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Diagnostic Accuracy of Recto-Sigmoid Index Evaluation on Barium Enema in Hirchsprung’s Disease Emma Ratna Fury1 , Andy Darma2 , Alpha Fardah Athiyyah3
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13553

Abstract

Background: Hirschsprung’s disease is a congenital disease caused by the absence of ganglion cellsin the bowel wall, which results in functional obstruction of the aganglionic segment due to failure ofrelaxation during peristalsis. The recto-sigmoid index on barium enema may constitute in the diagnosis ofHirschsprung’s disease.Aim: To evaluate the sensitivity, specificity, positive predictive value, and negative predictive value ofrecto-sigmoid index for the diagnosis of Hirschsprung’s disease in patients who underwent barium enemaexamination.Methods: This retrospective study was conducted in Dr Soetomo Hospital. Medical records of patientssuspected of Hirscsprung’s disease who underwent both barium enema examination and diagnostic fullthickness biopsy were evaluated. Moreover, the sensitivity, specificity, positive predictive value (PPV), andnegative predictive value (NPV) of recto-sigmoid index was calculated.Results: A total of 37 patients were examined; 21 of them were male. The average age was 24.5 (SD 17.34,range 1 month-72 month). Biopsy revealed the absence of ganglion cells in 32 (91,8%) patients. The rectosigmoid index agreed with the histopathologic diagnosis in 30 (81%.) patients. The sensitivity, specificity,PPV, and NPV were 87.5%, 40%, 90.5% and 33% respectively.Conclusion: Recto-sigmoid index evaluation in barium enema for the diagnosis of Hirscsprung’s diseasehas poor diagnostic accuracy.
Proteksi Probiotik pada Mukosa Ileum Mencit yang Terpajan Lipopolisakarida Escherichia Coli Alpha Fardah Athiyyah; Ariani Setiawati; Andy Darma; Anang Endaryanto; I Ketut Sudiana; Reza Ranuh; Subijanto MS
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 2 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.842 KB)

