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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu-Ilmu Pertanian Indonesia AGRIVITA, Journal of Agricultural Science Agrin : Jurnal Penelitian Pertanian TERRA : Journal of Land Restoration AGRITROPICA : Journal of Agricultural Sciences Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Gema Agro Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Community Development Journal: Jurnal Pengabdian Masyarakat JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Pada Masyarakat Jurnal Imiah Pengabdian Pada Masyarakat (JIPM)
Rustikawati
Universitas Bengkulu
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EVALUATION OF GROWTH AND YIELD PERFORMANCE ON INOCULATED CHILI PEPPER HYBRIDS BY CUCUMBER MOSAIC VIRUS Herison, Catur; Handayaningsih, Merakati; Fahrurrozi, Fahrurrozi; Rustikawati, Rustikawati
AGRIVITA, Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Tolerance cultivar is a best control measure to reduce losses due to virus attack in the field.  Study on growth and yield of new cultivars compared to the commercial ones is an important step in cultivar development whether they will be accepted by farmers or not. The objective of this research was to study growth and yield of new chili pepper hybrids developed for CMV tolerance in comparison to a commercial and similar fruit size one. Twelve new hybrids and one commercial hybrid cultivar Prada, as a control, were evaluated in a completely randomized design with 3 replications. In the greenhouse experiment, H23 and H20 showed better vegetative growth compared to other hybrids and control. Hybrid H17 showed the highest yield followed by H5, H4 and H6.  Keywords.  growth, yield, chili pepper hybrids, CMV tolerance
DNA MARKER-ASSISTED AND MORPHOLOGICAL SELECTION ON BC3 GENOTYPES SHORTCUT THE INTROGRESSION OF CMV TOLERANCE GENES ON CHILI PEPPER Herison, Catur; Winarsih, Sri; Handayaningsih, Merakati; Rustikawati, Rustikawati
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Superior hybrid and CMV tolerant cultivar development requires a pair of high heterobeltiosis parents and both of which are CMV tolerant.  Gene introgression has to be accomplished if the tolerance does not exist in the parents. This research was objected to employ DNA markers and morphological traits on identifying the most resemble BC3 CMV tolerant individuals to their recurrent parents to shortcut the backcrossing cycle. This research used fifteen BC3 populations generated from crosses of hybrid parents PBC1354 and PBC378 with tolerant parents of C1024, C1042 and C1043.  The BC3 populations were previously selected for their CMV tolerance and were characterized by RAPD technique and morphological traits. Selection by means of both RAPD marker and morphological traits identified BC3 individuals 99.9% resemble to their recurrent parent. Genotypes B3A24-20, B3A29-13, B3A29-22, B3B12-13, B3B37-9, B3B12-25, B3C16-5, B3C16-16, B3C34-18 99.9% were similar to PBC378, and  B3D11-8, B3D11-17, B3D38-5, B3E12-17, B3E20-22, B3E31-19  were 99.9% similar to PBC1354.   Those genotypes were both similar to their recurrent parent and tolerance to CMV. The employed strategy shortcut CMV tolerance genes introgression through a backcross breeding.
IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.102

Abstract

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.  On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.  Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.   The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.  The initial step of the research was to select random primers could positively amplify the maize DNA.  The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.  Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.  Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.
The Effects of Gamma Irradiation on Growth Response of Rodent Tuber (Typhonium flagelliforme Lodd.) Mutant in In Vitro Culture NESTI FRONIKA SIANIPAR; ARIANDANA WANTHO; . RUSTIKAWATI; WILMAR MAARISIT
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.697 KB) | DOI: 10.4308/hjb.20.2.51

