Determination of The Active Asiaticoside Content in Centella asiatica as Anti-Cellulite Agent

Sondari, Dewi ( Polymer Chemistry Group, Research Center for Chemistry Indonesian Institute of Sciences (LIPI) Kawasan Puspiptek Serpong, Tangerang 15314 – Indonesia)
Harmami, Sri Budi ( Polymer Chemistry Group, Research Center for Chemistry Indonesian Institute of Sciences (LIPI) Kawasan Puspiptek Serpong, Tangerang 15314 – Indonesia)
Ghozali, M ( Polymer Chemistry Group, Research Center for Chemistry Indonesian Institute of Sciences (LIPI) Kawasan Puspiptek Serpong, Tangerang 15314 – Indonesia)
Randy, Ahmad ( Polymer Chemistry Group, Research Center for Chemistry Indonesian Institute of Sciences (LIPI) Kawasan Puspiptek Serpong, Tangerang 15314 – Indonesia)
S, Athanasia Amanda ( Polymer Chemistry Group, Research Center for Chemistry Indonesian Institute of Sciences (LIPI) Kawasan Puspiptek Serpong, Tangerang 15314 – Indonesia)
Irawan, Yan ( Polymer Chemistry Group, Research Center for Chemistry Indonesian Institute of Sciences (LIPI) Kawasan Puspiptek Serpong, Tangerang 15314 – Indonesia)

Indonesian Journal of Cancer Chemoprevention
Vol 2, No 2 (2011)

Centella  asiatica  accumulates  large  quantities  of  pentacyclic  triterpenoid saponins,  collectively  known  as  centelloids.  These  terpenoids  include  asiaticoside, centelloside,  madecassoside,  brahmoside,  brahminoside,  thankuniside,  sceffoleoside, centellose, asiatic-, brahmic-, centellic- and madecassic acids. Preparations of C. asiatica are  used  in  traditional  and  alternative  medicine  due  to  the  wide  spectrum  of pharmacological  activities  associated  with  these  secondary  metabolites,  such  as anticellulite agent. Asiaticoside was found in Centella asiatica. In this present study, the asiaticoside was extracted using methanolic and ethanolic solvent. Determination of the asiaticoside  content  in  the  extract  was  conducted  with  High Performance  Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), and Fourier Transform Infra Red (FTIR). Samples of C.  asiatica used in this study came from three different plantation  areas,  Bogor,  Lembang  and  Solo.  Asiaticoside  content  in  the  methanolic extract  from  Bogor,  Lembang,  and  Solo  samples  were  2.82%;  2.68%;  and  2.8% respectively. Asiaticoside in ethanolic extract from Bogor, Lembang, and Solo samples were 2.79%; 2.75%; and 2.91% respectively. Two way ANOVA study showed that there was  significant  difference  between  types  of  solvent  used  in  extraction  and  the asiaticoside  content  in  the  obtained  extract,  significant  difference  between  varied plantation area and obtained asiaticoside content, and significant difference between interactions of different solvent with different plantation area.     Key words: Centella asiatica, asiaticoside, anticellulite, medicine, metabolites