JURNAL BUDIDAYA PERTANIAN
Vol 18 No 1 (2022): Jurnal Budidaya Pertanian

Morphological Identification of Five Endophytic Fungus Isolates from Shallots and Their Ability to Inhibit Alternaria porri Ellis Cif

Yetti Elfina (Jurusan Agroteknologi, Fakultas Pertanian, Universitas Riau, Jl. H.R. Soebrantas, Kampus Binawidya, Pekanbaru 28293, Indonesia)
Muhammad Ali (Jurusan Agroteknologi, Fakultas Pertanian, Universitas Riau, Jl. H.R. Soebrantas, Kampus Binawidya, Pekanbaru 28293, Indonesia)
Siti Fatimah Wulandari (Jurusan Agroteknologi, Fakultas Pertanian, Universitas Riau, Jl. H.R. Soebrantas, Kampus Binawidya, Pekanbaru 28293, Indonesia)
Roy Ibrahim (Jurusan Agroteknologi, Fakultas Pertanian dan Peternakan, Universitas Islam Negeri Sultan Syarif Kasim, Jl. HR. Soebrantas Panam No. km 15, Pekanbaru 28293, Indonesia)



Article Info

Publish Date
30 Jun 2022

Abstract

One of the obstacles in shallot cultivation is the attack of purple spot disease caused by the fungus Alternaria porri. Alternative disease control that can be done and is relatively safe for the environment is biological control using indigenous endophytic fungi isolated from the plant to control the disease. Biological control using indigenous microorganisms will be more successful because of their higher compatibility and adaptability. This study aimed to characterize and determine the ability of 5 isolates of endophytic fungi islated fron shallot to control the pathogenic fungus Alternaria porri in vitro. It was carried out through observation and experiment, consisting of: (1) isolation and characterization of endophytic fungi on shallots and (2) in vitro testing of endophytic fungi against A. porri. The data obtained from observations were analyzed descriptively and the data from the inhibitory test were analyzed by analysis of variance, to test five endophytic isolates obtained from shallot root and leaf tissues. The results of this study indicated that: (1) isolate B belonged to the genus Nigrospora, isolate C to the genus Aspergillus, isolate D to the the genus Epicoccum, whereas isolates A and E were unknown; (2) 5 isolates of this indigenous endophytic fungus were hypovirulent with a disease severity index <2 so that they could be used as disease control agents; (3) 5 isolates of indigenous endophytic fungi of shallots showed inhibition ranging from 47.76%-55.25%, with isolate C genus Aspergillus having the highest inhibition with a percentage of 55.25%.

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Journal Info

Abbrev

bdp

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

JURNAL BUDIDAYA PERTANIAN (Journal of Agriculture) first published in 2003 by the Department of Agriculture, Faculty of Agriculture, Pattimura University. Jurnal Budidaya Pertanian is an official publication of the Agriculture Faculty, Pattimura University, publishes primary research paper, review ...