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is an Indonesian native plant that potential as anticancer. Even though the potential use of bioactive compounds from T. flagelliforme is very high, its genetic variation in Indonesia is low. Gamma irradiation can be used to increase genetic variation. The objective of this research was to obtain the first generation mutants (M1) of T. flagelliforme resulting from gamma irradiation of normal mother plantlets with different plant height and number of shoots. T. flagelliforme was irradiated at the doses of 0, 10, 20, 30, 40, and 50 Gy. The percentage of plant death was then calculated to determine the Lethal Dose (LD) 50. T. flagelliforme was then re-irradiated at LD50 dose to obtain M1 mutant. The plant height and number of shoots variation was observed after eight weeks of culture. The analysis of T. flagelliforme radiosensitivity resulted in LD50 at 25 Gy. Observation at six weeks after irradiation showed significant differences between the normal mother plant, 20 Gy mutants, and 25 Gy mutants with an average plant height of 9.57, 3.41, and 2.43 cm respectively, and the  average number of shoots produced was 7.85, 6.03, and 5.00 shoots respectively. Irradiation at 20 and 25 Gy produced 49 and 37 mutant plantlets. This research showed that gamma irradiation at LD50 dose could generate plant height and number of shoots variation of M1 mutant plantlets that are different from normal mother plantlets.
Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Indonesian Journal of Agronomy Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.864 KB) | DOI: 10.24831/jai.v40i3.6829

Abstract

Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid
Optimasi Pertumbuhan dan Multiplikasi Lini Klon PLBs Anggrek Spathoglottis plicata Blume melalui Modifikasi Komposisi Medium MS dan Sitokinin Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Jurnal Hortikultura Indonesia Vol. 4 No. 1 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.907 KB) | DOI: 10.29244/jhi.4.1.1-8

Abstract

ABSTRACTIn order to enhance the multiplication of  Protocorm Like Bodies (PLBs)  of Spathoglottis  plicataorchid  in vitro, several combinations of vitamin, sucrose concentration, and cytokinin were tested.   Thisexperiment was arranged in Completely Randomized Design (CRD) with two factors. The first factor was combination  of  vitamin  composition  and  sugar  concentrations  in  the medium  which  consisted  of  four different  combinations  (J1 = vitamin  MS + sucrose 30 g  L-1, J2 = vitamin B5 + sucrose 30 g  L-1, J3= vitamin  MS  + sucrose  40  g  L-1,  J4  =  vitamin  B5  +  sucrose  40  g  L-1).  The  second factor  was  seven combinations  of  cytokinin types and its  concentrations (S0 = without cytokinin (control), S1 =    20  μM BA, S2 = 40  μM BA, S3 = 20 μM kinetin,   S4 = 40  μM  kinetin, S5 = 75  ml L-1 coconut milk, and S6 = 150 ml L-1 coconut milk).  PLBs  were used as  explant  and were  grown on MS  solid medium containing various vitamin, sucrose, and cytokinin combinations  as mentioned above.  Growth and multiplication of PLBs were based on  the number of PLBs per explant, number of plantle ts per explant, number of roots formed, plantlet height and visual appearance and performa nce of the observations at 6  MST.  The best growth  and  multiplication  of  PLBs  orchid S.  plicata  was  produced  on  MS  medium  modified  with  B5 vitamins  and 30 g  L-1 sucrose, followed by those and  on MS medium enriched  with  75 ml  L-1 coconut milk and on MS medium supported by 20 μM BA.Key words : B5, benzyl adenine, coconut water, in vitro , orchid, sugar concentration,  vitamin compositionABSTRAKPercobaan ini bertujuan untuk mendapatkan formulasi kom posisi vitamin dan konsentrasi gula medium,serta jenis dan  konsentrasi sitokinin terbaik dalam menginduksi pertumbuhan dan  multiplikasi  lini klon PLBsanggrek Spathoglottis plicata dalam jumlah yang besar  secara  in vitro. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) Faktorial.  Eksplan yang digunakan adalah PLBs  yang ditanam pada  medium Murashige dan  Skoog  (MS)  yang  telah   dilakukan  modifikasi  sesuai  dengan  perlakuan.  Faktor  pertama  adalah  formulasi komposisi vitamin dan konsentrasi gula yang terdiri dari empat macam formulasi yaitu J1 = vitamin  MS + gula 30 g L-1, J2 = vitamin B5 + gula 30 g  L-1, J3= vitamin MS + gula 40 g L-1, J4 = vitamin B5 + gula 40 g  L-1. Faktor kedua adalah penambahan sitokinin (3 jenis dengan 2 taraf konsentrasi) yang terdiri dari 7 kombinasi perlakuan yaitu S0 = tanpa sitokinin (kontrol), S1 = BA 20 μM, S2 = BA 40 μM, S3 = kinetin 20 μM,  S4 = kinetin 40 μM, S5 = air kelapa 75 ml L- 1, dan S6 = air kelapa 150 ml  L-1. Pertumbuhan dan multiplikasi PLBs anggrek S. plicataterbaik  dihasilkan   pada medium  MS  dengan  modifikasi  vitamin  B5  dan  konsentrasi  gula  30  g  L-1dan  pada medium MS dengan penambahan air kelapa 75 ml L- 1 serta pada medium MS dengan penambahan BA 20  μMdengan  kriteria  jumlah  PLBs akhir dan  jumlah  planlet  akhir  tertinggi,  jumlah  akar  dan  tinggi  tanaman serta penampilan visual hasil pengamatan pada 6 minggu setelah tanam.Kata kunci : air kelapa, anggrek, B5, benzyl adenin, in vitro, komposisi vitamin, konsentrasi gula
Cluster Analysis and SSR (Simple Sequence Repeats) Primers Selection for Aluminium Tolerance on Hot Pepper Catur Herison; Rustikawati; Ria Meita; Hasanudin
Jurnal Hortikultura Indonesia Vol. 11 No. 1 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.1.61-71

