Lactobacillus plantarum IS-20506 Probiotic Restores Galectin-4 and Myosin-1a Expressions in Duodenum, Jejunum and Ileum of Lipopolysaccharide-induced Rats

Reza Gunadi Ranuh (Department of Child Health, Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 6-8, Surabaya)
Alpha Fardah Athiyyah (Department of Child Health, Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 6-8, Surabaya)
Andy Darma (Department of Child Health, Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 6-8, Surabaya)
Wibi Riawan (Laboratory of Biochemistry and Biomolecular, Universitas Brawijaya, Jl. Veteran, Malang)
Ingrid Suryanti Surono (Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta 11480)
Ferry Sandra (Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No.260, Jakarta)
Subijanto Marto Sudarmo (Department of Child Health, Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Jl. Prof. Dr. Moestopo No. 6-8, Surabaya)

The Indonesian Biomedical Journal
Vol 12, No 3 (2020)

BACKGROUND: Galectin-4 and Myosin-1a are important proteins for normal intestinal brush border structure and composition. Damage of these proteins by inflammation may alter digestion, absorption and barrier function. Probiotic has been widely known in maintaining gut health. However, the molecular mechanism of Lactobacillus plantarum IS-2056 probiotic in repairing intestinal brush border is not well defined. Therefore, current study was conducted by investigating the Galectin-4 and Myosin-1a expressions in a rodent model.METHODS: Male Wistar rats were induced with/without lipopolysaccharide (LPS) and treated with/without L. plantarum IS-2056 probiotic. On the seventh day, duodenum, jejunum, and ileum were collected and analyzed with western blot and immunohistochemistry for Galectin-4 and Myosin-1a expressions.RESULTS: Rats administrated with L. plantarum IS-2056 probiotic showed significant increase of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum compared to the control group (p<0.05). While in control group, Galectin-4 level tended to increase in more distal of intestinal segment and Myosin-1a level tended to decrease in more distal intestinal segment.CONCLUSION: L. plantarum IS-20506 probiotic may facilitate the repairment of damaged intestinal brush border as demonstrated by significant restoration of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum of LPS-induced rats.KEYWORDS: Lactobacillus plantarum, IS-20506, probiotic, galectin-4, myosin-1a, duodenum, jejunum, ileum