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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung The Journal of Experimental Life Sciences (JELS) Indonesian Journal of Cancer Chemoprevention Program Kreativitas Mahasiswa - Penelitian The Indonesian Biomedical Journal Majalah Kesehatan FKUB KESANS : International Journal of Health and Science Jurnal Ilmiah Kesehatan (JIKA)
Wibi Riawan
Biochemistry And Biomolecular Laboratory, Faculty Of Medicine, Universitas Brawijaya
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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YLS-THERAPY: POTENSI SUPERNATAN YOGURT LBA–ST SEBAGAI ANTIKANKER SERVIKS BERBASIS BIOMOLEKULER Permata Sari, Desie Suci; Ridho, Wildan Mochamad; Lizziyyannida, Lizziyyannida; Sholihah, Zurniatus; Putra Pratama, Satya Hanggara; Rahmani, Erita; Riawan, Wibi
Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.974 KB)

Abstract

Cervical cancer is the third most diagnosed cancer and the fourth leading cause of cancer death in females in the world. Zebrafish have been used as experimental animals in human tumors because of similar gene expression (70%) than other experimental animals. Yogurt LBA-ST produces SCFA metabolites (acetate, lactate, and butyrate) that can provide anticarcinogenic effects. There has been a vast growth of related research focusing on proliferation and apoptosis without clear mechanism. However, Important concern need to considered in the development of tumors is protein-coding invasion, migration, and adhesion (IMA) of tumor cells. This study aims at investigating (1) Antiproliferation activities of supernatan yogurt LBA-ST (SY LBA-ST) on zebrafish embryos and HeLa cells, and (2) anti-IMA’s pathway on HeLa cells. The anti-cancer property of SY LBA-ST was evaluated by MTT assay for viability and imunositochemistry of MMP-2, Laminin5-y2, Hsp27, and TGF-β for anti-IMA mechanism. The data is collected and analyzed using One-Way ANOVA.This study could be concluded that (1) SY LBA-ST increase mortality rate and decreased the hatching rate in zebrafish embryos, LC50 ≈ 20% (v/v),  (2) supernatant, pellet, and whole yogurt shows antiproliferation activity on HeLa cells, (3) IC50 SY LBA-ST ≈ 30% (v/v) and the ED = 10% (v/v), and (4) the anti-IMA’s pathway was via decrease MMP-2, Laminin5-y2, Hsp27, and TGF-β expression (p<0.05 ± 0.005).Keywords:  lactobacillus bulgaricus-acidophilus, streptococcus thermophillus, cancer, HeLa, zebrafish
PENINGKATAN EKSPRESI LAMININ NAMUN TIDAK VE-CADHERIN PADA SAWAR DARAH OTAK SETELAH INFEKSI Mycobacterium tuberculosis INTRAPULMONALIS Widayati, Aris; Wulandari, Laksmi; Riawan, Wibi
Majalah Kesehatan FKUB Vol 5, No 3 (2018): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.512 KB) | DOI: 10.21776/ub.majalahkesehatan.005.03.3

Abstract

Tuberkulosis merupakan  masalah kesehatan utama di dunia. Pada tahun 2016, WHO menemukan angka kejadian TB kurang lebih 10,4 juta, dan untuk Indonesia dilaporkan sebesar 156.723 kasus. Meskipun penyebaran Mycobacterium tuberculosis di susunan saraf pusat tercatat hanya 1%, namun memiliki tingkat kecacatan dan kematian yang tinggi, sehingga menuntut adanya tatalaksana yang efektif untuk mengatasinya. VE-chaderin dan laminin merupakan protein adhesin yang berfungsi mengendalikan permeabilitas pembuluh darah dan mempertahankan integritas blood brain barrier, sehingga kedua protein adhesin tersebut dapat menjadi salah satu target terapi tuberkulosis otak. Penelitian ini bertujuan untuk mengetahui efek paparan M. tuberculosis secara inhalasi terhadap ekspresi laminin dan VE-chaderin pada sel endotel blood brain barrier. Penelitian ini menggunakan mencit Balb/c  (Mus musculus) yang diinfeksi oleh M.tuberculosis strain H37Rv secara inhalasi. Jaringan otak diperiksa menggunakan metode imunohistokimia dengan  antibodi mt-38, antibodi VE-chaderin dan laminin. Hasil penelitian menunjukkan adanya invasi M. tuberculosis pada  mikroglia jaringan otak mencit, diiketahui juga adanya peningkatan ekspresi laminin, sedangkan VE-chaderin tidak menunjukkan adanya perubahan. Proses masuknya M. tuberculosis ke otak diduga terjadi melalui proses diapedesis atau melalui peningkatan ekspresi laminin tanpa perubahan pada VE-chaderin dan reseptor laminin diduga sebagai tempat berikatan yang memungkinkan bakteri tersebut masuk ke jaringan otak. 
ANALISIS LOSS OF HETEROZYGOSITYGEN RAR β2 PADA PATOGENESIS KANKER PAYUDARA Muliartha, I Ketut Gede; Riawan, Wibi; Hidayat, Asnah; Armania, Fitri
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.671 KB) | DOI: 10.21776/ub.jkb.2008.024.02.5

