Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study

Sijani Prahastuti (Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia)
Meilinah Hidayat (Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia)
Stella Tinia Hasiana (Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia)
Wahyu Widowati (Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40163, West Java, Indonesia)
Annisa Amalia (Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia)
Rismawati Laila Qodariah (Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia)
Rizal Rizal (Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia)
Satrio Haryo Benowo Wibowo (Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia)
Hanna Sari Widya Kusuma (Aretha Medika Utama, Biomolecular Research Center, Jalan Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia)

Majalah Obat Tradisional
Vol 24, No 3 (2019)

Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of   FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control,  EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model.