Jurnal Ternak Tropika
Vol 12, No 1 (2011): Ternak Tropika

SUPLEMENTASI FETAL BOVINE SERUM (FBS) TERHADAP PERTUMBUHAN IN VITRO SEL FOLIKEL KAMBING PE

Rahayu, S.N ( Bagian Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya)
Wahjuningsih, S. ( Bagian Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya)



Article Info

Publish Date
24 May 2012

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaansuplementasi FBS dengan berbagai konsentrasi pada medium kultur Tissue CultureMedium-199 (TCM-199) dengan variasi waktu inkubasi terhadap kompetensipertumbuhan folikel kambing PE. Materi Penelitian adalah folikel kambing yangdiperoleh melalui isolasi ovarium kambing, TCM-199 dan FBS. Analisa datamenggunakan uji t berpasangan dengan menggunakan 3 perlakuan suplementasi FBS(0%, 0,1% dan 1%) dan inkubasi selama 6 hari. Folikel yang digunakan dalam kulturdikelompokkan menjadi 2 ukuran yaitu besar (4mm) dan kecil (2-<4mm). Variabelpengamatan yang digunakan adalah pertumbuhan folikel. Pengamatan dilakukan padahari ke 0 dan hari ke 6. Hasil penelitian menunjukkan bahwa suplementasi FBS padakultur folikel secara IVG memberikan pengaruh nyata (P<0,05) terhadap pertumbuhanfolikel, yaitu pada folikel besar dengan suplementasi FBS 1% dan folikel kecil dengansuplementasi FBS 0,1%.Kata Kunci : Fetal Bovine Serum, Folikel, Pertumbuhan In Vitro, KambingFETAL BOVINE SERUM (FBS) SUPPLEMENTATION WITHINCUBATION TIME VARIATION TO FOLLICLE GROWTH COMPETENCEOF PERANAKAN ETAWAH (PE) GOATABSTRACTThe aims of this research was to know the effect of FBS supplements usagewith any concentration of Tissue Culture Medium-199 (TCM-199) culture mediumwith incubation time variation to follicle growth competence of Peranakan Etawah(PE) goat. The material of this research were goat follicle, TCM-199 and FBS. Themethod of this research use t test which used 3 treatment with incubation 6 days. Thefollicle that used in culture were classified into 2 groups: large (>4mm) and small (2-<4mm). The observation variable were used follicle growth. The result of this researchare showed that FBS supplements in follicle culture according to IVG gave thesignificant different into follicle growth (P<0,05) that is large follicle with 1% FBSsupplements and small follicle with 0,1% FBS supplements. It can be concluded thatFBS supplements and length of culture in IVG follicle had the significant influenceon the growth of follicles.Keywords: Fetal Bovine Serum,Follicle, In Vitro Growth,Goat

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Journal Info

Abbrev

TROPIKA

Publisher

Subject

Description

TERNAK TROPIKA, Journal of Tropical Animal Production (JTAP) provides for rapid publication of full-length papers, short communication and review articles describing of new finding or theory in Animal Production area. TERNAK TROPIKA has 1 volume with 2 issues per year. TERNAK TROPIKA is published ...