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Kadar Fenolik Dan Flavonoid Total Dalam Ekstrak Metanol Daun Cemba (Acacia rugata (Lam.) Fawc. Rendle) Dahlia, Andi Amaliah; Amima, Nur Qadri; S, Aulya Rachma Arum; Syarif, Rezki Amriati; Ahmad, Aktsar Roskiana
Jurnal Fitofarmaka Indonesia Vol 9, No 1 (2022): JURNAL FITOFARMAKA INDONESIA
Publisher : Jurnal Fitofarmaka Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v9i1.786

Abstract

This study aims to determine and compare the phenolic and flavonoid content of methanol extract in Acacia rugata (Lam.) Fawc. Rendle leaves using UV-Vis spechtrophotometry and TLC-densitometry. The Acacia rugata (Lam.) Fawc. Rendle extracted by using maceration applied sonicator. The quantitative assay measured by spectrophotometer UV-Vis at wavelength 430 nm. The TLC–densitometry performed at wavelength of 254 nm and quercetin was as a standard. The results showed that the phenolic total showed 0.506 gGAE/g extract for spectrophotometer Uv-Vis and 0.879 gGAE/g extract for TLC-Densitometric. The flavonoid content reported 0.44 gQE/g extract and 0.73 gQE/mg extract for spectrophotometer UV-Vis and TLC Densitometric methods, respectively.Â