Abstract

Probiotic’s protection in ileal mucosa of mice after lipopolysaccaride Escherichia coliBackground: Gastrointestinal infection is one of the major causes of morbidity in children. Diarrhea is the common manifestation. Diarrheal prevention needs a balance mucosal immune system. Probiotic used in prevention of gastrointestinal infection needs to be considered. The aim of the study is to prove probiotic protection in ileal mucosa after lipopolysaccaride (LPS) Escherichia coli O55:B5 inoculation anatomically and immunologically.Method: Experimental study with male Balb/c, age 10-12 weeks, body weight 30-40g and randomized into treatment group and placebo group. Treatment group received mix probiotic for 14 days and on day 15 were inoculated by LPS Escherichia coli O55:B5, and day 16-21 were given mix probiotic again. Placebo group received LPS Escherichia coli O55:B5 on day 15. Mice necropsy were conducted at day 22. Immunohistochemistry examination used to look for amount expression cell for IL-2, IFN-γ, TNF-α, IL-4, IL-5,IL-6, IL-10 dan TGF-β in ileal mucosa. Scanning electron microscope was used to see ileal mucosal structure. Statistic analysis used in this study was multivariate analysis.Results: Sixteen Balb/c mices were randomized into 2 groups, each group consist 8 mices. There was significant difference on amount of cells expression IL-5 (p=0.022), IL-6 (p=0.05), and also on amount of cells expression TGF-β (p=0.036). On probiotic- LPS group, there was no Th1 domination but on the other hand, Treg became dominant. Th1 and Th2 response were still balance. Structural damages occurred in LPS group and did not occur in probiotic-LPS group.Conclusion: Probiotic protection in ileal Balb/c mice mucosa after LPS Escherichia coli O55:B5 inoculation is through anatomy and immunology changes.Keywords: Probiotic, LPS, Escherichia coli, protection, immunologyABSTRAKLatar belakang: Infeksi gastrointestinal dengan manifestasi tersering diare merupakan salah satu infeksi yang sering terjadi pada anak. Pencegahan diare memerlukan keseimbangan sistem imun mukosa yang baik. Penggunaan probiotik untuk pencegahan terhadap infeksi gastrointestinal perlu dipikirkan. Penelitian ini bertujuan untuk membuktikan proteksi probiotik pada ileum mencit yang terpajan lipopolisakarida (LPS) Escherichia coli O55:B5 secara anatomis dan imunologis.Metode: Uji eksperimental dengan menggunakan mencit Balb/c jantan berusia 10-12 minggu, berat badan 30-40g dan dirandomisasi menjadi kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan mendapatkan mix probiotik selama 14 hari, hari ke-15 mendapatkan LPS Escherichia coli O55:B5 dan hari ke-16-21 kembali mendapatkan mix probiotik. Kelompok kontrol mendapatkan LPS Escherichia coli O55:B5 saja pada hari ke-15. Seluruh mencit dinekropsi pada hari ke-22. Pemeriksaan imunohistokimia untuk melihat ekspresi sel penghasil sitokin IL-2, IFN-, TNF-α, IL-4, IL-5, IL-6, IL-10 dan TGF-ß di jaringan ileum. Scanning microscope electron (SEM) untuk melihat struktur mukosa ileum. Analisis statistik yang digunakan adalah analisis multivariat.Hasil: Enam belas ekor mencit Balb/c terbagi menjadi dua kelompok, masing-masing 8 mencit. Pada kelompok probiotik-LPS didapatkan perbedaan bermakna dibandingkan dengan kelompok LPS pada jumlah penghasil sitokin IL-5 (p=0,022), IL-6 (p=0,050) dan jumlah sel penghasil sitokin TGF-β (p=0,036). Pada kelompok probiotik-LPS, dominasi respon Th1 tidak terlihat lagi dan menimbulkan dominasi Treg pada kelompok ini. Posisi Th1 dan Th2 masih dapat dipertahankan secara seimbang. Kerusakan struktur yang terjadi pada kelompok LPS tidak didapatkan pada kelompok Probiotik-LPS.Simpulan: Proteksi probiotik pada mukosa ileum yang terpajan LPS Escherichia coli terjadi secara anatomi dan imunologi.
THE CORRELATION BETWEEN WATER, SANITATION, AND HYGIENE WITH SOIL-TRANSMITTED HELMINTHS INFECTION AMONG ELEMENTARY SCHOOL CHILDREN OF ARU ISLANDS DISTRICT, MALUKU Tiffany Konstantin; Indah Setyawati Tantular; Alpha Fardah Athiyyah; Lynda Rossyanti
The Indonesian Journal of Public Health Vol. 16 No. 2 (2021): THE INDONESIAN JOURNAL OF PUBLIC HEALTH
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.446 KB) | DOI: 10.20473/ijph.v16i2.2021.273-284

Abstract

ABSTRACTSoil-Transmitted Helminths (STH) infection is the most common intestinal parasite infection in Indonesia. STH infection can interfere with child development and cause cognitive impairment in severe cases.  The school-age group is the most susceptible to STH infection because of direct contact with soil in the school yard. STH infection incidence can be avoided by adequate water, healthy latrines, and good hygiene and can be improved by proper interventions. This study aimed to analyze the correlation between Water, Sanitation, and Hygiene (WASH) with STH infection in Aru Islands District, Maluku. This study used a cross-sectional design and was conducted in Elementary Schools in Karangguli and Wokam villages, Aru Islands district, Maluku. One hundred and six stool samples were collected from both villages. The sociodemographic and WASH data were collected using the interview method. Stool samples were collected and examined for STH infection using a direct smear technique on the light microscope. Data statistical analysis was performed by the SPSS program using the Chi-square or Fischer’s Exact test. The examination results showed that 73 students (68.9%) were infected with STH. The bivariate analysis showed that water source (p = 0.000002), defecation place (p = 0.002), and washing hands after defecation (p = 0.048) were all significantly correlated with STH infection. This study concluded that WASH variables which were water source, defecation place, and washing hands after defecation had a significant correlation with STH infection. Keywords: Water, Sanitation, and Hygiene (WASH); soil-transmitted helminths infection; elementary school students.
Zinc Administration Affects Bronchial Mucosal NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β of Zinc-deficient Rats Reza Gunadi Ranuh; Andy Darma; Alpha Fardah Athiyyah; Ergia Latifolia; Retno Asih Setyoningrum; Boerhan Hidajat; Siti Nurul Hidayati; Anang Endaryanto; Ferry Sandra; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1041

