwanto Marwanto Merakati Handajaningsih Muhammad Isra’ Aulia Mukhtasar Mukhtasar Ria Meita Sukisno Surjono H. Sutjahjo Umi Salamah Viona Juanda Putri