Indonesian Journal of Biology Education
Vol 3, No 2 (2020): INDONESIAN JOURNAL OF BIOLOGY EDUCATION

Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria

Sukmawati Sukmawati (Faculty of Fisheries, Universitas Muhammadiyah Sorong)
Melda Yunita (Faculty of Medicine, Universitas Pattimura)
Febrianti Rosalina (Faculty of Agriculture, Universitas Muhammadiyah Sorong)



Article Info

Publish Date
01 Feb 2021

Abstract

The bacterium Bacillus subtilis is able to produce amylase enzymes which are able to hydrolyze various types of starch sources into simple compounds such as maltose, glucose. Amylase converts carbohydrates which are polysaccharides into maltose. Amylase enzyme is a group of enzymes that are needed in the industrial field. The purpose of this study was to determine the level of the amylase enzyme produced by the bacterium Bacillus subtilis. The method used in this research is a quantitative method using an amylase enzyme measurement experiment. The results of this study are from two types of enzyme sources, namely Bacillus subtilis + Starch + NB and Bacillus subtilis + NB. Both samples showed enzyme activity. The conclusion of this study is that Bacillus subtilis + Starch + NB produces 63.186 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute. Whereas the Bacillus subtilis + NB sample was 19.474 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute.

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Journal Info

Abbrev

ijobe

Publisher

Subject

Education Social Sciences

Description

Indonesian Journal of Biology Education is a journal managed by Biology Education Study Program, Faculty of Education and Teacher Training, Universitas Tidar. Published articles have been reviewed through a double-blinded review process. This journal is published twice a year, in April and October. ...