First report of Banana bunchy top virus on heliconia (Heliconia spp.) in Bali, Indonesia

I Made Arimbawa (Departement of Agricultural Biotechnology, Faculty of Agriculture, Universitas Udayana)
Gusti Ngurah Alit Susanta Wirya (Department of Agroecotechnology, Faculty of Agriculture, Universitas Udayana)
I Putu Sudiarta (Department of Agroecotechnology, Faculty of Agriculture, Universitas Udayana)

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE

Banana bunchy top virus (BBTV) mostly infects Musa spp. which causes banana bunchy top disease (BBTD), the most devastating viral disease in banana cultivation all over the world. During field survey in Sekar Bumi Tropical Farm located in Kerta Village, 15 heliconia plant samples showed green streak on lamina leaves that similar to common BBTD symptoms were collected. Further research was then conducted to identify molecular characteristic of BBTD in heliconia showing green streak. Molecular identification was conducted using polymerase chain reaction (PCR). Specific primers to amplified DNA target were used in this study: mRep/F (5’-GCGTGAAACGCACAAAAGGCC-3’) and mRep/R (5’-GCATACGTTGTCAAACCTTCTCCTC-3’). The expected 240 bp fragment target of partial master replication (mRep) gene was successfully amplified from ten out of 15 samples. Sequence analysis confirmed that the symptomatic heliconia samples were infected with BBTV and falls into the same clade with BBTV from the Asian Group.