Abstract

Perakitan varietas unggul cabai merah toleran Al (Aluminium) merupakan solusi terbaik untuk mengatasi persoalan rendahnya produkvitas cabai di lahan kering masam. Identifikasi tetua toleran dan marka SSR (Simple Sequence Repeats) sangat bermanfaat dalam meningkatkan efektifitas program pemuliaan tanaman untuk merakit varietas toleran terhadap cekaman aluminium. Seleksi primer SSR adalah bagian awal yang penting dalam pengembangan marka molekuler tersebut. Penelitian bertujuan untuk menentukan kelompok genotipe toleran alumunium dan menentukan primer prospektif dalam pengembangan marka SSR untuk sifat toleran terhadap Al pada tanaman cabai. Evaluasi tingkat toleransi 27 genotipe cabai terhadap cekaman aluminium dilakukan menggunakan hidroponik sistem sumbu dengan larutan hara AB mix ditambah 500 ppm AlCl3.6H2O. Tahap selanjutnya adalah menguji 20 primer SSR dalam mengamplifikasi DNA genom cabai toleran dan peka yang mewakili. Hasil analisis cluster sifat toleransi terhadap 27 genotipe cabai menghasilkan 7 kelompok tingkat toleransi terhadap aluminium. Individu PBC396 dan PBC518 adalah genotipe yang sangat toleran terhadap cekaman aluminium yang unik sehingga keduanya sangat baik digunakan sebagai tetua dalam pengembangan cabai toleran aluminium. Primer SSR yang potensial menghasilkan marka polimorfik untuk sifat toleran terhadap cekaman aluminium pada cabai adalah TMS7 dan CAMS358. Primer tersebut selanjutnya harus diuji pada mapping population untuk menentukan pita yang dapat digunakan sebagai penanda toleransi terhadap aluminium. Kata kunci: Capsicum annuum, NTSYS, sidik gerombol, tenggang masam
KERAGAAN PERTUMBUHAN VEGETATIF DAN REPRODUKTIF HIBRIDA JAGUNG PERSILANGAN GALUR INBRIDA MUTAN (M4) PADA LATOSOL DARMAGA Rustikawati Rustikawati; Catur Herison; Surjono H. Sutjahjo
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 12, No 1 (2010)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.12.1.55-60

Abstract

[VEGETATIVE AND REPRODUCTIVE PERFORMANCES OF MAIZE HYBRIDS GENERATED FROM INBRED MUTANTS (M4) ON DARMAGA LATOSOL]. The development of hybrids from local germplasms are extensively done as alternative to the expensive imported hybrid seeds. The farthest genetic distance of parental lines is required to develop the best hybrids. The objective of this study was to compare the vegetative and reproductive performances of hybrids generated from crosses of M4 inbred lines, generated from the selected gamma irradiated mutants. Twenty eight hybrids derived from diallel crosses of M4 inbred lines and a check hybrid variety (NK33) were evaluated on Darmaga latosol. The results showed that all hybrids had lower performances on vegetative and reproductive characters compared to NK33. However, based on the selection index involving all characters, hybrids of G3 x G8, G6 x G8, and G8 x G6  with the index values of   9.45, 7.85, and 6.72, respectively,  performed better than or similar to  NK33.
Genetic Diversity Analysis in 27 Tomato Accessions Using Morphological and Molecular Markers Catur Herison; Surjono H. Sutjahjo; Ineu Sulastrini; Rustikawati Rustikawati; Siti Marwiyah
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.726