Abstract

BACKGROUND: Risk of acute respiratory infections in children less than 5 years of age is up to 95%. Zinc deficiency is one of the main risk factors. This study aimed to explore the effect of zinc on the bronchial mucosae inflammatory status expressed by nuclear factor (NF)-κB p105/p50, NF-κB p65, interleukin (IL)-8, and IL-1β.METHODS: Twenty-four Wistar rats were divided into 4 groups: normal zinc diet group without zinc supplementation (Z1), normal zinc diet group with zinc supplementation (Z2), zinc deficient diet group without zinc supplementation (Z3), and zinc deficient diet group with zinc supplementation (Z4). NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β were measured by immunohistochemical staining.RESULTS: The inflammatory status of bronchial mucosae between Z1 and Z2 groups showed no difference (p=0.055). However, the inflammatory status of bronchial mucosae between Z3 and Z4 groups showed significant difference (p<0.01). Multivariate factorial design showed that zinc supplementation was beneficial when given to zinc deficient diet group with regard to decrease p-NF-κB p65, IL-8 and IL-1β levels (p<0.001) and increase dendritic cell (p=0.022).CONCLUSION: Zinc administration under conditions of zinc deficiency affects the inflammatory status, as shown by the decrease of p-NF-κB p65, IL-8 and IL-1β and the increase of NF-κB p105/p50.KEYWORDS: zinc, NF-κB, p105/p50, p65, IL-8, IL-1β, rat
Lactobacillus plantarum IS-20506 Probiotic Restores Galectin-4 and Myosin-1a Expressions in Duodenum, Jejunum and Ileum of Lipopolysaccharide-induced Rats Reza Gunadi Ranuh; Alpha Fardah Athiyyah; Andy Darma; Wibi Riawan; Ingrid Suryanti Surono; Ferry Sandra; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1098

Abstract

BACKGROUND: Galectin-4 and Myosin-1a are important proteins for normal intestinal brush border structure and composition. Damage of these proteins by inflammation may alter digestion, absorption and barrier function. Probiotic has been widely known in maintaining gut health. However, the molecular mechanism of Lactobacillus plantarum IS-2056 probiotic in repairing intestinal brush border is not well defined. Therefore, current study was conducted by investigating the Galectin-4 and Myosin-1a expressions in a rodent model.METHODS: Male Wistar rats were induced with/without lipopolysaccharide (LPS) and treated with/without L. plantarum IS-2056 probiotic. On the seventh day, duodenum, jejunum, and ileum were collected and analyzed with western blot and immunohistochemistry for Galectin-4 and Myosin-1a expressions.RESULTS: Rats administrated with L. plantarum IS-2056 probiotic showed significant increase of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum compared to the control group (p<0.05). While in control group, Galectin-4 level tended to increase in more distal of intestinal segment and Myosin-1a level tended to decrease in more distal intestinal segment.CONCLUSION: L. plantarum IS-20506 probiotic may facilitate the repairment of damaged intestinal brush border as demonstrated by significant restoration of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum of LPS-induced rats.KEYWORDS: Lactobacillus plantarum, IS-20506, probiotic, galectin-4, myosin-1a, duodenum, jejunum, ileum
Zinc Supplementation Effect on the Bronchial Cilia Length, the Number of Cilia, and the Number of Intact Bronchial Cell in Zinc Deficiency Rats Andy Darma; Alpha Fardah Athiyyah; Reza Gunadi Ranuh; Wiweka Merbawani; Retno Asih Setyoningrum; Boerhan Hidajat; Siti Nurul Hidayati; Anang Endaryanto; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.998