Abstract

The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (RAR β2) gene on the chromosom 3p24 in the pathogenesis ofbreast cancer. The breast cancer is the most malignant tumor that leads the second cause of death among the female. The recent concept of the theory of pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressorgene, and growth promoting factor gene. However, the diagnostic and therapy of breast cancer are still not satisfied. The lost or decreased in the expressionof RAR  β2gene on 3p24 chromosome via loss of heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is by experimental and observational. Human female breastcancer and blood samples were gained from public hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident in 3p24 chromosome was detected by PCR and Chromogenic in situ Hybridization (CISH) method.
CYCLOOXYGENASE-2 (COX-2) EXPRESSION ON TESTIS CONNECTIVE TISSUE OF Rattus norvegicusAFTER TREATMENT WITH BETEL NUT EXTRACT (Areca catechu) Akmal, Muslim; Riawan, Wibi
Jurnal Kedokteran Brawijaya Vol 23, No 3 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.806 KB) | DOI: 10.21776/ub.jkb.2007.023.03.3

Abstract

Betel nut contains alcaloids such as arecoline, arecaine, arecaidine, arecolidine, guvacine, guvacoline and isoguvasine. Arecoline has ability to change gonad morph-function, including shape abnormality of sperm. This research was conducted to prove the ability of betel nut extract (Areca catechu) in causing apoptosis on testis connective tissue of Rattus novergicus. This research used male; 2-3 months age, 150-200 grams body weight of white rats Rattus norvegicus strain Wistar. The rats were divided into 5 groups in equalnumber, 3 rats respectively. They were a control group without treatment and 4 groups as treatment groups which were given doses of betel nut extract, i,e., 1, 2, 3  and 4 gram during seven days. The result showed that dose variation ofbetel nut extract could induction of COX-2 expression on rats (Rattus norvegicus) strain Wistar testicular seminiferous tubule. Keywords: Betel nut extract, Rattus norvegicus, testis, cyclooxygenase-2
HUBUNGAN ANTARA DERAJAT OBSTRUKSI SERANGAN AKUT ASMA DENGAN KADAR IgE, IFN- DAN IL-4 TOTAL SERUM Kusuma, HMS. Chandra; Kalim, Kusworini Handono; Riawan, Wibi; Muid, Masdar
Jurnal Kedokteran Brawijaya Vol 20, No 2 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.883 KB) | DOI: 10.21776/ub.jkb.2004.020.02.4

Abstract

ABSTRACT Acute bronchial asthma is a common medical emergency the world over. There now exists compelling evidence of a role for Th2-Th1 paradigm in the pathogenesis of asthma in children. We hypothesized that childhood asthma is associated with the activation of  Th2-Th1 lymphocytes whose products regulate at least in part, the expression of IgE, IFN-γ and IL-4 and thereby disease severity. Our aims, therefore were to compare the level of total IgE, total IL-4 and total IFN-γ in serum from asthmatic and non asthmatic control children matched for age and sex, and to attempt to correlate the IgE, IL-4, and IFN-γ total level of serum in the asthmatics children with disease severity. Fifty one children with acute asthma exacerbation were compare with thirty one no asthmatics normal children matched for age and sex. The level of IgE, IL-4, and IFN-γ were measured using Elisa. Asthma severity was assessed by a symptom score and spirometri. The level of IgE and Il-4 in the serum of the asthma were elevated as compared to the non asthmatic normal controls, whereas the IFN-γ was decreased. There was a significant correlation between elevated levels of IgE and IL-4 with the acute asthma exacerbation severity, whereas the decreased level of IFN-γ was not. Conclusions. The increased levels of IgE , IL-4 and the ratio of  IL-4/IFN-γ play a crucial role in the acute asthma exacerbation severity.
KEMAMPUAN DARI Lactobacillus plantarum GALUR IS-10506 DAN IS-20506 DALAM MENGHAMBAT AKTIVASI NFkB, MEREGULASI TURUN TNF-RECEPTOR 1 (TNF-R1) DAN OPOPTOSIS PADA BRUSH SEL EPITEL BORDER Rattus novergicus YANG DIINDUKSI LPS Kusuma, Titis Sari; Riawan, Wibi; Gunadi Ranuh, I Gusti Made Reza; S. Surono, Ingrid
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1953.998 KB) | DOI: 10.21776/ub.jkb.2008.024.01.3