Abstract

Genetic diversity is the most important aspect in tomato breeding activities. Better assessment on the diversity of the collected accessions will come up with better result of the cultivar development. This study aimed at analyzing the genetic diversity of 27 tomato accessions by morphological and molecular markers. Twenty seven accessions collected from various regions of Indonesia were planted in the field and evaluated for their morphological traits, and RAPD analyzed for their molecular markers. The UPGMA clustering analyzes, elaborating the combination of morphological and molecular data, indicated that the tomato accessions could be grouped into 5 major groups with 70 % genetic similarity levels. Current study indicated that although many accessions came from different locations, they congregated into the same group. Cherry, Kudamati 1 and Lombok 3 were the farthest genetic distant accessions to the others. Those three genotypes will be the most valuable accessions, when they were crossed with other accessions, for designing a prospective breeding program in the future.
Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas Rustikawati Rustikawati; Catur Herison; Entang Inoriah; Vera Dwisari
AGRITROPICA : Journal of Agricultural Sciences Vol 4, No 1 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.4.1.82-92

Abstract

Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation.  The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design.  The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm.  The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.).  Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud.  Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color.  Mean comparison was performed by the Least Significant Difference (LSD).  The results showed that there was no interaction between BAP concentration and genotype on any variable observed.  The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro.  The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.
Co-Authors A.W. Sandhi S.G. Adeputri, Eriana Agus Purwito Almunawar, Said Agil Alnopri Alnopri Alnopri, Alnopri Amanda, Kiki Rizki Andi Tri Maryono Angraini, Dila ARIANDANA WANTHO Atra Romeida Atra Romeida Bambang Gonggo Murcitro Bertham, Yudhy H Bilal Chairul Ahmad Deo Surya Anjaya Dewi Sukma Dival Satria Gunawan Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwitama, Niko Edhi Turmudi Edi Susilo Eko Suprijono Ela Hasri Windari, Ela Hasri Windari Emilda Tri Mauli Entang Inoriah Entang Inoriah Fahrurrozi, Fahrurrozi Fernandez, Regi Hasanudin Hellianti Pennita Herison, Catur Herry Gusmara Hindarto, Kanang Setyo Indres, Indres Ineu Sulastrini Inoriyah, Entang Joko Suharjo, Usman Kris Kamil, Maulana Insanul Laksono Trisnantoro Luwih, Bagus Edi Maemunah Maemunah Marulak Simarmata Marulak Simarmata Marulak Simarmata Marwanto Marwanto Meko Gustian Merakati Handajaningsih Merakati Handajaningsih Merakati Handajaningsih Merakati Handayaningsih Merakati Handayaningsih Mimi Sutrawati Muhammad Chozin, Muhammad Muhammad Isra’ Aulia Muhammad Ravie Mukhtasar Mukhtasar Mukhtasar, Mukhtasar Nabila, Athya Kania Nadia, Nur Ain NESTI FRONIKA SIANIPAR Nurjanah, Uswatun Pardosi, Santi K. Parwito Parwito Reny Herawati Ria Meita S. H. Sutjahjo Saputra, Pringky Okta Saragih, Rosa Sri Andari Sianipar, Mangara W Sri Winarsih Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Sudarsono Suharjo, Usman Kris Joko Sukisno Sukisno Sukisno Sukisno, Sukisno Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono Hadi Sutjahjo Surmaini, Ervi Suryati, Dotti Syafira Dwi Wulandari Syafitri, Alya Novia Syakia Kurin, Aricha Umi Salamah Vera Dwisari Viona Juanda Putri Widodo Widodo Wilmar Maarisit Windari, Ela Hasri Wuri Prameswari Yulian, Yulian