Abstract

BACKGROUND: Airway epithelium is the first line of defense against a variety of exposures. Inflammatory processes, hyperresponsiveness and zinc deficiency cause epithelial damage. Zinc is involved in apoptosis and microtubule formation. However, its role in the integrity of bronchial mucosa and cilia is unclear.METHODS: To assess the effect of zinc on the integrity of the bronchial epithelium, 24 male Rattus norvegicus strain Wistar rats were randomized into four experimental groups: normal zinc diet group without zinc supplementation, normal zinc diet group with 60 ppm zinc supplementation, zinc deficient diet group without zinc supplementation, and zinc deficient diet group with 120 ppm zinc supplementation. Bronchial mucosal integrity was measured with the number of epithelial cells, and the number and length of cilia.RESULTS: Number of cell in normal zinc diet group was 8.8±1.82, while it was only 8.1±1.08 in zinc deficient diet group (p<0.001). Number of cilia per cell was 4.6±1.08 in normal zinc diet group, compared to 4.0±0.79 in zinc deficient diet group (p<0.001). Ciliary length also differ by 7.68±0.66 μm in normal zinc diet group and only 5.16±0.91 μm in zinc deficient diet group (p<0.001).CONCLUSION: Zinc supplementation of the normal zinc diet group affected the length of bronchial cilia. Zinc supplementation of the zinc deficient diet group affected the integrity of the bronchial epithelium, which was shown by the number and length of cilia, and the number of epithelial cells.KEYWORDS: zinc, bronchial epithelial integrity, cilia length, number of cilia, epithelial cell 
THE CLINICAL EPIDEMIOLOGY OF NOROVIRUS INFECTION IN CHILDREN WITH DIARRHEA AT REGIONAL PUBLIC HOSPITAL DR.SOETOMO Alpha Fardah Athiyyah; Sofia Wardhani; Andy Darma; Reza Gunadi Ranuh; Dadik Raharjo; Toshiro Shirakawa; Subijanto Marto Sudarmo
Jurnal Berkala Epidemiologi Vol. 8 No. 3 (2020): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V8I32020.200-207

Abstract

Background: Norovirus has become a major cause of severe outbreaks of gastroenteritis since the discovery of the rotavirus vaccine, with the main symptom being diarrhea. Until now, research on the epidemiological analysis of norovirus has not been carried out at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya. Purpose: This study aims to provide clinical epidemiology data and an analysis of norovirus infections in children with diarrhea at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya, Indonesia, including the prevalence of norovirus infection in each age group and sex, its clinical appearance, and its seasonal variation. Methods: A cross-sectional study was conducted in children aged 1–60 months hospitalized for diarrhea in RSUD Dr. Soetomo between April 2013 and March 2014. Identification of the virus in the stool was done by norovirus enzyme immunoassay Quick NaviTM Noro2. The proportion, age, sex, clinical symptoms, and patterns of the norovirus seasonal data were calculated. Results: Norovirus was detected in 64 samples (19%) of the 340 stool samples, with a mean patient age of 11.75 months; it was mostly found in patients less than 24 months of age (95%), and 64% were male. The monthly pattern of norovirus infection was mostly found in November, followed by May and April. The clinical symptoms were fever (72%), vomiting (66%), bloating (59%), abdominal cramps (34%), perianal inflammation (27%), abdominal distension (16%), and seizures (8%). Conclusion: The prevalence of norovirus was found to be high in patients with diarrhea aged 1–60 months hospitalized in RSUD Dr. Soetomo, with a proportion of 19%. Further research is needed to determine the severity of norovirus infection.
RISK FACTORS INFLUENCING ENTEROCOLITIS DEVELOPMENT IN PEDIATRIC PATIENTS WITH HIRSCHSPRUNG’S DISEASE Andro Pramana Witarto; Alpha Fardah Athiyyah; I.G.B. Adria Hariastawa; I Gusti Made Reza Gunadi Ranuh
Jurnal Berkala Epidemiologi Vol. 8 No. 3 (2020): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V8I32020.218-227