Abstract

Probiotic bacteria have a beneficial effect on diarrhea. In this study, we have examined effect of Lactobacillus plantarum Strain IS-10506 (LIS-10506) and IS-20506  (LIS-20506) on inhibition of NFκB activation, downregulation TNF Receptor-1 (TNF-R1) and Apoptosis in Epithelial Brush Border of Rattus novergicus induced with LPS. On the first day, LPS was inducedto Rattus novergicus per oral. At the 3rdday, Lactobacillus plantarum Strain IS-10506 and LIS20506 separately were supplemented for 7 days (the 3rdday up to the 9thday). Rat was sacrificed after anaesthetizing with ether to assess NFκB activation, TNFR1 and apoptosis. Result showed the decreases of activation NFκB in LIS-10506 group as well as in LIS-20506 group, significantly different, in NFκB activation at group with only LPS (average 14.33+4.509) compared to group with LIS-10506 induction (average 2.00+1.732)and LIS-20506 induction (average 1.33+1.528), at p( p<0.000). Downregulation of TNF-R1 was significant  at LPS group compared to LIS-10506 induction as well as LIS-20506 induction. The index of apoptosis showed significant of degradation (p<0.000) after induced by LIS, where LPS group (14.67+2.517), LIS-10506 induction (6.33+2.309) and LIS-20506 induction (6.00+3.000). As a conclusion, supplementation of LIS-10506 and LIS-20506 separately will inhibit theNFκB activation, and although the mechanism was not sure, what by significant degrade the expression TNF-R1( as ekuivalen of activity TNFα), and the mentioned give the implication that happened by the degradation of occurence apoptosis at cell of epitel bush border intestine.
DISTRIBUSI MITOKONDRIA DAN EKSPRESI BAX PADA HUVEC’s YANG MENGALAMI APOPTOSIS AKIBAT INDUKSI OX-LDL Riawan, Wibi; Wibowati, Samodrijanti
Jurnal Kedokteran Brawijaya Vol 22, No 2 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.987 KB) | DOI: 10.21776/ub.jkb.2006.022.02.4

Abstract

ABSTRACT It had been revealed that endothelial dysfunction which is caused by oxydized LDL is related to TNF Receptor Apoptosis Including Ligand (TRAIL) mechanism. However, there is study to see the involvement of mitochondrial mecanism in Ox-LDL endothelial cells dysfunction. This study was done to see the effect of Ox-LDL treatment on apoptosis of HUVEC’s culture  whether  associated  with  the  distribution  of  mitochondria  or  BAX  expression,  the  main  protein  mitochondria apoptosis mechanism. HUVEC’s culture was divided into two groups: control group and treatment group ( 50 mg/ml Ox-LDL for 24 hours). Doublestaining for DNA fragmented (TUNEL) and BAX expression were done respectively, as well as immunochemistry  using  monoclonal  Ab  of  mitochondria.  In  the  control  group  mitochondria  was  distributed  firmly  on cytoplasma of endothelial cells. In contrast, mitochondria were only found near nuclear membrane cells on treatment group  (this  future  usually  found  on  divided  cells) there  was  also  an  increasing  of  BAX  expression  and  a  mount  of apoptotic cells on treatment group compared with control group. It can be concluded that mitochondria is involved in apoptosis of Ox-LDL endothelial cells in order to serve the abundant energy. Keywords: Mitochondria distribution, Bax expresson, Apoptotic, Ox-LDL
PROFIL KADAR SOLUBLE UROKINASE PLASMINOGEN ACTIVATOR RECEPTOR (suPAR) PADA SERUM PENDERITA TUBERKULOSIS PARU (SEBAGAI MONITORING TERAPI) Astuti, Triwahju; MR, Tri Yudani; Riawan, Wibi; Muktiati, Nunuk Sri; Widjajanto, Edy
Jurnal Kedokteran Brawijaya Vol 24, No 1 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.873 KB) | DOI: 10.21776/ub.jkb.2008.024.01.4

