Kadar Fenolik Dan Flavonoid Total Dalam Ekstrak Metanol Daun Cemba (Acacia rugata (Lam.) Fawc. Rendle)

Dahlia, Andi Amaliah
Amima, Nur Qadri
S, Aulya Rachma Arum
Syarif, Rezki Amriati
Ahmad, Aktsar Roskiana

Jurnal Fitofarmaka Indonesia
Vol 9, No 1 (2022): JURNAL FITOFARMAKA INDONESIA

This study aims to determine and compare the phenolic and flavonoid content of methanol extract in Acacia rugata (Lam.) Fawc. Rendle leaves using UV-Vis spechtrophotometry and TLC-densitometry. The Acacia rugata (Lam.) Fawc. Rendle extracted by using maceration applied sonicator. The quantitative assay measured by spectrophotometer UV-Vis at wavelength 430 nm. The TLC–densitometry performed at wavelength of 254 nm and quercetin was as a standard. The results showed that the phenolic total showed 0.506 gGAE/g extract for spectrophotometer Uv-Vis and 0.879 gGAE/g extract for TLC-Densitometric. The flavonoid content reported 0.44 gQE/g extract and 0.73 gQE/mg extract for spectrophotometer UV-Vis and TLC Densitometric methods, respectively.