Abstract

Background: Hirschsprung-associated enterocolitis (HAEC) is one of the worst and most common complications of Hirschsprung’s disease (HD). The mortality rate of HD patients with enterocolitis is still considered to be higher compared to that of those without enterocolitis. Purpose: This study aimed to identify and evaluate potential risk factors for HAEC development. Methods: A cross-sectional study was conducted using secondary data from the medical records of HD patients treated from January 2015 to September 2018 at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya. The inclusion criteria were HD patients who had or had not experienced enterocolitis. The analysis was done by comparing the presence of risk factors between groups of HD patients with and without preoperative and/or postoperative HAEC. The results were presented as the median value and frequency. To evaluate further, a prevalence ratio (PR) with a 95% confidence interval was performed. The Mann–Whitney U test was also performed with a significance level of p < 0.05 for one factor: length of aganglionic intestinal segments. Results: This study showed that 12 of the 40 HD patients studied (30%) had experienced enterocolitis. The risk of developing HAEC was associated with patients who had a history of previous enterocolitis (PR 6.60 [2.94 < PR < 14.80]). Regarding surgical details, patients who had had surgery only once (31.30% compared to 14.30%), surgery with one surgical method (29.40% compared to 20.00%), and a primary procedure had a higher incidence of HAEC (29.40% compared to 27.30%). Conclusion: HD patients with a history of previous enterocolitis were found to have a higher risk of developing HAEC.
A Retrospective Study: Risk Factor Analysis of Secondary Bacterial Infection in Pediatric Atopic Dermatitis Patients Desiana Widityaning Sari; Sawitri Sawitri; Muhammad Yulianto Listiawan; Dwi Murtiastutik; Linda Astari; Alpha Fardah Athiyyah; Afif Nurul Hidayati
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 33 No. 2 (2021): AUGUST
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V33.2.2021.83-87

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Background: Atopic Dermatitis (AD) is a complex multifactorial disease that includes defects in skin architecture, immune dysregulation, and changes of skin flora, and it predominantly occurs in infancy and childhood. The defects in skin barrier structures are mentioned as one of the factors that facilitates bacterial colonization. Bacterial infection in AD can worsen the inflammation. It requires treatment with antibiotics, which takes longer therapy time, higher costs, and ultimately affects the patient’s quality of life and his/her family members. Purpose: To find out the epidemiology, diagnosis, management of AD, and analyze the risk factors of secondary bacterial infection in new AD patients at the Pediatric Dermatology Division, Dermatovenereology Outpatient Clinic, Dr. Soetomo General Academic Hospital. Methods: This was a retrospective study with a total sampling method. The research data were medical records of new AD patients at the Pediatric Dermatology Division, Dermatovenereology Outpatient Clinic, Dr. Soetomo General Academic Hospital Surabaya from January 2012 to December 2018. Result: There were 404 new patients with AD at the Pediatric Dermatology Division from January 2012 to December 2018, and 210 of them were accompanied by secondary bacterial infection. There was a correlation between a history of dry skin (p=0.000) with the incidence of secondary bacterial infection in AD patients. Conclusion: In this study, AD patients with a history of dry skin had a risk of complication such as bacterial secondary infection.
Acute Diarrhea Patients among Children Under Five Hospitalized in a Tertiary Hospital in East Java, Indonesia Asmahan Farah Adiba; Subijanto Marto Soedarmo; Sulistiawati Sulistiawati; Alpha Fardah Athiyyah
Folia Medica Indonesiana Vol. 58 No. 1 (2022): March
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.489 KB) | DOI: 10.20473/fmi.v58i1.31423