Abstract

Dutch study shows that Upar expression content is significantly higher in tubercolusis patient’s psiphsy monosit compared to those in the healthy control group. So far, there is no biologic marker used whichcan accurately observe response improvement in the treatment of lung tubercolusis. The aim of this research is to investigate whether the serum level of soluble utokinase plasminogen activator receptor (suPAR) carries information in monitoring TB treatment for Lung Tuberculosis patients. suPAR was measured by ELISA in 21 individuals at the time of enrolment into observational cross sectional based on active tuberculosis  and 5 individuals as healthy control. There were 3 groups, 1). patients who had not started treatment (n=7),2). patients who had been treated for 2 months (n=7),  3). patients who had been treated for 6 months (n=7). Among groups, there were no difference in mean of body mass index, erythrocyte sedimentation rate and monocyte count. Patients positive for TB on direct  microscopy were 29% ( 6 from 21 patients) , 2 patients each groups. The suPAR levels were elevated in patients with active TB compared to healthy control (P<0,001). suPAR levels were highest in patients positive for TB on direct microscopy ( mean suPAR 4.455 ng/ml).
PENGARUH PEMBERIAN OX-LDL TERHADAP AKTIVASI NF-кB DAN PPARγ γγ γSERTA APOPTOSIS PADA KULTUR HUVEC’s Riawan, Wibi; Wibowati, Samodijanti
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (912.71 KB) | DOI: 10.21776/ub.jkb.2006.022.01.5

Abstract

Oxidized LDL (ox-LDL) causes activation and dysfunction of endothelial cells through NFκB activation after binding process of ox-LDL to LOX-1. One of ox-LDL components had been known as the activator of PPARγ. This study was done to see the effect of ox-LDL on NFκB and PPARγactivation and apoptosis of HUVEC’s culture. HUVEC’s culture was divided into 3 groups : control (without any treatment) and 2 groups which were treated with ox-LDL 25ug/mL and 50ug/mL. Activation of NFκB and PPARγwere determined by immunodoublestaining using mouse monoclonal anti NFκB and mouse monoclonal anti PPARγafter 30 minutes exposure of ox-LDL. The expression of TNFαand apoptotic cell were determined by immunodoublestaining using  TUNEL fragmented DNA labeling and goat polyclonal anti human TNFαafter 24 hours exposure of ox-LDL. HUVECs culture that had been treated with 25  µg/ml and 50µg/ml of ox-LDL, showing activation of NFκB but not PPARγ. This treated endotel showed apoptotic characteristic which had conformed with DNA fragmented. These cells also showed the increase of TNFαexpression on the cytoplasm. Ox-LDL could increase translocation of NFκB gen transcription to nucleus followed by the increase of TNFαthat can cause apoptotic of the cells. Key words: ox - LDL, Endothel, NF-κB, PPARγ, Apoptosis
Pemberian Ekstrak Bayam Merah (Amarantus Tricolor linn) Memperbaiki Ekspresi BDNF dan GLUR1 pada Area Hipokampus Tikus Muda yang Terpapar Anestesi Inhalasi Halothane dan Sevoflurane Argarini, Raden; Wigati, Kristanti Wanito; Herawati, Lilik; Riawan, Wibi; Rehatta, Nancy M.
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1578.665 KB) | DOI: 10.15395/mkb.v48n3.844

Abstract

Otak dalam tahap perkembangan memiliki tingkat kerentanan lebih tinggi terhadap zat anestesi dibanding dengan otak orang dewasa. Penelitian ini bertujuan mengamati pengaruh pemberian ekstrak bayam merah terhadap penanda biologis fungsi kognitif dan neuroplastisitas, yaitu Brain Derived Neurotrophic Factor (BDNF) dan AMPA Glutamate Receptor subunit 1 (Glur1) pada area hipokampus tikus yang terpapar anestesi inhalasi halothane dan sevoflurane. Rancangan penelitian adalah randomized posttest only control group. Penelitian ini dilakukan di Departemen Biokimia Fakultas Kedokteran Universitas Airlangga dan prosedur anestesi dilakukan di Rumah Sakit Hewan Fakultas Kedokteran Hewan Universitas Airlangga pada bulan Mei 2014–Oktober 2014. Subjek penelitian adalah 23 ekor tikus putih jantan (Rattus norvegicus) galur Wistar, umur 1–1,5 bulan, bobot kurang lebih 60–100 gram dengan kondisi sehat fisik. Subjek dibagi menjadi 5 kelompok, yaitu K (kontrol); K1 (halothane 1 MAC); K2 (bayam merah 800 mg/kgBB+Halothane 1 MAC); K3 (sevoflurane 1MAC) dan K4 (bayam merah 800 mg/kgBB+ Sevoflurane 1 MAC). Hasil penelitian menunjukkan adanya penurunan ekspresi BDNF pada pemberian halothane dan sevoflurane serta peningkatan kembali ke normal pada kelompok yang sebelumnya telah diberikan bayam merah sebelum terpapar gas anastesi (p=0,000). Ekspresi GLUR1 meningkat pada pemberian halothane dan sevoflurane dan penurunan kembali ke normal pada kelompok yang sebelumnya telah diberikan bayam merah sebelum terpapar gas anestesi (p=0,000). Simpulan penelitian ini adalah pemberian ekstrak bayam merah memiliki potensi untuk mencegah neurotoksisitas pada fungsi kognitif tikus yang terpapar gas anastesi. [MKB. 2016;48(3):148–54]Kata kunci: AMPA Glutamate Receptor subunit 1 (GLUR1), Brain Derived Neurotrophic Factor (BDNF), ekstrak bayam merah, halothane, sevofluraneRed Spinach Extract Administration (Amarantus Tricolor linn) in Young Rats Exposed to Inhalation Anesthetic Halothane and Sevoflurane Improves BDNF and GLUR1 Expressions in Hippocampus AreaIn the development stage, brain has a higher level of vulnerability than the adult brain to anesthetic agents. The aim of this study was to investigate the effects of red amaranth extract to brain derived neurotrophic factor (BDNF) and AMPA glutamate receptor subunit 1 (Glur1) expressions as biomarkers of cognitive and neuroplasticity in rat hippocampus areas exposed to inhaled anesthetics halothane and sevoflurane. The design of this study was a randomized posttest only control group. This study was conducted at the Department of Biochemistry Faculty of Medicine, Universitas Airlangga, and the anesthetic procedures were performed at the Veterinary Hospital, Faculty of Veterinary Medicine Universitas Airlangga during the period of May–October 2014. The subjects of this study were 23 white male rats (Rattus norvegicus) wistar strain, aged 1–1.5 months, weighted approximately 60–100 grams in a healthy physical condition. The subjects were divided into 5 groups, K (control); K1 (1 MAC halothane); K2 (red amaranth 800 mg/kgBW + 1 MAC halothane); K3 (1 MAC sevoflurane); and K4 (red amaranth 800mg/kgBW+1 MAC sevoflurane). The results of this study showed a decrease in the expression of BDNF when halothane and sevoflurane were administered. Red amaranth treatment prior to anesthetic gases exposure preserved and increased BDNF expression (p=0.000) while GLUR1 expression increased in the group that received halothane and sevoflurane administration and decreased back to normal in the group that received red amaranth treatment prior to exposure to anesthetic gases (p=0.000). In conclusion, red amaranth extract has the potential effect to prevent cognitive neurotoxicity on the cognitive function in rats exposed to anesthetic gases. [MKB. 2016;48(3):148–54]Key words: AMPA Glutamate Receptor subunit 1 (GLUR1), Brain Derived Neurotrophic Factor(BDNF), halothane, sevoflurane, red amaranth extract 
Co-Authors Agung Pramana Warih Marhendra, Agung Pramana Warih Alif Fariz Jazmi Alif Fariz Jazmi Almas Dwi Khairana Alpha Fardah Athiyyah Andy Darma Aris Widayati Asnah Hidayat Aulanni'am, Aulanni'am Desie Suci Permata Sari, Desie Suci Edy Widjajanto Erita Rahmani, Erita Erlin Aditia Purmitasari Fedik Abdul Rantam Ferry Sandra Fitri Armania Hendra Susanto HERAWATI, LILIK HMS. Chandra Kusuma I Gusti Made Reza Gunadi Ranuh I Ketut Gede Muliartha Ingrid S. Surono Ingrid Suryanti Surono Kusworini Handono Kalim Laksmi Wulandari Lilin Andreas Vitania Liziyyannida Liziyyannida Lizziyyannida Lizziyyannida, Lizziyyannida Lovita, Agnestia Naning Dian M. R. Indra Machin, Abdulloh Maharani, Afniari Masdar Muid Muslim Akmal Nancy M. Rehatta, Nancy M. Nia Kurniawan Nunuk Sri Muktiati Oktavia Rahayu Adianingsih Putri Fitri Alfiantya Putri Fitri Alfiantya Raden Argarini Ricadonna Raissa Rikardo, Novy Kurnia Samodijanti Wibowati Samodrijanti Wibowati Satya Hanggara Putra Pratama, Satya Hanggara Silvani, Yulia Sitti Ayu Hemas N. Sitti Ayu Hemas Nurarifah SRI RAHAYU Sudarmo, Subijanto Marto Suharti Titis Sari Kusuma Tri Yudani Mardining Raras Triwahju Astuti Wigati, Kristanti Wanito Wildan Mochamad Ridho, Wildan Mochamad Zulkarnaen Zulkarnaen Zurniatus Sholihah, Zurniatus