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Highlight:The characteristics of acute diarrhea patients among chidren in Tertiary Hospital In East Java was evaluated.The highest incidence of diarrhea was found in the 0-24 months age group, good nutritional status, male sex, and hospitalized in less than 5 days.Mild-moderate dehydration, intravenous rehydration treated, anemia, hyponatremia, and metabolic acidosis were mostly founded. Abstract:Diarrhea is the second leading cause of morbidity and mortality in children under five years of age in Indonesia after pneumonia. In 2017, diarrhea became an outbreak in 12 provinces. Diarrhea in under-fives can cause several complications and can cause death if it is not treated properly. This study aimed to evaluate the characteristics of acute diarrhea patients in under-fives hospitalized in Dr. Soetomo General Academic Hospital, Surabaya, Indonesia for six months. This study was a descriptive study with retrospective approach which evaluated all acute diarrhea patients hospitalized from July to December 2019. Patients’ data were taken from medical records and presented descriptively. Of the total 125 patients, most acute diarrhea patients were male (60%) aged 0 to 24 months (83.2%), had good nutritional status (58.4%), and hospitalized for less than 5 days (60.8%). The most degree of dehydration was mild-moderate dehydration (83.2%), mostly treated with intravenous rehydration (96.8%), the most common comorbidity was anemia (13.4%), and the most electrolyte disorder was hyponatremia (41%), while acid-base disorder was mostly metabolic acidosis (75%).
Co-Authors Adhiningsih, Yunita Ratri Adinda Juwita Syakila Elizafanti Afif Nurul Hidayati Ana Bilah, Aisah Nur Anak Agung Putri Nadia Paramitha Anang Endaryanto Andro Pramana Witarto Andy Darma Antonio Ayrton Widiastara Ariani Setiawati Asmahan Farah Adiba Budi Utomo Budiono Budiono Budiono Budiono BUDIYANTO Cahyanti, Ika Yuniar Christian Susianto, Steven Cita Rosita Sigit Prakoeswa Dadik Rahardjo, Dadik Dadik Raharjo Deasy Fetarayani Desiana Widityaning Sari Dewi Retno Suminar Dwi Murtiastutik Dwiyanti Puspitasari, Dwiyanti DYAH FAUZIAH, DYAH Eko Budi Koendhori, Eko Budi Ergia Latifolia Faradiba, Ailsa Reina Farihazqa Hafez Mikala Fatimah, Nurmawati Ferry Sandra Fitria, Anisa Lailatul Fitriah Gabriel Pedro Mudjianto Gatot Soegiarto Grahana Ade Candra Wolayan Hariastawa, I Gusti Bagus Adria Hengki Anggara Putra Hidajat, Boerhan I Gusti Made Reza Gunadi Ranuh I.G.B. Adria Hariastawa Indah Setyawati Tantular, Indah Setyawati Ingrid Suryanti Surono INGRID SURYANTI SURONO Insyira Rayhana Irsan Juniastuti Juniastuti Kasiyama Desi Indriyani Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Konstantin, Tiffany Laura Navika Yamani Lilik Djuari Linda Astari, Linda Linda Dewanti M. Yulianto Listiawan Maharani, Aussie Tahta Maria Inge Lusida Martha Indah Widia Ningtyas Mira Irmawati Muhammad Faizi Muhammad Faizi, Muhammad Muhammad Irawan Muhammad Miftahussurur Nesa, Ni Nyoman Metriani Ni Nyoman Metriani Nesa Ni Nyoman Metriani Nesa Novi Rahayu Arianti Nur Aisiyah Widjaja, Nur Aisiyah PA, Deanty Ayu PA, Deanty Ayu Raharjo, Dadik Retno Asih Setyoningrum Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Ranuh Reza Ranuh Rohmah, Etik Ainun Ronald Pratama Adiwinoto Rossyanti, Lynda S.Pd. M Kes I Ketut Sudiana . Sacharissa Zerlina Tsarwah Thirafi Sawitri Sawitri Shirakawa, Toshiro Shirakawa, Toshiro SITI NURUL HIDAYATI Sitti Khofifah Yuliana Sofia Wardhani Sriwijayanti Sulistyawati Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Subijanto Marto Soedarmo Subijanto MS Subijanto Subijanto Sudarmo, Subijanto Marto Suharno, Novianto Edi Suhintam Pusarawati Sukmawati Basuki Sulistiawati Sulistiawati Sulistiawati Sumitro, Khadijah Rizky Susianto, Steven Christian Tiffany Konstantin Tiffany Konstantin Toshiro Shirakawa Vina Lidya Setjaputra Wibi Riawan Widati Fatmaningrum Wiweka Merbawani Wolayan, Grahana Ade Candra Yetti Hernaningsih Yunita Ratri Adhiningsih Zayyin Dinana Zunnahri, Salsabila Qurrotul Aini